目的:探讨大鼠气管上皮损伤后,气管干细胞增殖分化过程中Shh信号通路的动态变化。方法:应用5-氟尿嘧啶(5-FU)诱发气管上皮损伤,通过RT-PCR检测气管上皮Shh的表达。结果:5-FU作用30 m in后大鼠气管上皮细胞绝大部分脱落,仅残留少量裸核...目的:探讨大鼠气管上皮损伤后,气管干细胞增殖分化过程中Shh信号通路的动态变化。方法:应用5-氟尿嘧啶(5-FU)诱发气管上皮损伤,通过RT-PCR检测气管上皮Shh的表达。结果:5-FU作用30 m in后大鼠气管上皮细胞绝大部分脱落,仅残留少量裸核样G0期细胞位于基底膜上。之后细胞数目逐渐增多,经过扁平、立方形态,向纤毛细胞、黏液细胞、基底细胞等分化,去除5-FU后48 h接近恢复假复层纤毛柱状上皮;正常气管上皮及5-FU作用30 m in气管上皮Shh无表达,去除5-FU恢复3 h后Shh轻度表达,6 h后表达增强,12 h达高峰,之后表达减弱,48 h已极微弱。结论:Shh参与了气管干细胞的增殖分化过程。展开更多
Introduction:Traumatic neck injuries(TNIs)constitute 5%–10%of all trauma cases.These injuries can be caused by either penetrating or blunt trauma.Patients can have a varied presentation like cut injury over the neck,...Introduction:Traumatic neck injuries(TNIs)constitute 5%–10%of all trauma cases.These injuries can be caused by either penetrating or blunt trauma.Patients can have a varied presentation like cut injury over the neck,bleeding,neck swelling,breathing difficulty,dysphagia,etc.Methods:This was a retrospective observational study conducted at a level I trauma center in India from January 2016 to March 2020.One hundred thirty patients who required admission and intervention due to TNIs were included in this study.Results:One hundred thirty patients with neck injuries were included in this study.Males were predominant(91.5%)with the age ranging from 10 to 70years.The most common mechanism of injury was physical assault(40.7%),followed by road traffic injury(23.8%)and self-inflicted injuries(18.4%).Penetrating trauma was predominant.Open neck wound with bleeding was the most common presenting symptom.Zone II injuries were more common(83.8%)followed by zone I(12.3%)and zone III(3.8%).Soft tissue injury including skin,platysma breach,and strap muscle injury was present in 46.9% of patients.Laryngopharyngeal injury was present in 13.8%,tracheal injury in 28.5%,vascular injury in 13.8%,and esophageal injury in 4.6% of patients.Conclusion:Penetrating neck trauma is more common than blunt in developing countries like India.Advanced Trauma Life Support(ATLS)protocol guides the initial management.The definitive management depends on the type and mechanism of injury,anatomical level,severity,and the organ injured.展开更多
文摘目的:探讨大鼠气管上皮损伤后,气管干细胞增殖分化过程中Shh信号通路的动态变化。方法:应用5-氟尿嘧啶(5-FU)诱发气管上皮损伤,通过RT-PCR检测气管上皮Shh的表达。结果:5-FU作用30 m in后大鼠气管上皮细胞绝大部分脱落,仅残留少量裸核样G0期细胞位于基底膜上。之后细胞数目逐渐增多,经过扁平、立方形态,向纤毛细胞、黏液细胞、基底细胞等分化,去除5-FU后48 h接近恢复假复层纤毛柱状上皮;正常气管上皮及5-FU作用30 m in气管上皮Shh无表达,去除5-FU恢复3 h后Shh轻度表达,6 h后表达增强,12 h达高峰,之后表达减弱,48 h已极微弱。结论:Shh参与了气管干细胞的增殖分化过程。
文摘Introduction:Traumatic neck injuries(TNIs)constitute 5%–10%of all trauma cases.These injuries can be caused by either penetrating or blunt trauma.Patients can have a varied presentation like cut injury over the neck,bleeding,neck swelling,breathing difficulty,dysphagia,etc.Methods:This was a retrospective observational study conducted at a level I trauma center in India from January 2016 to March 2020.One hundred thirty patients who required admission and intervention due to TNIs were included in this study.Results:One hundred thirty patients with neck injuries were included in this study.Males were predominant(91.5%)with the age ranging from 10 to 70years.The most common mechanism of injury was physical assault(40.7%),followed by road traffic injury(23.8%)and self-inflicted injuries(18.4%).Penetrating trauma was predominant.Open neck wound with bleeding was the most common presenting symptom.Zone II injuries were more common(83.8%)followed by zone I(12.3%)and zone III(3.8%).Soft tissue injury including skin,platysma breach,and strap muscle injury was present in 46.9% of patients.Laryngopharyngeal injury was present in 13.8%,tracheal injury in 28.5%,vascular injury in 13.8%,and esophageal injury in 4.6% of patients.Conclusion:Penetrating neck trauma is more common than blunt in developing countries like India.Advanced Trauma Life Support(ATLS)protocol guides the initial management.The definitive management depends on the type and mechanism of injury,anatomical level,severity,and the organ injured.
