A novel sensitive semi-quantitative virus detection technique was developed using the respiratory syncytial virus(RSV) as an example, through dark-field light scattering imaging of the surface state of the virusinvade...A novel sensitive semi-quantitative virus detection technique was developed using the respiratory syncytial virus(RSV) as an example, through dark-field light scattering imaging of the surface state of the virusinvaded host cells. In this method, anti-RSV-antibody modified gold nanoparticles(Au NPs) could bind with the invading virus on the cell membrane of the infected host cells through the specific antibody-antigen binding. Then,the host cells could be imaged by the localized surface plasmon resonance light scattering properties of Au NPs under a dark-field light scattering microscopy, which could be further used to semi-quantify the invading virus.展开更多
[目的]探究不同拉枝处理对葡萄冬芽成花过程中花发育相关基因表达的影响。[方法]试验以‘夏黑’葡萄(Vitis vinifera×V.labrusca‘Summer Black’)为试材,利用半定量RT-PCR技术研究不同拉枝处理对‘夏黑’葡萄长、中、短枝上的冬...[目的]探究不同拉枝处理对葡萄冬芽成花过程中花发育相关基因表达的影响。[方法]试验以‘夏黑’葡萄(Vitis vinifera×V.labrusca‘Summer Black’)为试材,利用半定量RT-PCR技术研究不同拉枝处理对‘夏黑’葡萄长、中、短枝上的冬芽成花过程中Vitis vinifera FLOWERING LOCUS T(VvFT)、Vitis vinifera SUPPRESSOR OF OVER EXPRESSION OF CO 1(VvSOC1)、Vitis vinifera LEAFY(VvLFY)、Vitis vinifera APETALA2(Vv AP2)和Vitis vinifera FLOWERING LOCUS C(VvFLC)5个重要花发育相关基因表达的影响进行了分析。[结果]不同拉枝处理间花发育相关基因的表达量存在差异,其中经平拉处理的基因表达量最高;同一拉枝处理中,长、中、短枝间基因的表达量也存在差异,且中、短枝上的表达量明显高于长枝。[结论]拉枝处理对促进‘夏黑’葡萄成花是有一定的影响的。展开更多
For the thorough research on coal metamorphism impact on gas adsorption capacity, this paper collected and summarized parameters of experimental adsorption isotherms, coal maceral, proximate analysis and ultimate anal...For the thorough research on coal metamorphism impact on gas adsorption capacity, this paper collected and summarized parameters of experimental adsorption isotherms, coal maceral, proximate analysis and ultimate analysis obtained from National Engineering Research Center of Coal Gas Control and related literatures at home and abroad, systematically discussed the coal rank effect on its physicochemical properties and methane adsorption capacity, in which the coal rank was shown in Vitrinite reflectance, furthermore, obtained the Semi-quantitative relationship between physicochemical properties of coal and methane adsorption capacity.展开更多
目的探索新加坡化学物质职业暴露半定量风险评估技术的适用性。方法应用新加坡化学物质职业暴露半定量风险评估模型对2家小型木质家具企业开展职业健康风险评估,并将评估结果与我国现行的职业接触限值和作业分级结果进行比较。结果 2家...目的探索新加坡化学物质职业暴露半定量风险评估技术的适用性。方法应用新加坡化学物质职业暴露半定量风险评估模型对2家小型木质家具企业开展职业健康风险评估,并将评估结果与我国现行的职业接触限值和作业分级结果进行比较。结果 2家企业主要生产工艺和岗位设置相近,主要职业病危害因素一致,现场均存在木粉尘超标,时间加权平均浓度(concentration of the time weighted average,CTWA)最高为29.42mg/m^3。用实际浓度计算暴露等级和用暴露指数计算的暴露等级获得的风险评估结果从低风险、中等风险、高风险到极高风险不等,且两种方法对同一岗位同一毒物的风险评估结果不一致,后者风险等级较高。结论应用半定量风险评估技术能预测和评估职业健康风险,但仍需开展更多的适用性研究来制定我国职业健康风险评估模型。展开更多
基金supported by the National Basic Research Program of China(2011CB933600)Chongqing Fundamental and Advanced Research Project(cstc2013jcyj A50008)the Fundamental Research Funds for the Central Universities(XDJK2015B029)
文摘A novel sensitive semi-quantitative virus detection technique was developed using the respiratory syncytial virus(RSV) as an example, through dark-field light scattering imaging of the surface state of the virusinvaded host cells. In this method, anti-RSV-antibody modified gold nanoparticles(Au NPs) could bind with the invading virus on the cell membrane of the infected host cells through the specific antibody-antigen binding. Then,the host cells could be imaged by the localized surface plasmon resonance light scattering properties of Au NPs under a dark-field light scattering microscopy, which could be further used to semi-quantify the invading virus.
文摘[目的]探究不同拉枝处理对葡萄冬芽成花过程中花发育相关基因表达的影响。[方法]试验以‘夏黑’葡萄(Vitis vinifera×V.labrusca‘Summer Black’)为试材,利用半定量RT-PCR技术研究不同拉枝处理对‘夏黑’葡萄长、中、短枝上的冬芽成花过程中Vitis vinifera FLOWERING LOCUS T(VvFT)、Vitis vinifera SUPPRESSOR OF OVER EXPRESSION OF CO 1(VvSOC1)、Vitis vinifera LEAFY(VvLFY)、Vitis vinifera APETALA2(Vv AP2)和Vitis vinifera FLOWERING LOCUS C(VvFLC)5个重要花发育相关基因表达的影响进行了分析。[结果]不同拉枝处理间花发育相关基因的表达量存在差异,其中经平拉处理的基因表达量最高;同一拉枝处理中,长、中、短枝间基因的表达量也存在差异,且中、短枝上的表达量明显高于长枝。[结论]拉枝处理对促进‘夏黑’葡萄成花是有一定的影响的。
文摘For the thorough research on coal metamorphism impact on gas adsorption capacity, this paper collected and summarized parameters of experimental adsorption isotherms, coal maceral, proximate analysis and ultimate analysis obtained from National Engineering Research Center of Coal Gas Control and related literatures at home and abroad, systematically discussed the coal rank effect on its physicochemical properties and methane adsorption capacity, in which the coal rank was shown in Vitrinite reflectance, furthermore, obtained the Semi-quantitative relationship between physicochemical properties of coal and methane adsorption capacity.
文摘目的探索新加坡化学物质职业暴露半定量风险评估技术的适用性。方法应用新加坡化学物质职业暴露半定量风险评估模型对2家小型木质家具企业开展职业健康风险评估,并将评估结果与我国现行的职业接触限值和作业分级结果进行比较。结果 2家企业主要生产工艺和岗位设置相近,主要职业病危害因素一致,现场均存在木粉尘超标,时间加权平均浓度(concentration of the time weighted average,CTWA)最高为29.42mg/m^3。用实际浓度计算暴露等级和用暴露指数计算的暴露等级获得的风险评估结果从低风险、中等风险、高风险到极高风险不等,且两种方法对同一岗位同一毒物的风险评估结果不一致,后者风险等级较高。结论应用半定量风险评估技术能预测和评估职业健康风险,但仍需开展更多的适用性研究来制定我国职业健康风险评估模型。
