In weak acid medium,a significant enhancement of resonance Rayleigh scattering(RRS) spectrum and new RRS spectra were observed when amikacin reacted with acid bisazo dye trypan blue and new RRS spectra with amaximum p...In weak acid medium,a significant enhancement of resonance Rayleigh scattering(RRS) spectrum and new RRS spectra were observed when amikacin reacted with acid bisazo dye trypan blue and new RRS spectra with amaximum peak at 717nm.The characteristic of RRS spectra,the optimum conditions for the reaction and the influencing factors were studied.The concentration of amikacin in the range of 0~6.24μg/mL is linear to the resonance scattering intensity(IRRS)at 717nm.The detection limit(3σ) of this method for Amikacin Sulphate is 9.2 ng·mL-1.Therefore,a simple,high sensitivity,good selectivity and repeat method for the determination of Amikacin Sulphate injection samples by RRS is developed with satisfactory results.展开更多
Objective:To investigate anticancer activity of different fractions of Tephrosia purpurea[TP] (Sharapunkha,Fabaceae) and Ficus religiosa[FR](Peepal,Moraceae).Methods:The fractions of TP and FR were prepared and te...Objective:To investigate anticancer activity of different fractions of Tephrosia purpurea[TP] (Sharapunkha,Fabaceae) and Ficus religiosa[FR](Peepal,Moraceae).Methods:The fractions of TP and FR were prepared and tested for in vitro anticancer activity using human MCF 7 cell line by trypan blue exclusion method.Results:The result showed that among all these fractions of TPI.TPIII.FRI and FRIII showed better anticancer activity compared to other fractions.The IC<sub>50</sub> value for TPI(152.4μM),TPIII(158.71μM).FRI(160.3μM) and for FRIII(222.7μM) was observed.Conclusions:The present study shows anticancer potential of TP and FR fractions in MCF 7 cell line.展开更多
文摘In weak acid medium,a significant enhancement of resonance Rayleigh scattering(RRS) spectrum and new RRS spectra were observed when amikacin reacted with acid bisazo dye trypan blue and new RRS spectra with amaximum peak at 717nm.The characteristic of RRS spectra,the optimum conditions for the reaction and the influencing factors were studied.The concentration of amikacin in the range of 0~6.24μg/mL is linear to the resonance scattering intensity(IRRS)at 717nm.The detection limit(3σ) of this method for Amikacin Sulphate is 9.2 ng·mL-1.Therefore,a simple,high sensitivity,good selectivity and repeat method for the determination of Amikacin Sulphate injection samples by RRS is developed with satisfactory results.
文摘Objective:To investigate anticancer activity of different fractions of Tephrosia purpurea[TP] (Sharapunkha,Fabaceae) and Ficus religiosa[FR](Peepal,Moraceae).Methods:The fractions of TP and FR were prepared and tested for in vitro anticancer activity using human MCF 7 cell line by trypan blue exclusion method.Results:The result showed that among all these fractions of TPI.TPIII.FRI and FRIII showed better anticancer activity compared to other fractions.The IC<sub>50</sub> value for TPI(152.4μM),TPIII(158.71μM).FRI(160.3μM) and for FRIII(222.7μM) was observed.Conclusions:The present study shows anticancer potential of TP and FR fractions in MCF 7 cell line.