Compound A was isolated from endophytic fungus( No.2526) of mangrove Avicennia from the South China Sea. The structure of compound A was elucidated by compr ehensive spectral data and coincided with that of sterigmato...Compound A was isolated from endophytic fungus( No.2526) of mangrove Avicennia from the South China Sea. The structure of compound A was elucidated by compr ehensive spectral data and coincided with that of sterigmatocystin. Compound A s howed a weak cytotoxic activity against Bel-7402 and NCIH-460 in vitro wit h IC 50 96 53×10 -6 and 72 52×10 -6 g/mL respectively. It is the first report on sterigmatocystin that isolated from marine fungus.展开更多
Objective To explore the effects of Sterigmatocystin (ST), Deoxynivalenol (DON) and Aflatoxin G1 (AFG1) on apoptosis of human peripheral blood lymphocytes (HPBLs) in vitro and thus to further elucidate the putative r...Objective To explore the effects of Sterigmatocystin (ST), Deoxynivalenol (DON) and Aflatoxin G1 (AFG1) on apoptosis of human peripheral blood lymphocytes (HPBLs) in vitro and thus to further elucidate the putative roles of these three mycotoxins on human immunosystem. Methods The effects of ST, DON and AFG1 on apoptosis of HPBLs were studied with cell culture, flow cytometric (FCM) DNA analysis and DNA agarose gel electrophoresis. Results DNA agarose gel electrophoresis results showed the characteristic 'ladder' pattern of apoptosis in HPBLs treated with ST, DON and AFG1. Flow cytometric DNA analysis revealed that typical subdiploid peaks of apoptosis in DNA histogram could be seen in all groups treated with the three mycotoxins. Significant time-effect and dose-effect relationships were found between the apoptosis rates and treatment time as well as concentrations of the three mycotoxins. Conclusion ST, DON and AFG1 can induce apoptosis of HPBLs in vitro and may have some negative effects on human immunosystem.展开更多
文摘Compound A was isolated from endophytic fungus( No.2526) of mangrove Avicennia from the South China Sea. The structure of compound A was elucidated by compr ehensive spectral data and coincided with that of sterigmatocystin. Compound A s howed a weak cytotoxic activity against Bel-7402 and NCIH-460 in vitro wit h IC 50 96 53×10 -6 and 72 52×10 -6 g/mL respectively. It is the first report on sterigmatocystin that isolated from marine fungus.
基金This work is supported by Natural Science Foundation of Hebei Province (No.3972537) A Grant from Ministry of Education of China for Young Teachers (HB-016).
文摘Objective To explore the effects of Sterigmatocystin (ST), Deoxynivalenol (DON) and Aflatoxin G1 (AFG1) on apoptosis of human peripheral blood lymphocytes (HPBLs) in vitro and thus to further elucidate the putative roles of these three mycotoxins on human immunosystem. Methods The effects of ST, DON and AFG1 on apoptosis of HPBLs were studied with cell culture, flow cytometric (FCM) DNA analysis and DNA agarose gel electrophoresis. Results DNA agarose gel electrophoresis results showed the characteristic 'ladder' pattern of apoptosis in HPBLs treated with ST, DON and AFG1. Flow cytometric DNA analysis revealed that typical subdiploid peaks of apoptosis in DNA histogram could be seen in all groups treated with the three mycotoxins. Significant time-effect and dose-effect relationships were found between the apoptosis rates and treatment time as well as concentrations of the three mycotoxins. Conclusion ST, DON and AFG1 can induce apoptosis of HPBLs in vitro and may have some negative effects on human immunosystem.
