摘要
This work presents a promising clinical molecular diagnostics for early stage lung cancer. This novel diagnostic method utilized the loopmediated isothermal amplification （LAMP）, micro fluidic chips and a confocal optical detector with a nonlinear fluorescent filter processor. An isothermal amplification based microfluidic chip for the early diagnostics of lung cancer was developed and a confocal optical detector was improved by a novel real-time fluorescent filter to sensitively monitor the DNA amplification procedure with high signal to noise ratio and fluorescence collecting ability. Experiment showed that a rapid diagnostic of lung cancer by detecting the existence of the CEA mRNA could be performed in only 5 laL of reaction assay in less than 45 min. While the traditional intube RTPCR set consumed more than 25 -μL of the assay and took more than 90 rain.
This work presents a promising clinical molecular diagnostics for early stage lung cancer. This novel diagnostic method utilized the loop-mediated isothermal amplification (LAMP), microfluidic chips and a confocal optical detector with a non-linear fluorescent filter processor. An isothermal amplification based microfluidic chip for the early diagnostics of lung cancer was developed and a confocal optical detector was improved by a novel real-time fluorescent filter to sensitively monitor the DNA amplification procedure with high signal to noise ratio and fluorescence collecting ability. Experiment showed that a rapid diagnostic of lung cancer by detecting the existence of the CEA mRNA could be performed in only 5 μL of reaction assay in less than 45 min. While the traditional in-tube RT-PCR set consumed more than 25 μL of the assay and took more than 90 min.
作者
WANG TongZhou1, ZHANG Ye1, HUANG GuoLiang1,2,3, WANG Can2, XIE Lan1, MA Li2, LI ZhiYong2, LUO XianBo2, TIAN Hao2, LI Qiang1, LI Xin1, LV ZhouYan1 & BAO XiaoFan11Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing 100084, China 2National Engineering Research Center for Beijing Biochip Technology, Beijing 102206, China 3Yangtze Delta Region Institute of Tsinghua University, Hangzhou 330401, China
基金
supported by the National Supporting Plan of China (2011BAZ03288, 2012BAI23B00)
the National Program on Key Basic Research Projects of China (2011CB707701)
the National Natural Science Foundation of China (60978064)
the Science Plan Foundation of Zhejiang (2012C31G3210006)
the TSINGHUA-SIEMENS Collective Foundation (20103000273)
