摘要
目的探讨丙烯醛吸入损伤对大鼠气道脑源性神经营养素(BDNF)表达的影响。方法用丙烯醛雾化吸入造成成年雄性健康大鼠气道黏液高分泌损伤模型,损伤后不同时间点(1周、3周和6周各11只)取气管上皮,以生理盐水雾化吸入为对照(1、3、6周各11只)。用免疫组化和原位杂交染色检测BDNF蛋白和mRNA的定位分布,RT-PCR检测气道BDNF mRNA不同时程的表达变化。结果气管上皮和平滑肌可见BDNF阳性细胞,胞浆染色。RT-PCR显示与对照组相比,丙烯醛吸入1周气管中BDNF mRNA表达明显上调(P<0.05),随后逐渐回落,到第3周回到对照组水平,持续至第6周(P均>0.05)。结论丙烯醛吸入致大鼠气道损伤过程中,气管上皮和平滑肌BDNF表达早期明显上调,提示BDNF参与了气道损伤过程中神经源性炎性反应调控。
Objective To investigate expressional changes of brain derived neurotrophic factor（BDNF）in the trachea of rats with acrolein inhalation.Methods Twenty two SD rats were divided into 2groups：the rats in experimental group were subjected to acrolein inhalation for the induce of trachea inflammatory injury,while the rats with saline（NS）inhalation were as control.All the rats were sacrificed in 1,3,6weeks after acrolein（n=11at each time point）or saline inhalation（n=11at each time point）,the samples of trachea epithelium were harvested.The immunohistochemistry and in situ hybridization was performed to detect the location of BDNF protein and mRNA in trachea.The expression of BDNF mRNA in the trachea tissues were determined by RT-PCR.Results There are positive cells in epithelium of trachea for BDNF protein and mRNA,with cytoplasm staining.The expression of BDNF mRNA in the trachea was increased at 1week after acrolein inhalation（P〈 0.05,vs.control group）,then decreased along with the time and reached to the same level as control group at 3weeks,then last to 6weeks（P〉 0.05）.Conclusion The inflammatory injury in trachea induced by acrolein exposure could be associated with the increased expression of BDNF.BDNF may be one of the crucial inflammatory factors in the process of inflammatory reaction in trachea with acrolein stimulation.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2015年第4期560-563,共4页
Journal of Sichuan University(Medical Sciences)
基金
云南省自然科学基金(No.2011FZ272
No.2013FZ264)资助
