The clearance of apoptotic cell debris,containing professional phagocytosis and non-professional phagocytosis,is essential for maintaining the homeostasis of healthy tissues.Here,we discovered that endothelial cells c...The clearance of apoptotic cell debris,containing professional phagocytosis and non-professional phagocytosis,is essential for maintaining the homeostasis of healthy tissues.Here,we discovered that endothelial cells could engulf apoptotic cell debris in atherosclerotic plaque.Single-cell RNA sequencing(RNA-seq)has revealed a unique endothelial cell subpopulation in atherosclerosis,which was strongly associated with vascular injury-related pathways.Moreover,integrated analysis of three vascular injury-related RNA-seq datasets showed that the expression of scavenger receptor class B type 1(SR-B1)was up-regulated and specifically enriched in the phagocytosis pathway under vascular injury circumstances.Single-cell RNA-seq and bulk RNA-seq indicate that SR-B1 was highly expressed in a unique endothelial cell subpopulation of mouse aorta and strongly associated with the reorganization of cellular adherent junctions and cytoskeleton which were necessary for phagocytosis.Furthermore,SR-B1 was strongly required for endothelial cells to engulf apoptotic cell debris in atherosclerotic plaque of both mouse and human aorta.Overall,this study demonstrated that apoptotic cell debris could be engulfed by endothelial cells through SR-B1 and associated with the reorganization of cellular adherent junctions and cytoskeleton.展开更多
The reverse cholesterol transport mediated by high-density lipoprotein (HDL) is an important mechanism for maintaining body cholesterol, and hence, the crucial anti-atherogenic action of the lipoprotein. Recent studie...The reverse cholesterol transport mediated by high-density lipoprotein (HDL) is an important mechanism for maintaining body cholesterol, and hence, the crucial anti-atherogenic action of the lipoprotein. Recent studies, however, have shown that HDL exerts a variety of anti-inflammatory and anti-atherogenic actions independently of cholesterol metabolism. The present review provides an overview of the roles of sphingosine 1-phosphate (S1P)/S1P receptor and apolipoprotein A-I/scavenger receptor class B type I systems in the anti-atherogenic HDL actions. In addition, the physiological significance of the existence of S1P in the HDL particles is discussed.展开更多
Class A scavenger receptor （SR-A） plays an important role in macrophage adhesion. However, the underlying mechanism remains unclear. We previously found that 78 kDa glucose-regulated protein （GRP78） inhibited SR- ...Class A scavenger receptor （SR-A） plays an important role in macrophage adhesion. However, the underlying mechanism remains unclear. We previously found that 78 kDa glucose-regulated protein （GRP78） inhibited SR- A-mediated ligand internalization into macrophage by binding to SR-A. The aim of the study was to investigate whether GRP78 could regulate SR-A-mediated cell adhesion. We demonstrated that GRP78 bound directly to SR-A by fluorescence resonance energy transfer （FRET） assay. Overexpression of GRP78 inhibited macrophage adhesion via SR-A. These results suggest that GRP78 may act as an inhibitor of macrophage adhesion via SR-A.展开更多
目的探讨A类Ⅰ型和Ⅱ型清道夫受体(scavenger receptor class A typesⅠandⅡ,SR-AⅠ/Ⅱ)基因缺失对高脂膳食小鼠脂质代谢的影响及其可能的作用机制。方法以SR-AⅠ/Ⅱ基因敲除与野生型雄性小鼠为对象,分别喂饲普通膳食和高脂膳食12w,应...目的探讨A类Ⅰ型和Ⅱ型清道夫受体(scavenger receptor class A typesⅠandⅡ,SR-AⅠ/Ⅱ)基因缺失对高脂膳食小鼠脂质代谢的影响及其可能的作用机制。方法以SR-AⅠ/Ⅱ基因敲除与野生型雄性小鼠为对象,分别喂饲普通膳食和高脂膳食12w,应用酶法或油红O染色法检测脂质代谢(包括血脂水平和肝脏脂质水平)的变化,采用RT-PCR法检测肝脏B类Ⅰ型清道夫受体(scavenger receptor class B typeⅠ,SR-BⅠ)和CD36的表达。结果SR-AⅠ/Ⅱ基因敲除鼠与野生型鼠相比,高脂膳食喂饲的第3、6、12w,其血清TG、TC、LDL、HDL均比野生型小鼠下降,肝细胞中脂滴的数量较多,体积较大,SR-BⅠmRNA表达上调,CD36mRNA的表达无差异。结论高脂膳食诱导SR-AⅠ/Ⅱ基因缺失小鼠脂质代谢的变化可能与肝脏SR-BⅠ表达升高及外周脂质向肝脏的逆向转运有关。展开更多
Lutein,a type of carotenoids,is found to delay the onset and progression of age-related macular degeneration(AMD).Several lutein supplementation studies showed that after an initial increase,lutein serum levels demons...Lutein,a type of carotenoids,is found to delay the onset and progression of age-related macular degeneration(AMD).Several lutein supplementation studies showed that after an initial increase,lutein serum levels demonstrated a subsequent decrease despite continuous supplementation.In this systematic literature review,this obscure phenomenon was tried to be explained.The subsequent drop in lutein levels was postulated due to down-regulation of lutein receptors scavenger receptor class B typeⅠ(SR-BI)in the gastrointestinal tract,upregulation of lutein degrading enzymeβ-carotene dioxygenase(BCDO2),or perhaps a combination of both.Some single nucleotides polymorphisms(SNPs)that could have influence on the occurrence of this phenomenon.To date,an exact scientific explanation for this phenomenon has not been established.Further research is needed to investigate this phenomenon in depth to reach an irrefutable explanation,giving that lutein is proven to be effective in delaying the onset and progression of AMD and its metabolism in the human body becomes of equal importance.展开更多
Stroke is a devastating disease that occurs when a blood vessel in the brain is either blocked or ruptured,consequently leading to deficits in neurological function.Stroke consistently ranked as one of the top causes ...Stroke is a devastating disease that occurs when a blood vessel in the brain is either blocked or ruptured,consequently leading to deficits in neurological function.Stroke consistently ranked as one of the top causes of mortality,and with the mean age of incidence decreasing,there is renewed interest to seek novel therapeutic treatments.The Scavenger Receptor Class B type 1(SR-B1)is a multifunctional protein found on the surface of a variety of cells.Research has found that that SR-B1 primarily functions in an anti-inflammatory and antiatherosclerotic capacity.In this review,we discuss the characteristics of SR-B1 and focus on its potential correlation with the modifiable risk factors of stroke.SR-B1 likely has an impact on stroke through its interaction with smoking,diabetes mellitus,diet,physical inactivity,obesity,hypercholesterolemia,atherosclerosis,coronary heart disease,hypertension,and sickle cell disease,all of which are critical risk factors in the pathogenesis of stroke.展开更多
清道夫受体A（scavenger receptor class A,SR-A）是一种以三聚体形式跨膜的糖蛋白受体,主要表达在单核细胞和巨噬细胞细胞膜。SR-A在细胞黏附、介导吞噬凋亡细胞、脂质代谢等方面发挥重要的生物学功能。最新研究发现SR-A通过启动机体...清道夫受体A（scavenger receptor class A,SR-A）是一种以三聚体形式跨膜的糖蛋白受体,主要表达在单核细胞和巨噬细胞细胞膜。SR-A在细胞黏附、介导吞噬凋亡细胞、脂质代谢等方面发挥重要的生物学功能。最新研究发现SR-A通过启动机体初始免疫进而在肺部疾病发生过程中发挥作用。本文就SR-A与肺部疾病关系作一综述。展开更多
基金the National Natural Science Foundation of China(No.12032007,31971242 to G.Wang)the Science and Technology Innovation Project of Jinfeng Laboratory,Chongqing,China(No.jfkyjf202203001 to G.Wang).
文摘The clearance of apoptotic cell debris,containing professional phagocytosis and non-professional phagocytosis,is essential for maintaining the homeostasis of healthy tissues.Here,we discovered that endothelial cells could engulf apoptotic cell debris in atherosclerotic plaque.Single-cell RNA sequencing(RNA-seq)has revealed a unique endothelial cell subpopulation in atherosclerosis,which was strongly associated with vascular injury-related pathways.Moreover,integrated analysis of three vascular injury-related RNA-seq datasets showed that the expression of scavenger receptor class B type 1(SR-B1)was up-regulated and specifically enriched in the phagocytosis pathway under vascular injury circumstances.Single-cell RNA-seq and bulk RNA-seq indicate that SR-B1 was highly expressed in a unique endothelial cell subpopulation of mouse aorta and strongly associated with the reorganization of cellular adherent junctions and cytoskeleton which were necessary for phagocytosis.Furthermore,SR-B1 was strongly required for endothelial cells to engulf apoptotic cell debris in atherosclerotic plaque of both mouse and human aorta.Overall,this study demonstrated that apoptotic cell debris could be engulfed by endothelial cells through SR-B1 and associated with the reorganization of cellular adherent junctions and cytoskeleton.
基金Supported by Grants-in-Aid for scientific research from the Japan Society for the Promotion of Science,No.20015008,20054003,and 21390016
文摘The reverse cholesterol transport mediated by high-density lipoprotein (HDL) is an important mechanism for maintaining body cholesterol, and hence, the crucial anti-atherogenic action of the lipoprotein. Recent studies, however, have shown that HDL exerts a variety of anti-inflammatory and anti-atherogenic actions independently of cholesterol metabolism. The present review provides an overview of the roles of sphingosine 1-phosphate (S1P)/S1P receptor and apolipoprotein A-I/scavenger receptor class B type I systems in the anti-atherogenic HDL actions. In addition, the physiological significance of the existence of S1P in the HDL particles is discussed.
基金supported by grants from the National Basic ResearchProgram(973)(No.2012CB517503,No.2011CB503903,and No.2012CB945003)National Natural Science Foundation of China(No.81070120)to Qi Chen+1 种基金National Natural Science Foundation ofChina(No.81000118)University Natural Science Foundation ofJiangsu(No.10KJB310005)to Jingjing Ben
文摘Class A scavenger receptor （SR-A） plays an important role in macrophage adhesion. However, the underlying mechanism remains unclear. We previously found that 78 kDa glucose-regulated protein （GRP78） inhibited SR- A-mediated ligand internalization into macrophage by binding to SR-A. The aim of the study was to investigate whether GRP78 could regulate SR-A-mediated cell adhesion. We demonstrated that GRP78 bound directly to SR-A by fluorescence resonance energy transfer （FRET） assay. Overexpression of GRP78 inhibited macrophage adhesion via SR-A. These results suggest that GRP78 may act as an inhibitor of macrophage adhesion via SR-A.
文摘目的探讨A类Ⅰ型和Ⅱ型清道夫受体(scavenger receptor class A typesⅠandⅡ,SR-AⅠ/Ⅱ)基因缺失对高脂膳食小鼠脂质代谢的影响及其可能的作用机制。方法以SR-AⅠ/Ⅱ基因敲除与野生型雄性小鼠为对象,分别喂饲普通膳食和高脂膳食12w,应用酶法或油红O染色法检测脂质代谢(包括血脂水平和肝脏脂质水平)的变化,采用RT-PCR法检测肝脏B类Ⅰ型清道夫受体(scavenger receptor class B typeⅠ,SR-BⅠ)和CD36的表达。结果SR-AⅠ/Ⅱ基因敲除鼠与野生型鼠相比,高脂膳食喂饲的第3、6、12w,其血清TG、TC、LDL、HDL均比野生型小鼠下降,肝细胞中脂滴的数量较多,体积较大,SR-BⅠmRNA表达上调,CD36mRNA的表达无差异。结论高脂膳食诱导SR-AⅠ/Ⅱ基因缺失小鼠脂质代谢的变化可能与肝脏SR-BⅠ表达升高及外周脂质向肝脏的逆向转运有关。
基金Supported by DSR Scholarship Support,King Saud University。
文摘Lutein,a type of carotenoids,is found to delay the onset and progression of age-related macular degeneration(AMD).Several lutein supplementation studies showed that after an initial increase,lutein serum levels demonstrated a subsequent decrease despite continuous supplementation.In this systematic literature review,this obscure phenomenon was tried to be explained.The subsequent drop in lutein levels was postulated due to down-regulation of lutein receptors scavenger receptor class B typeⅠ(SR-BI)in the gastrointestinal tract,upregulation of lutein degrading enzymeβ-carotene dioxygenase(BCDO2),or perhaps a combination of both.Some single nucleotides polymorphisms(SNPs)that could have influence on the occurrence of this phenomenon.To date,an exact scientific explanation for this phenomenon has not been established.Further research is needed to investigate this phenomenon in depth to reach an irrefutable explanation,giving that lutein is proven to be effective in delaying the onset and progression of AMD and its metabolism in the human body becomes of equal importance.
文摘Stroke is a devastating disease that occurs when a blood vessel in the brain is either blocked or ruptured,consequently leading to deficits in neurological function.Stroke consistently ranked as one of the top causes of mortality,and with the mean age of incidence decreasing,there is renewed interest to seek novel therapeutic treatments.The Scavenger Receptor Class B type 1(SR-B1)is a multifunctional protein found on the surface of a variety of cells.Research has found that that SR-B1 primarily functions in an anti-inflammatory and antiatherosclerotic capacity.In this review,we discuss the characteristics of SR-B1 and focus on its potential correlation with the modifiable risk factors of stroke.SR-B1 likely has an impact on stroke through its interaction with smoking,diabetes mellitus,diet,physical inactivity,obesity,hypercholesterolemia,atherosclerosis,coronary heart disease,hypertension,and sickle cell disease,all of which are critical risk factors in the pathogenesis of stroke.
文摘清道夫受体A（scavenger receptor class A,SR-A）是一种以三聚体形式跨膜的糖蛋白受体,主要表达在单核细胞和巨噬细胞细胞膜。SR-A在细胞黏附、介导吞噬凋亡细胞、脂质代谢等方面发挥重要的生物学功能。最新研究发现SR-A通过启动机体初始免疫进而在肺部疾病发生过程中发挥作用。本文就SR-A与肺部疾病关系作一综述。
