目的:探讨铒激光在口腔软硬组织中的临床效果及对消融率和应激反应的影响。方法:选择2018年3月~2020年1月口腔软硬组织患者94例作为研究对象,随机数字表法分为两组,各47例。对照组采用手术切除治疗,观察组采用铒激光治疗;于术后14 d评...目的:探讨铒激光在口腔软硬组织中的临床效果及对消融率和应激反应的影响。方法:选择2018年3月~2020年1月口腔软硬组织患者94例作为研究对象,随机数字表法分为两组,各47例。对照组采用手术切除治疗,观察组采用铒激光治疗;于术后14 d评估治疗效果并完成6个月随访,比较两组围术期指标、视觉模拟评分量表(VAS)评分、伤口愈合、手术时间、应激反应、消融率及安全性。结果:两组治疗前VAS评分、伤口愈合时间差异无统计学意义(P>0.05);观察组治疗时间、持续疼痛时间、治疗出血量及治疗后1 d、3 d VAS评分均短(低)于对照组(P<0.05);观察组治疗后14 d NO、Cor水平低于对照组,SOD水平高于对照组(P<0.05)。观察组治疗后1、3、6个月消融率均高于对照组(P<0.05)。观察组与对照组治疗过程中感染、创缘红肿及咽部疼痛发生率差异均无统计学意义。结论:铒激光用于口腔软硬组织中能创伤较小,能减轻疼痛,缩短手术及疼痛持续时间,降低应激发生,提高软硬组织消融率,且安全性较高。展开更多
Under some circumstances surgical resection is feasible in a low percentage for the treatment of deep tumors. Nevertheless, high-intensity focused ultrasound （HIFU） is beginning to offer a potential noninvasive alte...Under some circumstances surgical resection is feasible in a low percentage for the treatment of deep tumors. Nevertheless, high-intensity focused ultrasound （HIFU） is beginning to offer a potential noninvasive alternative to conventional therapies for the treatment of deep tumors. In our previous study, a large scale spherical HIFU-phased array was developed to ablate deep tumors. In the current study, taking into account the required focal depth and maximum acoustic power output, 90 identical circular PZT-8 elements （diameter=1.4 cm and frequency=l MHz） were mounted on a spherical shell with a radius of curvature of 18 cm and a diameter of 21 cm. With the developed array, computer simulations and ex vivo experiments were carried out. The simulation results theoretically demonstrate the ability of the array to focus and steer in the specified volume （a 2 cm×2 cm×3 cm volume） at the focal depth of 15 to 18 cm. Ex vivo experiment results also verify the capability of the developed array to ablate deep target tissue by either moving single focal point or generating multiple foci simultaneously.展开更多
Schwann cells are glial cells of peripheral nervous system, responsible for axonal myelination and ensheathing, as well as tissue repair following a peripheral nervous system injury. They are one of several cell types...Schwann cells are glial cells of peripheral nervous system, responsible for axonal myelination and ensheathing, as well as tissue repair following a peripheral nervous system injury. They are one of several cell types that are widely studied and most commonly used for cell transplantation to treat spinal cord injury, due to their intrinsic characteristics including the ability to secrete a variety of neurotrophic factors. This mini review summarizes the recent findings of endogenous Schwann cells after spinal cord injury and discusses their role in tissue repair and axonal regeneration. After spinal cord injury, numerous endogenous Schwann cells migrate into the lesion site from the nerve roots, involving in the construction of newly formed repaired tissue and axonal myelination. These invading Schwann cells also can move a long distance away from the injury site both rostrally and caudally. In addition, Schwann cells can be induced to migrate by minimal insults （such as scar ablation） within the spinal cord and integrate with astrocytes under certain circumstances. More importantly, the host Schwann cells can be induced to migrate into spinal cord by transplantation of different cell types, such as exogenous Schwann cells, olfactory ensheathing cells, and bone marrow-derived stromal stem cells. Migration of endogenous Schwann cells following spinal cord injury is a common natural phenomenon found both in animal and human, and the myelination by Schwann cells has been examined effective in signal conduction electrophysiologically. Therefore, if the inherent properties of endogenous Schwann cells could be developed and utilized, it would offer a new avenue for the restoration of injured spinal cord.展开更多
目的探讨心外膜脂肪组织（EAT）体积与心房颤动（AF）患者首次接受射频消融术后空白期复发的相关性。方法回顾性分析2014年1月至2016年5月收治的AF患者85例（AF组）及非AF患者90例（非AF组）的临床资料，均以螺旋CT检查对EAT体积进行测...目的探讨心外膜脂肪组织（EAT）体积与心房颤动（AF）患者首次接受射频消融术后空白期复发的相关性。方法回顾性分析2014年1月至2016年5月收治的AF患者85例（AF组）及非AF患者90例（非AF组）的临床资料，均以螺旋CT检查对EAT体积进行测量；术后随访6～18个月，以AF组术后空白期是否复发分为空白期AF复发组（观察组，27例）与空白期无AF复发组（对照组，58例），对比EAT体积在各组中的差异并进行相关性分析。结果观察组患者的总EAT体积[（118．71±28．94）cm^3 vs （97．73±24．86）cm^3]、左心房周EAT体积[（29．98±8．09）cm^3 vs（23．11±8．30）cm^3]显著高于对照组，差异有统计学意义（t=6．219、4．451，P〈0．01）。经多元Logistic回归分析提示，总EAT体积、左心房周EAT体积是独立预测AF发病的相关因素，也是独立预测射频}肖融术后空白期AF复发的因素；同时观察组患者的远期复发率[40．7％（11／27）]较对照组[15．5％（9／58）]更高，差异有统计学意义（X^2=7．142，P〈0．05）。结论当患者总EAT体积、左心房周EAT体积较大时，除了AF的发病率升高，首次接受射频消融术后空白期也更容易AF复发。展开更多
Alginate is a widely used hydrogel in tissue engineering owing to its simple and non-cytotoxic gelation process, ease of use, and abundance. However, unlike hydrogels derived from mammalian sources such as collagen, a...Alginate is a widely used hydrogel in tissue engineering owing to its simple and non-cytotoxic gelation process, ease of use, and abundance. However, unlike hydrogels derived from mammalian sources such as collagen, alginate does not contain cell adhesion Iigands. Here, we present a novel laser ablation technique for the in situ embedding of gold and iron nanoparticles into hydrogels. We hypothesized that integration of metal nanoparticles in alginate could serve as an alternative material because of its chemical biofunctionalization ability （coupling of RGD ligands） to favor cell adhesion. Cytocompatibility and biofunctionality of the gels were assessed by cell culture experiments using fibroblasts and endothelial cells. Nanoparticles with an average particle size of 3 nm （gold） and 6 nm （iron） were generated and stably maintained in alginate for up to 6 months. Using an extrusion system, several centimeter-long alginate tubes with an outer diameter of approximately 3 mm and a wall thickness of approximately 150 μm were manufactured. Confocal microscopy revealed homogeneously distributed nanoparticle agglomerates over the entire tube volume. Endothelial cells seeded on iron-loaded gels showed significantly higher viability and an increased degree of spreading, and the number of attached cells was also elevated in comparison to the control and gold-loaded alginates. We conclude that laser-based in situ integration of iron nanoparticles （40.01 wt.%） in alginate is a straightforward method to generate composite materials that favor the adhesion of endothelial cells. In addition, we show that nanoparticle integration does not impair the alginate＇s gelation and 3D biofabrication properties.展开更多
文摘目的:探讨铒激光在口腔软硬组织中的临床效果及对消融率和应激反应的影响。方法:选择2018年3月~2020年1月口腔软硬组织患者94例作为研究对象,随机数字表法分为两组,各47例。对照组采用手术切除治疗,观察组采用铒激光治疗;于术后14 d评估治疗效果并完成6个月随访,比较两组围术期指标、视觉模拟评分量表(VAS)评分、伤口愈合、手术时间、应激反应、消融率及安全性。结果:两组治疗前VAS评分、伤口愈合时间差异无统计学意义(P>0.05);观察组治疗时间、持续疼痛时间、治疗出血量及治疗后1 d、3 d VAS评分均短(低)于对照组(P<0.05);观察组治疗后14 d NO、Cor水平低于对照组,SOD水平高于对照组(P<0.05)。观察组治疗后1、3、6个月消融率均高于对照组(P<0.05)。观察组与对照组治疗过程中感染、创缘红肿及咽部疼痛发生率差异均无统计学意义。结论:铒激光用于口腔软硬组织中能创伤较小,能减轻疼痛,缩短手术及疼痛持续时间,降低应激发生,提高软硬组织消融率,且安全性较高。
基金supported by the National Natural Science Foundation of China (No. 30800246)Shanghai Key Technologies R&D Programof China (No. 09441900500)
文摘Under some circumstances surgical resection is feasible in a low percentage for the treatment of deep tumors. Nevertheless, high-intensity focused ultrasound （HIFU） is beginning to offer a potential noninvasive alternative to conventional therapies for the treatment of deep tumors. In our previous study, a large scale spherical HIFU-phased array was developed to ablate deep tumors. In the current study, taking into account the required focal depth and maximum acoustic power output, 90 identical circular PZT-8 elements （diameter=1.4 cm and frequency=l MHz） were mounted on a spherical shell with a radius of curvature of 18 cm and a diameter of 21 cm. With the developed array, computer simulations and ex vivo experiments were carried out. The simulation results theoretically demonstrate the ability of the array to focus and steer in the specified volume （a 2 cm×2 cm×3 cm volume） at the focal depth of 15 to 18 cm. Ex vivo experiment results also verify the capability of the developed array to ablate deep target tissue by either moving single focal point or generating multiple foci simultaneously.
文摘Schwann cells are glial cells of peripheral nervous system, responsible for axonal myelination and ensheathing, as well as tissue repair following a peripheral nervous system injury. They are one of several cell types that are widely studied and most commonly used for cell transplantation to treat spinal cord injury, due to their intrinsic characteristics including the ability to secrete a variety of neurotrophic factors. This mini review summarizes the recent findings of endogenous Schwann cells after spinal cord injury and discusses their role in tissue repair and axonal regeneration. After spinal cord injury, numerous endogenous Schwann cells migrate into the lesion site from the nerve roots, involving in the construction of newly formed repaired tissue and axonal myelination. These invading Schwann cells also can move a long distance away from the injury site both rostrally and caudally. In addition, Schwann cells can be induced to migrate by minimal insults （such as scar ablation） within the spinal cord and integrate with astrocytes under certain circumstances. More importantly, the host Schwann cells can be induced to migrate into spinal cord by transplantation of different cell types, such as exogenous Schwann cells, olfactory ensheathing cells, and bone marrow-derived stromal stem cells. Migration of endogenous Schwann cells following spinal cord injury is a common natural phenomenon found both in animal and human, and the myelination by Schwann cells has been examined effective in signal conduction electrophysiologically. Therefore, if the inherent properties of endogenous Schwann cells could be developed and utilized, it would offer a new avenue for the restoration of injured spinal cord.
文摘目的探讨心外膜脂肪组织（EAT）体积与心房颤动（AF）患者首次接受射频消融术后空白期复发的相关性。方法回顾性分析2014年1月至2016年5月收治的AF患者85例（AF组）及非AF患者90例（非AF组）的临床资料，均以螺旋CT检查对EAT体积进行测量；术后随访6～18个月，以AF组术后空白期是否复发分为空白期AF复发组（观察组，27例）与空白期无AF复发组（对照组，58例），对比EAT体积在各组中的差异并进行相关性分析。结果观察组患者的总EAT体积[（118．71±28．94）cm^3 vs （97．73±24．86）cm^3]、左心房周EAT体积[（29．98±8．09）cm^3 vs（23．11±8．30）cm^3]显著高于对照组，差异有统计学意义（t=6．219、4．451，P〈0．01）。经多元Logistic回归分析提示，总EAT体积、左心房周EAT体积是独立预测AF发病的相关因素，也是独立预测射频}肖融术后空白期AF复发的因素；同时观察组患者的远期复发率[40．7％（11／27）]较对照组[15．5％（9／58）]更高，差异有统计学意义（X^2=7．142，P〈0．05）。结论当患者总EAT体积、左心房周EAT体积较大时，除了AF的发病率升高，首次接受射频消融术后空白期也更容易AF复发。
文摘Alginate is a widely used hydrogel in tissue engineering owing to its simple and non-cytotoxic gelation process, ease of use, and abundance. However, unlike hydrogels derived from mammalian sources such as collagen, alginate does not contain cell adhesion Iigands. Here, we present a novel laser ablation technique for the in situ embedding of gold and iron nanoparticles into hydrogels. We hypothesized that integration of metal nanoparticles in alginate could serve as an alternative material because of its chemical biofunctionalization ability （coupling of RGD ligands） to favor cell adhesion. Cytocompatibility and biofunctionality of the gels were assessed by cell culture experiments using fibroblasts and endothelial cells. Nanoparticles with an average particle size of 3 nm （gold） and 6 nm （iron） were generated and stably maintained in alginate for up to 6 months. Using an extrusion system, several centimeter-long alginate tubes with an outer diameter of approximately 3 mm and a wall thickness of approximately 150 μm were manufactured. Confocal microscopy revealed homogeneously distributed nanoparticle agglomerates over the entire tube volume. Endothelial cells seeded on iron-loaded gels showed significantly higher viability and an increased degree of spreading, and the number of attached cells was also elevated in comparison to the control and gold-loaded alginates. We conclude that laser-based in situ integration of iron nanoparticles （40.01 wt.%） in alginate is a straightforward method to generate composite materials that favor the adhesion of endothelial cells. In addition, we show that nanoparticle integration does not impair the alginate＇s gelation and 3D biofabrication properties.
