An 86-year-old Japanese woman underwent an examining laparoscopy for removing the huge pelvic tumor. At laparoscope examination, the cystic tumor was found within the left broad ligament, while the ovaries, fallopian ...An 86-year-old Japanese woman underwent an examining laparoscopy for removing the huge pelvic tumor. At laparoscope examination, the cystic tumor was found within the left broad ligament, while the ovaries, fallopian tubes and uterus showed almost normal appearance. The tumor was removed together by total laparoscopic hysterectomy and bilateral salpingo-oophorectomies after the suction of serous content in the broad ligament. Cytological findings of the ascites suggested serous carcinoma. The resected ovaries and fallopian tubes were grossly and histologically normal. Histological examination of the solid part of broad ligament tumor, closely next to the fallopian tube, revealed a serous adenocarcinoma. Immunohistochemically, the tumor cells were strongly positive for CK7, WT-1, estrogen receptor, AE1/AE3 and EMA, and negative for CK20, D2-40 and calretinin. Also, they were negative for progesterone receptor and p53. The authors diagnosed the primary tumor as being a serous cystadenocarcinoma of the broad ligament [pTIC3NxM0, as modified and adapted to post-surgical staging of ovarian cancer (FIGO 2014)]. The patient has been receiving 6 cycles of adjuvant chemotherapies with one course with paclitaxel (PTX) and carboplatin (CBDCA) and five with PTX, CBDCA and Bevacizumab, and has no signs of recurrence and metastasis six months after the operation.展开更多
The human WFDC2 protein, also known as human epididymal secretory protein 4 （HE4）, is a new markerof tumor progressionJ that is used for the differential diagnosis of ovarian cystadenocarcinomas. At present, the pat...The human WFDC2 protein, also known as human epididymal secretory protein 4 （HE4）, is a new markerof tumor progressionJ that is used for the differential diagnosis of ovarian cystadenocarcinomas. At present, the pathology of ovarian carcinomas is defined by the “dualistic model”2 and “heterogeneity of ovarian carcinomas”3 theories. Previously, it was considered that ovarian carcinomas originate from the ovaries themselves. The ＂dualistic model＂ assumes an external origin for ovarian carcinomas, in which it is considered that serous ovarian carcinomas originate from secondary implantation of oviduct mucosa epithelium. Epithelial ovarian carcinomas are not a single disease, but a set of ＂heterogeneous diseases＂.3展开更多
目的探讨白细胞介素-17A(IL-17A)在卵巢癌组织中表达水平的变化及潜在机制。方法选取武警特色医学中心2018年1月至11月间收治的原发性卵巢浆液性囊腺癌患者20例,采用ELISA法检测肿瘤组织及癌旁正常组织IL-17A表达水平。下载肿瘤基因组图...目的探讨白细胞介素-17A(IL-17A)在卵巢癌组织中表达水平的变化及潜在机制。方法选取武警特色医学中心2018年1月至11月间收治的原发性卵巢浆液性囊腺癌患者20例,采用ELISA法检测肿瘤组织及癌旁正常组织IL-17A表达水平。下载肿瘤基因组图谱(TCGA)数据库中549例卵巢浆液性囊腺癌数据,按照IL-17A表达水平由高到低排序,将排在前50%的患者设为高表达组,剩余患者设为低表达组,绘制2组患者生存曲线;利用在线分析工具(http://www.linkedomics.org)将数据库中每一种基因的表达水平逐一与IL-17A表达水平进行Pearson相关性检测,根据相关系数选取100个相关基因,使用DAVID工具完成基因本体(GO)富集分析和京都基因与基因组百科全书通路(KEGG通路)富集分析。结果癌组织IL-17A表达水平显著高于癌旁正常组织([7.23±2.11)ng/L vs(4.19±1.33)ng/L,P<0.05];IL-17A高表达组中位生存时间高于IL-17A低表达组(1451 d vs 1348 d,风险比=0.74,95%CI为0.60~0.92,P<0.05);在检测的5309个基因中,有2103个基因与IL-17A存在相关性(P<0.05),其中钙电压门控通道亚单位α1(CACNA1S)与IL-17A表达水平相关系数最高(r=0.6928,P<0.05);GO富集分析和KEGG通路富集分析可见,相关基因主要涉及的生物学过程包括生物调节、刺激反应和多细胞生物过程,主要涉及的细胞组分为细胞膜、细胞核和囊泡,主要涉及的分子功能为蛋白质结合、离子结合和转运活性,主要涉及的信号通路为泛醌10和其他萜类醌的生物合成、ATP绑定蛋白转运和类固醇生物合成。结论IL-17A可作为评价卵巢浆液性囊腺癌预后的指标之一,这可能与其调控细胞膜表面电压门控通道及萜类醌的生物合成有关。展开更多
文摘An 86-year-old Japanese woman underwent an examining laparoscopy for removing the huge pelvic tumor. At laparoscope examination, the cystic tumor was found within the left broad ligament, while the ovaries, fallopian tubes and uterus showed almost normal appearance. The tumor was removed together by total laparoscopic hysterectomy and bilateral salpingo-oophorectomies after the suction of serous content in the broad ligament. Cytological findings of the ascites suggested serous carcinoma. The resected ovaries and fallopian tubes were grossly and histologically normal. Histological examination of the solid part of broad ligament tumor, closely next to the fallopian tube, revealed a serous adenocarcinoma. Immunohistochemically, the tumor cells were strongly positive for CK7, WT-1, estrogen receptor, AE1/AE3 and EMA, and negative for CK20, D2-40 and calretinin. Also, they were negative for progesterone receptor and p53. The authors diagnosed the primary tumor as being a serous cystadenocarcinoma of the broad ligament [pTIC3NxM0, as modified and adapted to post-surgical staging of ovarian cancer (FIGO 2014)]. The patient has been receiving 6 cycles of adjuvant chemotherapies with one course with paclitaxel (PTX) and carboplatin (CBDCA) and five with PTX, CBDCA and Bevacizumab, and has no signs of recurrence and metastasis six months after the operation.
文摘The human WFDC2 protein, also known as human epididymal secretory protein 4 （HE4）, is a new markerof tumor progressionJ that is used for the differential diagnosis of ovarian cystadenocarcinomas. At present, the pathology of ovarian carcinomas is defined by the “dualistic model”2 and “heterogeneity of ovarian carcinomas”3 theories. Previously, it was considered that ovarian carcinomas originate from the ovaries themselves. The ＂dualistic model＂ assumes an external origin for ovarian carcinomas, in which it is considered that serous ovarian carcinomas originate from secondary implantation of oviduct mucosa epithelium. Epithelial ovarian carcinomas are not a single disease, but a set of ＂heterogeneous diseases＂.3
文摘目的探讨白细胞介素-17A(IL-17A)在卵巢癌组织中表达水平的变化及潜在机制。方法选取武警特色医学中心2018年1月至11月间收治的原发性卵巢浆液性囊腺癌患者20例,采用ELISA法检测肿瘤组织及癌旁正常组织IL-17A表达水平。下载肿瘤基因组图谱(TCGA)数据库中549例卵巢浆液性囊腺癌数据,按照IL-17A表达水平由高到低排序,将排在前50%的患者设为高表达组,剩余患者设为低表达组,绘制2组患者生存曲线;利用在线分析工具(http://www.linkedomics.org)将数据库中每一种基因的表达水平逐一与IL-17A表达水平进行Pearson相关性检测,根据相关系数选取100个相关基因,使用DAVID工具完成基因本体(GO)富集分析和京都基因与基因组百科全书通路(KEGG通路)富集分析。结果癌组织IL-17A表达水平显著高于癌旁正常组织([7.23±2.11)ng/L vs(4.19±1.33)ng/L,P<0.05];IL-17A高表达组中位生存时间高于IL-17A低表达组(1451 d vs 1348 d,风险比=0.74,95%CI为0.60~0.92,P<0.05);在检测的5309个基因中,有2103个基因与IL-17A存在相关性(P<0.05),其中钙电压门控通道亚单位α1(CACNA1S)与IL-17A表达水平相关系数最高(r=0.6928,P<0.05);GO富集分析和KEGG通路富集分析可见,相关基因主要涉及的生物学过程包括生物调节、刺激反应和多细胞生物过程,主要涉及的细胞组分为细胞膜、细胞核和囊泡,主要涉及的分子功能为蛋白质结合、离子结合和转运活性,主要涉及的信号通路为泛醌10和其他萜类醌的生物合成、ATP绑定蛋白转运和类固醇生物合成。结论IL-17A可作为评价卵巢浆液性囊腺癌预后的指标之一,这可能与其调控细胞膜表面电压门控通道及萜类醌的生物合成有关。
