目的探讨急性脑梗死患者颈动脉粥样硬化斑块的稳定性与血管内皮功能的相关性。方法应用颈动脉彩色多普勒超声技术检查研究对象颈动脉粥样硬化斑块的大小、数目及性质。选取血管性假血友病因子(Von Willebrand factor,vWF)与可溶性血管...目的探讨急性脑梗死患者颈动脉粥样硬化斑块的稳定性与血管内皮功能的相关性。方法应用颈动脉彩色多普勒超声技术检查研究对象颈动脉粥样硬化斑块的大小、数目及性质。选取血管性假血友病因子(Von Willebrand factor,vWF)与可溶性血管内皮细胞蛋白C受体(Soluble endothelial protein C receptor,sEPCR)作为急性脑梗死患者血管内皮功能的评价指标。采用ELISA方法检测其血浆中vWF与sEPCR含量。根据颈动脉彩超的结果,将急性脑梗死患者分为不稳定性斑块脑梗死组、稳定性斑块脑梗死组,并选取健康对照组,组间比较采用独立样本t检验,并行简单相关分析。结果不稳定性斑块脑梗死组血浆中vWF与sEPCR含量显著高于稳定性斑块脑梗死组(P<0.001),急性脑梗死患者颈动脉粥样硬化斑块的稳定性与vWF及sEPCR呈负相关(P<0.001)。结论急性脑梗死患者血管内皮功能损伤越严重,则颈动脉粥样硬化斑块可能越不稳定。展开更多
目的通过比较人内皮细胞特异性分子-1(endothelial cell specific molecule-1,endocan)、血管性血友病因子(von Willebrand factor,vWF)、含Ⅰ型血小板结合蛋白基序的解聚蛋白样金属蛋白酶(A disintegrin-like and metalloprotease with...目的通过比较人内皮细胞特异性分子-1(endothelial cell specific molecule-1,endocan)、血管性血友病因子(von Willebrand factor,vWF)、含Ⅰ型血小板结合蛋白基序的解聚蛋白样金属蛋白酶(A disintegrin-like and metalloprotease with thrombospondin type 1 motif,ADAMTS-13)及vWF/ADAMTS-13的循环水平在急诊抢救室全身炎症反应综合征(SIRS)、脓毒症、脓毒症休克患者及健康对照人群之间的差异,评价以上各个指标对于脓毒症患者危险分层、病情严重程度的评估能力。方法收集从2014年10月到2015年10月北京朝阳医院急诊抢救室符合2001年脓毒症定义会议制定的符合SIRS或脓毒症诊断标准患者301例。根据诊断标准分为SIRS组,脓毒症组,严重脓毒症组和脓毒症休克组。选择健康体检者40例作为对照组。检测入选者endocan、vWF、ADAMTS-13、vWF/ADAMTS-13、PCT水平,并进行急性生理学和慢性健康状况评分(acute physiology and chronic health evaluation,APACHEⅡ)、急诊室脓毒症死亡风险评分(mortality in emergency department sepsis,MEDS)和序贯器官功能衰竭评分(sequential organ failure assessment,SOFA)评分。记录28 d随访时间点患者的全因死亡或存活情况。结果endocan、vWF、vWF/ADAMTS-13水平在SIRS、脓毒症、严重脓毒症及脓毒症休克患者中显著增高,与疾病的严重程度呈正相关;并与MEDS评分、APACHEⅡ评分和SOFA评分之间均具有显著的正相关。ADAMTS-13水平则随着病情的加重而逐渐降低,与疾病的严重程度呈负相关;并与各评分系统之间具有显著的负相关。结论endocan、vWF、ADAMTS-13、vWF/ADAMTS-13在脓毒症的危险分层、病情评估均有一定的临床价值,可以作为新型的脓毒症生物标记物应用于临床。展开更多
ECV304 was reported first in 1990 as a spont aneously-transformed and immortalized cell line derived from a Japanese HUVEC. S ubsequently, many studies validated that the ECV304 is a permanent endothelial cell line. I...ECV304 was reported first in 1990 as a spont aneously-transformed and immortalized cell line derived from a Japanese HUVEC. S ubsequently, many studies validated that the ECV304 is a permanent endothelial cell line. It has been used widely as an endothelial cell model and an useful re search tool in biomedicine and pharmacology. However, several distinct differenc es exist between ECV304 and HUVEC. Some studies even pointed out that ECV304 is not of HUVEC origin. According to the research data including ours, this reporte dly endothelial-derived permanent human cell line ECV304 may be dedifferentiated towards an epithelial phenotype. It is therefore not an appropriate cell line t o study endothelial cell biology. But cultured ECV304 cells can still be used as a model, tool or target in the pathophysiological and pharmacological studies, depending on whether or not their functional expression or markers are suitable for the research work.展开更多
Objective:To explore the effect of ginkgo leaf extract(GLE) on vascular endothelial function(VEF) in patients with early stage diabetic nephropathy(DN).Methods:Sixty-four patients were randomized equally by a randomzi...Objective:To explore the effect of ginkgo leaf extract(GLE) on vascular endothelial function(VEF) in patients with early stage diabetic nephropathy(DN).Methods:Sixty-four patients were randomized equally by a randomzing digital table into two groups,the treated group and the control group.They were all treated for 8 weeks with conventional therapy for diabetes,but GLE tablets were given to the treated group additionally. Changes in VEF were estimated before and after treatment by ultrasonic examination of t...展开更多
文摘目的探讨急性脑梗死患者颈动脉粥样硬化斑块的稳定性与血管内皮功能的相关性。方法应用颈动脉彩色多普勒超声技术检查研究对象颈动脉粥样硬化斑块的大小、数目及性质。选取血管性假血友病因子(Von Willebrand factor,vWF)与可溶性血管内皮细胞蛋白C受体(Soluble endothelial protein C receptor,sEPCR)作为急性脑梗死患者血管内皮功能的评价指标。采用ELISA方法检测其血浆中vWF与sEPCR含量。根据颈动脉彩超的结果,将急性脑梗死患者分为不稳定性斑块脑梗死组、稳定性斑块脑梗死组,并选取健康对照组,组间比较采用独立样本t检验,并行简单相关分析。结果不稳定性斑块脑梗死组血浆中vWF与sEPCR含量显著高于稳定性斑块脑梗死组(P<0.001),急性脑梗死患者颈动脉粥样硬化斑块的稳定性与vWF及sEPCR呈负相关(P<0.001)。结论急性脑梗死患者血管内皮功能损伤越严重,则颈动脉粥样硬化斑块可能越不稳定。
文摘目的通过比较人内皮细胞特异性分子-1(endothelial cell specific molecule-1,endocan)、血管性血友病因子(von Willebrand factor,vWF)、含Ⅰ型血小板结合蛋白基序的解聚蛋白样金属蛋白酶(A disintegrin-like and metalloprotease with thrombospondin type 1 motif,ADAMTS-13)及vWF/ADAMTS-13的循环水平在急诊抢救室全身炎症反应综合征(SIRS)、脓毒症、脓毒症休克患者及健康对照人群之间的差异,评价以上各个指标对于脓毒症患者危险分层、病情严重程度的评估能力。方法收集从2014年10月到2015年10月北京朝阳医院急诊抢救室符合2001年脓毒症定义会议制定的符合SIRS或脓毒症诊断标准患者301例。根据诊断标准分为SIRS组,脓毒症组,严重脓毒症组和脓毒症休克组。选择健康体检者40例作为对照组。检测入选者endocan、vWF、ADAMTS-13、vWF/ADAMTS-13、PCT水平,并进行急性生理学和慢性健康状况评分(acute physiology and chronic health evaluation,APACHEⅡ)、急诊室脓毒症死亡风险评分(mortality in emergency department sepsis,MEDS)和序贯器官功能衰竭评分(sequential organ failure assessment,SOFA)评分。记录28 d随访时间点患者的全因死亡或存活情况。结果endocan、vWF、vWF/ADAMTS-13水平在SIRS、脓毒症、严重脓毒症及脓毒症休克患者中显著增高,与疾病的严重程度呈正相关;并与MEDS评分、APACHEⅡ评分和SOFA评分之间均具有显著的正相关。ADAMTS-13水平则随着病情的加重而逐渐降低,与疾病的严重程度呈负相关;并与各评分系统之间具有显著的负相关。结论endocan、vWF、ADAMTS-13、vWF/ADAMTS-13在脓毒症的危险分层、病情评估均有一定的临床价值,可以作为新型的脓毒症生物标记物应用于临床。
文摘ECV304 was reported first in 1990 as a spont aneously-transformed and immortalized cell line derived from a Japanese HUVEC. S ubsequently, many studies validated that the ECV304 is a permanent endothelial cell line. It has been used widely as an endothelial cell model and an useful re search tool in biomedicine and pharmacology. However, several distinct differenc es exist between ECV304 and HUVEC. Some studies even pointed out that ECV304 is not of HUVEC origin. According to the research data including ours, this reporte dly endothelial-derived permanent human cell line ECV304 may be dedifferentiated towards an epithelial phenotype. It is therefore not an appropriate cell line t o study endothelial cell biology. But cultured ECV304 cells can still be used as a model, tool or target in the pathophysiological and pharmacological studies, depending on whether or not their functional expression or markers are suitable for the research work.
基金Supported by the Scientific Research Grant of Zhejiang Provincial Administration Bureau of Education(No.20051319)
文摘Objective:To explore the effect of ginkgo leaf extract(GLE) on vascular endothelial function(VEF) in patients with early stage diabetic nephropathy(DN).Methods:Sixty-four patients were randomized equally by a randomzing digital table into two groups,the treated group and the control group.They were all treated for 8 weeks with conventional therapy for diabetes,but GLE tablets were given to the treated group additionally. Changes in VEF were estimated before and after treatment by ultrasonic examination of t...
