摘要
介绍了近几年荧光能量转移体系用于 DNA分析测定方法的进展 .通过实验对吖啶橙( AO)—藏红 T( ST)荧光体系能量转移进行分析 ,说明使用该本系为荧光探针对 DNA定量测定的可行性 .然后通过 Forster能量转移理论 ,计算 DNA加入前后 AO- ST体系临界距离变化 ,从某种程度上说明 DNA对 AO- ST荧光淬灭的影响 .
This paper introduces recent progress in method of applying fluorescence energy transfer system to the analytic test of DNA,the energy transfer of Acridine orange (AO) Safranine (ST) System is analyzed through experiments,proving the possibility of quantatively testing DNA with the fluorescene probe of this system,critical ranges of AO-ST before and after the addition of DNA are calculated respectively, according to Forster theory of energy transfer,thus to some extent illustrating the influence of DNA on AO ST fluoresence quenching
出处
《新疆大学学报（自然科学版）》
CAS
2000年第4期33-39,共7页
Journal of Xinjiang University(Natural Science Edition)
关键词
能量转移
吖啶橙
藏红
DNA
荧光分析
Fluorescene energy transfer
fluorescenes quenching
acridine orange(AO) Safranine T(ST)
critical range
fluoresonce probe
