Melatonin is a pleiotropic molecule that,after a short-term sleep deprivation,promotes the proliferation of neural stem cells in the adult hippocampus.However,this effect has not been observed in long-term sleep depri...Melatonin is a pleiotropic molecule that,after a short-term sleep deprivation,promotes the proliferation of neural stem cells in the adult hippocampus.However,this effect has not been observed in long-term sleep deprivation.The precise mechanism exerted by melatonin on the modulation of neural stem cells is not entirely elucidated,but evidence indicates that epigenetic regulators may be involved in this process.In this study,we investigated the effect of melatonin treatment during a 96-hour sleep deprivation and analyzed the expression of epigenetic modulators predicted by computational text mining and keyword clusterization.Our results showed that the administration of melatonin under sleep-deprived conditions increased the MECP2 expression and reduced the SIRT1 expression in the dentate gyrus.We observed that let-7 b,mir-132,and mir-124 were highly expressed in the dentate gyrus after melatonin administration,but they were not modified by sleep deprivation.In addition,we found more Sox2^+/5-bromo-2’-deoxyuridine(BrdU)^+cells in the subgranular zone of the sleep-deprived group treated with melatonin than in the untreated group.These findings may support the notion that melatonin modifies the expression of epigenetic mediators that,in turn,regulate the proliferation of neural progenitor cells in the adult dentate gyrus under long-term sleep-deprived conditions.All procedures performed in this study were approved by the Animal Ethics Committee of the University of Guadalajara,Mexico(approval No.CI-16610)on January 2,2016.展开更多
Objective: The objective is to observe the treatment effect of electro-acupuncture (EA) on core circadian clock gene Per2 and Bmal1 expression in hypothalamus of sleep-deprivation (SD) rats. Methods: Thirty-two Wistar...Objective: The objective is to observe the treatment effect of electro-acupuncture (EA) on core circadian clock gene Per2 and Bmal1 expression in hypothalamus of sleep-deprivation (SD) rats. Methods: Thirty-two Wistar male rats were randomly divided into 4 groups. Mice in the blank control group did not receive any treatment;the remaining groups were applied with para-chlorophenylalanine (PCPA) 300 mg/kg intraperitoneal injection for 2 days. Diazepam group received intraperitoneal injection of Diazepam (0.9 mg/kg, i.p.) one time a day for 5 days, while M group was treated with saline (0.9 mg/kg, i.p.) at the same time. Rats in EA group were given EA treatment, 20 minutes, once a day for 5 days, and rats in remaining groups were put into fixation-machine for the same time everyday, lasting for 5 days. Rats were sacrificed after anesthesia at the 8th day. Real-time PCR was adopted to detect the expression in clock gene Per2 and Bmal1 of each group. Results: Compared with blank control group, the expression of Per2 was significant decreased in PCPA model group (P 0.05). Conclusion: EA can significant up-regulate the expression of Per2 in SD rats, and down-regulate gene Bmal1 expression, and benefiting the weight of rats. Thus, EA is a potentially promising intervention to treat sleep-deprivation.展开更多
目的:观察酸枣仁总皂苷对老年阴血亏虚证失眠大鼠脑组织谷氨酸(Glu)、γ-氨基丁酸(GABA)及GABA A受体表达的影响。方法:将Wistar大鼠随机分为空白对照组(等容生理盐水),模型组(老年阴血亏虚证失眠组,等容生理盐水),阳性对照组(安定0.18 ...目的:观察酸枣仁总皂苷对老年阴血亏虚证失眠大鼠脑组织谷氨酸(Glu)、γ-氨基丁酸(GABA)及GABA A受体表达的影响。方法:将Wistar大鼠随机分为空白对照组(等容生理盐水),模型组(老年阴血亏虚证失眠组,等容生理盐水),阳性对照组(安定0.18 mg·kg-1·d-1),酸枣仁总皂苷低、高剂量组(15,30 g·kg-1·d-1)。用D-半乳糖致亚急性衰老、环磷酰胺及氢化可的松致阴血亏虚,及自制改良多平台法剥夺快动眼睡眠(REM)48 h,制作阴血亏虚证候失眠老年大鼠模型。造模后各组灌胃给药3周,采用高效液相法检测脑皮质、海马部位Glu,GABA含量;免疫组化染色及RT-PCR法检测脑皮质、海马部位GABA A R的表达变化,并考察酸枣仁总皂苷对这些指标的影响。结果:与空白对照组比较,模型组大鼠脑皮质及海马Glu,GABA含量显著升高(P<0.01,P<0.05),且Glu/GABA增高;脑皮质及海马部位的GABA A R免疫化学累积吸光度明显较高(P<0.01);皮质部位GABA A R mRNA的表达均显著上调(P<0.01),经酸枣仁总皂苷干预后各指标明显下降(P<0.01,P<0.05)。结论:酸枣仁总皂苷治疗阴血亏虚证老年失眠的机制可能与减少脑内氨基酸毒性作用,下调大脑皮质及海马部位GABA A R的表达有关。展开更多
基金supported by grants from Universidad de Guadalajara(PROSNI 2016,2017-8)to REGCpartially by grants from Consejo Nacional de Ciencia y Tecnologia(CONACyT No.PN 2016-01-465 and INFR-280414)+1 种基金PRODEP(213544)to OGPthe CONACyT Fellowship grant(374823)to AHG
文摘Melatonin is a pleiotropic molecule that,after a short-term sleep deprivation,promotes the proliferation of neural stem cells in the adult hippocampus.However,this effect has not been observed in long-term sleep deprivation.The precise mechanism exerted by melatonin on the modulation of neural stem cells is not entirely elucidated,but evidence indicates that epigenetic regulators may be involved in this process.In this study,we investigated the effect of melatonin treatment during a 96-hour sleep deprivation and analyzed the expression of epigenetic modulators predicted by computational text mining and keyword clusterization.Our results showed that the administration of melatonin under sleep-deprived conditions increased the MECP2 expression and reduced the SIRT1 expression in the dentate gyrus.We observed that let-7 b,mir-132,and mir-124 were highly expressed in the dentate gyrus after melatonin administration,but they were not modified by sleep deprivation.In addition,we found more Sox2^+/5-bromo-2’-deoxyuridine(BrdU)^+cells in the subgranular zone of the sleep-deprived group treated with melatonin than in the untreated group.These findings may support the notion that melatonin modifies the expression of epigenetic mediators that,in turn,regulate the proliferation of neural progenitor cells in the adult dentate gyrus under long-term sleep-deprived conditions.All procedures performed in this study were approved by the Animal Ethics Committee of the University of Guadalajara,Mexico(approval No.CI-16610)on January 2,2016.
文摘Objective: The objective is to observe the treatment effect of electro-acupuncture (EA) on core circadian clock gene Per2 and Bmal1 expression in hypothalamus of sleep-deprivation (SD) rats. Methods: Thirty-two Wistar male rats were randomly divided into 4 groups. Mice in the blank control group did not receive any treatment;the remaining groups were applied with para-chlorophenylalanine (PCPA) 300 mg/kg intraperitoneal injection for 2 days. Diazepam group received intraperitoneal injection of Diazepam (0.9 mg/kg, i.p.) one time a day for 5 days, while M group was treated with saline (0.9 mg/kg, i.p.) at the same time. Rats in EA group were given EA treatment, 20 minutes, once a day for 5 days, and rats in remaining groups were put into fixation-machine for the same time everyday, lasting for 5 days. Rats were sacrificed after anesthesia at the 8th day. Real-time PCR was adopted to detect the expression in clock gene Per2 and Bmal1 of each group. Results: Compared with blank control group, the expression of Per2 was significant decreased in PCPA model group (P 0.05). Conclusion: EA can significant up-regulate the expression of Per2 in SD rats, and down-regulate gene Bmal1 expression, and benefiting the weight of rats. Thus, EA is a potentially promising intervention to treat sleep-deprivation.
文摘目的:观察酸枣仁总皂苷对老年阴血亏虚证失眠大鼠脑组织谷氨酸(Glu)、γ-氨基丁酸(GABA)及GABA A受体表达的影响。方法:将Wistar大鼠随机分为空白对照组(等容生理盐水),模型组(老年阴血亏虚证失眠组,等容生理盐水),阳性对照组(安定0.18 mg·kg-1·d-1),酸枣仁总皂苷低、高剂量组(15,30 g·kg-1·d-1)。用D-半乳糖致亚急性衰老、环磷酰胺及氢化可的松致阴血亏虚,及自制改良多平台法剥夺快动眼睡眠(REM)48 h,制作阴血亏虚证候失眠老年大鼠模型。造模后各组灌胃给药3周,采用高效液相法检测脑皮质、海马部位Glu,GABA含量;免疫组化染色及RT-PCR法检测脑皮质、海马部位GABA A R的表达变化,并考察酸枣仁总皂苷对这些指标的影响。结果:与空白对照组比较,模型组大鼠脑皮质及海马Glu,GABA含量显著升高(P<0.01,P<0.05),且Glu/GABA增高;脑皮质及海马部位的GABA A R免疫化学累积吸光度明显较高(P<0.01);皮质部位GABA A R mRNA的表达均显著上调(P<0.01),经酸枣仁总皂苷干预后各指标明显下降(P<0.01,P<0.05)。结论:酸枣仁总皂苷治疗阴血亏虚证老年失眠的机制可能与减少脑内氨基酸毒性作用,下调大脑皮质及海马部位GABA A R的表达有关。
