通过对米曲霉菌株进行紫外线诱变处理,采用快速显色的平板初筛方法,得到三株曲酸产量较高和产酸性能比较稳定的米曲霉突变菌株,并从中筛选出产酸能力最高的米曲霉菌株10V-2。经对该菌株发酵条件进行初步研究,找出了该菌株的最适发酵产...通过对米曲霉菌株进行紫外线诱变处理,采用快速显色的平板初筛方法,得到三株曲酸产量较高和产酸性能比较稳定的米曲霉突变菌株,并从中筛选出产酸能力最高的米曲霉菌株10V-2。经对该菌株发酵条件进行初步研究,找出了该菌株的最适发酵产酸培养条件,使其摇瓶发酵曲酸产量达到30.51 m g/L。展开更多
The basic methods of concentration and purification of liquid radioactive waste on the nuclear power plant are distillation and ionic exchange. During vaporization of oil waste products and the fulfilled washing solut...The basic methods of concentration and purification of liquid radioactive waste on the nuclear power plant are distillation and ionic exchange. During vaporization of oil waste products and the fulfilled washing solutions the part of oil passes into a condensate. Clearing of such condensate on ion-exchanged filters results to oiling of ion-exchanged materials and to decrease number of filter cycles. Due to the often regeneration of ion-exchanged filters additional volumes of waste products as the fulfilled reclaiming solutions, washing and loosening waters are formed. The attention of scientists was involved with methods of clearing of water environments from the organic substances, based on deep oxidizing transformations of hydrocarbons into carbonic gas and water. From processes of oxidation of hydrocarbons up to CO2 and H2O, sold at moderate conditions, our attention has involved photochemical oxidation with the help of UV-radiation.展开更多
AIM: To explore the protective effect of zeaxanthin on human limbal and conjunctival epithelial cells against UVradiation and excessive oxidative stress.METHODS: Human limbal and conjunctival epithelial cells were iso...AIM: To explore the protective effect of zeaxanthin on human limbal and conjunctival epithelial cells against UVradiation and excessive oxidative stress.METHODS: Human limbal and conjunctival epithelial cells were isolated from cadaver and cultured in vitro. They were challenged with UVB radiation and H2 O2 with and without zeaxanthin pretreatment. Cell viability, p38 and c-JUN NH(2)-terminal kinase(JNK) phosphorylation, IL-6, IL-8 and MCP-1 secretion and malondialdehyde(MDA) content were measured.RESULTS: Zeaxanthin had no measurable cytotoxicity on limbal or conjunctival epithelial cells when used at concentrations of 5 μg/mL and below. At 30 mJ/cm2 UVB, the pretreatment of zeaxanthin increased the percentage of live cells from 50% to 69%(P=0.01) and from 66% to 75%(P=0.05) for limbal and conjunctival epithelial cells, respectively. The concentrations of IL-6, IL-8 and MCP-1 in the culture medium reduced to 66%(for IL-6 and MCP-1)and 56%(for IL-8) of the levels without zeaxanthin. This was accompanied by reduced p38 and JNK protein phosphorylation. Pretreatment of zeaxanthin also reduced intracellular MDA content caused by H2 O2 stimulation from 0.86 μmol/L to 0.52 μmol/L(P=0.02) in limbal epithelial cells and from 0.96 μmol/L to 0.56 μmol/L in conjunctival epithelial cells(P=0.03). However, zeaxanthin did nothave significant effect on H2 O2-induced cell death in limbal or conjunctival epithelial cells.CONCLUSION: Zeaxanthin is an effective reagent in reducing the detrimental effect of UV-radiation and oxidative stress on ocular surface epithelial cells.展开更多
Geosynthetics used as geotextiles/geomembranes lose their mechanical properties when exposed to outdoor solar radiation. The UV-component present in the solar radiation is main cause for such degradation of mechanical...Geosynthetics used as geotextiles/geomembranes lose their mechanical properties when exposed to outdoor solar radiation. The UV-component present in the solar radiation is main cause for such degradation of mechanical properties. The life of geosynthetics has to be predicted before the intended purpose by exposing the material under artificial UV-radiation in the laboratory. Simulation of UV-radiation has been set up for accelerated ageing condition of Mumbai climate.展开更多
文摘The basic methods of concentration and purification of liquid radioactive waste on the nuclear power plant are distillation and ionic exchange. During vaporization of oil waste products and the fulfilled washing solutions the part of oil passes into a condensate. Clearing of such condensate on ion-exchanged filters results to oiling of ion-exchanged materials and to decrease number of filter cycles. Due to the often regeneration of ion-exchanged filters additional volumes of waste products as the fulfilled reclaiming solutions, washing and loosening waters are formed. The attention of scientists was involved with methods of clearing of water environments from the organic substances, based on deep oxidizing transformations of hydrocarbons into carbonic gas and water. From processes of oxidation of hydrocarbons up to CO2 and H2O, sold at moderate conditions, our attention has involved photochemical oxidation with the help of UV-radiation.
基金Supported by the National Natural Science Foundation of China(No.81570829)
文摘AIM: To explore the protective effect of zeaxanthin on human limbal and conjunctival epithelial cells against UVradiation and excessive oxidative stress.METHODS: Human limbal and conjunctival epithelial cells were isolated from cadaver and cultured in vitro. They were challenged with UVB radiation and H2 O2 with and without zeaxanthin pretreatment. Cell viability, p38 and c-JUN NH(2)-terminal kinase(JNK) phosphorylation, IL-6, IL-8 and MCP-1 secretion and malondialdehyde(MDA) content were measured.RESULTS: Zeaxanthin had no measurable cytotoxicity on limbal or conjunctival epithelial cells when used at concentrations of 5 μg/mL and below. At 30 mJ/cm2 UVB, the pretreatment of zeaxanthin increased the percentage of live cells from 50% to 69%(P=0.01) and from 66% to 75%(P=0.05) for limbal and conjunctival epithelial cells, respectively. The concentrations of IL-6, IL-8 and MCP-1 in the culture medium reduced to 66%(for IL-6 and MCP-1)and 56%(for IL-8) of the levels without zeaxanthin. This was accompanied by reduced p38 and JNK protein phosphorylation. Pretreatment of zeaxanthin also reduced intracellular MDA content caused by H2 O2 stimulation from 0.86 μmol/L to 0.52 μmol/L(P=0.02) in limbal epithelial cells and from 0.96 μmol/L to 0.56 μmol/L in conjunctival epithelial cells(P=0.03). However, zeaxanthin did nothave significant effect on H2 O2-induced cell death in limbal or conjunctival epithelial cells.CONCLUSION: Zeaxanthin is an effective reagent in reducing the detrimental effect of UV-radiation and oxidative stress on ocular surface epithelial cells.
文摘Geosynthetics used as geotextiles/geomembranes lose their mechanical properties when exposed to outdoor solar radiation. The UV-component present in the solar radiation is main cause for such degradation of mechanical properties. The life of geosynthetics has to be predicted before the intended purpose by exposing the material under artificial UV-radiation in the laboratory. Simulation of UV-radiation has been set up for accelerated ageing condition of Mumbai climate.
