摘要
RT-PCR是一种常用的具有很高灵敏度和特异性的基因表达检测方法。它有定量RT-PCR和半定量RT-PCR法。半定量RT-PCR法因其对试验条件的要求不高,费用低、普通实验室容易做到而被经常使用。本文从半定量RT-PCR的原理、技术、操作步骤及注意事进行了综述。
Reverse Transcription-Polymerase Chain Reaction （RT-PCR） is a highly sensitive and specific method useful for the detection of rare transcripts or for the analysis of samples available in limiting amounts. Hence, despite the greater accuracy of recently developed techniques, semi-quantitative methods are still widely used and appropriate for many puposes. Here we describe the principle, standard procedure and the necessary controls to ensure a quantitative analysis.
出处
《畜禽业》
2008年第2期34-37,共4页
Livestock and Poultry Industry
