摘要
目的 研究卵巢浆液性囊腺癌(OSC)中长链非编码RNA泛素特异性蛋白酶30-反义RNA 1(USP30-AS1)的表达及其与免疫浸润的关系,并确定其在OSC中的预后作用。方法 通过癌症基因组图谱(TCGA)数据库检索了384名OSC患者的USP30-AS1表达和临床信息。Wilcoxon秩和检验用于比较OSC和正常卵巢组织中USP30-AS1的表达。采用逻辑回归分析临床病理特征与USP30-AS1的关系,进行基因集富集分析(GSEA)和单样本基因集富集分析(ssGSEA)以研究富集途径和功能,并量化USP30-AS1的免疫细胞浸润程度。根据长链非编码RNA (lncRNA) USP30-AS1的表达情况,根据表达均值将样本分为高表达组和低表达组。采用对数秩检验、单变量和多变量比例风险回归模型(Cox)用于比较不同USP30-AS1表达组之间的预后差异,lncRNA USP30-AS1的表达对其他基因组的影响也据此分析。结果 USP30-AS1高表达与肿瘤国际妇产科协会(FIGO)分期显著相关。多变量生存分析表明,USP30-AS1表达水平是OSC独立预后标志物。GSEA数据显示USP30-AS1高表达可能激活程序性细胞死亡蛋白1(PD-1)信号转导、细胞毒性T淋巴细胞相关蛋白4(CTLA4)通路、 B细胞受体信号通路、细胞凋亡、成纤维细胞生长因子受体(FGFR)信号通路,以及Janus激酶/信号转导子与转录激活子(JAK/STAT)信号通路。USP30-AS1表达与免疫细胞浸润呈负相关,包括B细胞、 CD4^(+)T细胞、树突状细胞、 CD8^(+)T细胞和中性粒细胞。结论 USP30-AS1有可能作为预测OSC预后的分子标志物。
Objective To investigate the expression of long non-coding RNA ubiquitin-specific peptidase 30 antisense RNA 1(IncRNA USP30-AS1)and its relationship with immune infiltration in ovarian serous cystadenocarcinoma(OSC),and to determine its prognostic role in OSC.Methods The Cancer Genome Atlas(TCGA)database was utilized to retrieve the expression of USP30-AS1 and clinical information of 384 OSC patients.Wilcoxon rank-sum test was employed to compare the expression of USP30-AS1 between OsC and normal ovarian tissues.Logistic regression analysis was conducted to assess the relationship between clinical pathological features and USP30-AS1.Gene set enrichment analysis(GSEA)and singlesample gene set enrichment analysis(ssGSEA)were performed to investigate enrichment pathways and functions and quantify the degree of immune cell infiltration in USP30-AS1.Based on the expression level of long non-coding RNA(IncRNA)USP30-AS1,the samples were divided into high and low expression groups according to the expression mean.Log-rank tests,univariate and multivariate proportional hazards model(Cox)were used to compare prognostic differences between different USP30-AS1 expression groups.The impact of IncRNA USP30-AS1 expression on other genomic analyses was also analyzed.Results High expression of USP30-AS1 was significantly associated with the International Federation of Gynecology and Obstetrics(FIGO)stage of the tumor.Multivariate survival analysis indicated that USP30-AS1 expression level served as an independent prognostic marker for OSC.GSEA data showed that high expression of USP30-AS1 might activate programmed death 1(PD-1)signaling pathway,cytotoxic T lymphocyte-associated protein 4(CTLA4)pathway,B-cell receptor signaling pathway,cell apoptosis,fibroblast growth factor receptor(FGFR)signaling pathway,and Janus kinase/signal transducer and activator of transcription(JAK/STAT)signaling pathway.The expression of USP30-AS1 was negatively correlated with immune cell infitration,including B cells,CD4^(+)T cells,dendritic cells,CD8
作者
王海燕
黄守国
蒙秋
张静
魏莉
WANG Haiyan;HUANG Shouguo;MENG Qiu;ZHANG Jing;WEI Li(Department of Obstetrics and Gynecology,Affiliated Haikou Hospital of Xiangya School of Medicine,Central South University,Haikou 570208;Department of Obstetrics and Gynecology,Xijing Hospital,Air Force Medical University,Xi'an 710032,China)
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2023年第9期834-840,共7页
Chinese Journal of Cellular and Molecular Immunology
基金
海南省自然科学基金(820QN424,821QN423)。
