目的观察甘草黄酮对S180和H22荷瘤小鼠肿瘤细胞DNA及RNA的影响。方法S180和H22荷瘤小鼠随机分为甘草黄酮高、中、低剂量(25、11.25、5.58 m g/kg)组,阳性对照(环磷酰胺25 m g/kg)组和阴性对照(生理盐水)组,各组均sc给药。运用激光扫描...目的观察甘草黄酮对S180和H22荷瘤小鼠肿瘤细胞DNA及RNA的影响。方法S180和H22荷瘤小鼠随机分为甘草黄酮高、中、低剂量(25、11.25、5.58 m g/kg)组,阳性对照(环磷酰胺25 m g/kg)组和阴性对照(生理盐水)组,各组均sc给药。运用激光扫描共聚焦显微镜技术与吖啶橙(AO)荧光探针技术结合检测单一肿瘤细胞DNA和RNA的变化情况。结果不同剂量甘草黄酮组和环磷酰胺组的DNA及RNA荧光强度较生理盐水组有不同程度的减弱;甘草黄酮高、低剂量组RNA/DAN值与生理盐水组比较差异无显著性;甘草黄酮中剂量组、环磷酰胺组RNA/DNA值较生理盐水组有非常显著提高(P<0.01)。结论甘草黄酮与肿瘤细胞DNA和RNA结合后抑制其进一步复制与合成,可能是其抗肿瘤作用的途径之一。展开更多
Objective To estimate the predictive value of double fluorescence [acridine orange （AO）/propidium iodide （PI）] staining results for fertilization rate and clinical outcomes and analyze the correlation between th...Objective To estimate the predictive value of double fluorescence [acridine orange （AO）/propidium iodide （PI）] staining results for fertilization rate and clinical outcomes and analyze the correlation between the results of AO/P1 staining and sperm apoptosis. Methods A prospective study was carried out, 235 infertile couples remedied using traditional in vitro fertilization （IVF） were included. Semen collected from 235 patients were stained by fluorescence dye, AO and PL at the same time the spermatozoa apoptosis rate was calculated by using flow cytometry to detect the rate of Annexin V＋/ PI- . The result of fluorescence was divided into 3 groups as green （G）, yellow （Y） and red （R） according to the color of fluorescence. The correlation between the percent of the 3 colors and clinical outcomes and spermatozoa apoptosis rate was evaluated. Results Significant negative correlation was observed between the percentage of Y and fertilization rate （r= -0.42, P=0.04）, no significant correlation was observed between the percentage of G, R and fertilization rate. The percentage of G, Y, R was not significantly different between pregnant patients and non-pregnant patients, respectiw,ly, while the percentage of Y was significant different between miscarriage patients and liveborn patients （r=0.6L P=0.01） and no significant difference exist in the percentage of G and R between liveborn patients and miscarriage patients. There was a significant positive correlation between the percentage of Y and the rate of Annexin V＋/ PI （r=0.53, P=0.04）, and no significant correlation was shown between the percentage of G, R and the rate of Annexin V＋/ PI-.Conclusion The percentage of yellow group of double fluorescence （AO/PI） staining will affect the fertilization rate and the miscarriage rate, and this group of spermatozoa may be connected with the spermatozoa apoptosis.展开更多
文摘目的观察甘草黄酮对S180和H22荷瘤小鼠肿瘤细胞DNA及RNA的影响。方法S180和H22荷瘤小鼠随机分为甘草黄酮高、中、低剂量(25、11.25、5.58 m g/kg)组,阳性对照(环磷酰胺25 m g/kg)组和阴性对照(生理盐水)组,各组均sc给药。运用激光扫描共聚焦显微镜技术与吖啶橙(AO)荧光探针技术结合检测单一肿瘤细胞DNA和RNA的变化情况。结果不同剂量甘草黄酮组和环磷酰胺组的DNA及RNA荧光强度较生理盐水组有不同程度的减弱;甘草黄酮高、低剂量组RNA/DAN值与生理盐水组比较差异无显著性;甘草黄酮中剂量组、环磷酰胺组RNA/DNA值较生理盐水组有非常显著提高(P<0.01)。结论甘草黄酮与肿瘤细胞DNA和RNA结合后抑制其进一步复制与合成,可能是其抗肿瘤作用的途径之一。
基金supported by a grant from STCSM (Subject No.:074207002)
文摘Objective To estimate the predictive value of double fluorescence [acridine orange （AO）/propidium iodide （PI）] staining results for fertilization rate and clinical outcomes and analyze the correlation between the results of AO/P1 staining and sperm apoptosis. Methods A prospective study was carried out, 235 infertile couples remedied using traditional in vitro fertilization （IVF） were included. Semen collected from 235 patients were stained by fluorescence dye, AO and PL at the same time the spermatozoa apoptosis rate was calculated by using flow cytometry to detect the rate of Annexin V＋/ PI- . The result of fluorescence was divided into 3 groups as green （G）, yellow （Y） and red （R） according to the color of fluorescence. The correlation between the percent of the 3 colors and clinical outcomes and spermatozoa apoptosis rate was evaluated. Results Significant negative correlation was observed between the percentage of Y and fertilization rate （r= -0.42, P=0.04）, no significant correlation was observed between the percentage of G, R and fertilization rate. The percentage of G, Y, R was not significantly different between pregnant patients and non-pregnant patients, respectiw,ly, while the percentage of Y was significant different between miscarriage patients and liveborn patients （r=0.6L P=0.01） and no significant difference exist in the percentage of G and R between liveborn patients and miscarriage patients. There was a significant positive correlation between the percentage of Y and the rate of Annexin V＋/ PI （r=0.53, P=0.04）, and no significant correlation was shown between the percentage of G, R and the rate of Annexin V＋/ PI-.Conclusion The percentage of yellow group of double fluorescence （AO/PI） staining will affect the fertilization rate and the miscarriage rate, and this group of spermatozoa may be connected with the spermatozoa apoptosis.
