The article ＂Plasticity of human menstrual blood stem cells derived from the endometrium＂ by Lin et al （2011）, published in Journal of Zhejiang University- SCIENCE B （Biomedicine ＆ Biotechnology）, described a n...The article ＂Plasticity of human menstrual blood stem cells derived from the endometrium＂ by Lin et al （2011）, published in Journal of Zhejiang University- SCIENCE B （Biomedicine ＆ Biotechnology）, described a newly identified mesenchymal-like stem cell （MSC） from human menstrual blood known as MenSC. Here we describe the latest findings in this area and clarify the difference between human adult stem cells from menstrual blood and endometrial tissue.展开更多
Human induced pluripotent stem cells （hiPSCs） can self-renew indefinitely and have the potential to differentiate into all cell types in the human body, thus hold great promise in regen- erative medicine, drug scree...Human induced pluripotent stem cells （hiPSCs） can self-renew indefinitely and have the potential to differentiate into all cell types in the human body, thus hold great promise in regen- erative medicine, drug screening and developmental biology studies. However, integrating retroviral （Takahashi et al., 2007） or lentiviral （Hockemeyer et al., 2008） gene delivery systems and the exogenous oncogene c-Myc （Takahashi et al., 2007; Hockemeyer et al., 2008） have been typically used in the reprogramming process, which could increase risks of insertional mutations or epigenetic abnormalities and conse- quently pose biosecurity concerns for basic research and clinical applications of hiPSCs.展开更多
The endometrium is the inner mucosal lining of the uterus that undergoes extensive cyclic growth, regeneration, differentiation, and shedding throughout the menstrual cycle in response to steroid hormones. It repeated...The endometrium is the inner mucosal lining of the uterus that undergoes extensive cyclic growth, regeneration, differentiation, and shedding throughout the menstrual cycle in response to steroid hormones. It repeatedly undergoes approximately 450 cycles of degeneration and regeneration in a woman's lifetime. Endometrial abnormalities can be associated with repeated embryo implantation failure, recurrent spontaneous abortion, and other physiological features responsible for female infertility. This significant regenerative capacity may occur as a result of tissue-resident stem cell populations within the endometrium. Indeed, the existence of endometrial stem cells was only observed in humans and rodents through several isolation and characterization methods in the last few years. Although endometrial stem cells share various biological characteristics with other types of mesenchymal stem cells, they also show some differences in phenotype, self-renewal, and multilineage differentiation potential. Extensive studies over many years on endometrial stem cells will provide new insights into the physiology and mechanisms underlying various gynaecological diseases related to endometrial abnormalities such as female infertility, endometriosis, and endometrial cancer. Here we summarized recent studies about cellular origins and biological characteristics of endometrial stem cells. We also reviewed various recent studies to improve our understanding of their physiological roles. Many preclinical studies on their potential therapeutic applications to various endometrial diseases that could lead to reproductive dysfunction were also reviewed.展开更多
Recent advances in avian transgenic studies highlight the possibility of utilizing lentiviral vectors as tools to generate transgenic chickens. However, low rates of gonadal chimerism and germ line transmission effici...Recent advances in avian transgenic studies highlight the possibility of utilizing lentiviral vectors as tools to generate transgenic chickens. However, low rates of gonadal chimerism and germ line transmission efficiency still limit the broad usage of this method in creating transgenic chickens. In this study, we implemented a simple strategy using modified lentiviral vectors targeted to chicken primordial germ cells(PGCs) to generate transgenic chickens. The lentiviral vectors were pseudotyped with a modified Sindbis virus envelope protein(termed M168) and conjugated with an antibody specific to PGC membrane proteins. We demonstrated that these optimized M168-pseudotyped lentiviral vectors conjugated with SSEA4 antibodies successfully targeted transduction of PGCs in vitro and in vivo. Compared with the control, 50.0%–66.7% of chicken embryos expressed green fluorescent protein(GFP) in gonads transduced by the M168-pseudotyped lentivirus. This improved the targeted transduction efficiency by 30.0%–46.7%. Efficient chimerism of exogenous genes was also observed. This targeting technology could improve the efficiency of germ line transmission and provide greater opportunities for transgenic poultry studies.展开更多
文摘The article ＂Plasticity of human menstrual blood stem cells derived from the endometrium＂ by Lin et al （2011）, published in Journal of Zhejiang University- SCIENCE B （Biomedicine ＆ Biotechnology）, described a newly identified mesenchymal-like stem cell （MSC） from human menstrual blood known as MenSC. Here we describe the latest findings in this area and clarify the difference between human adult stem cells from menstrual blood and endometrial tissue.
基金supported by grants from the National Basic Research Program of China (No. 2013CB967102)the Strategic Priority Research Program of the Chinese Academy of Sciences (No. XDA01030506)the International S&T Cooperation Program of China (No. 2013DFG30680)
文摘Human induced pluripotent stem cells （hiPSCs） can self-renew indefinitely and have the potential to differentiate into all cell types in the human body, thus hold great promise in regen- erative medicine, drug screening and developmental biology studies. However, integrating retroviral （Takahashi et al., 2007） or lentiviral （Hockemeyer et al., 2008） gene delivery systems and the exogenous oncogene c-Myc （Takahashi et al., 2007; Hockemeyer et al., 2008） have been typically used in the reprogramming process, which could increase risks of insertional mutations or epigenetic abnormalities and conse- quently pose biosecurity concerns for basic research and clinical applications of hiPSCs.
基金supported by the Basic Science Research Program through the National Research Foundation of Korea(NRF)funded by the Ministry of Science,ICT and Future Planning(No.NRF-2021R1A2C2008424 and NRF-2021M3E5E5094127)This work was supported by the National Research Foundation of Korea(NRF)grant funded by the Korea government(MSIT)(No.NRF-2021R1A5A2030333)+3 种基金This work was supported by Korea Environment Industry&Technology Institute(KEITI)through the Project(Technology Development Project for Safety Management of Household Chemical Products)funded by Korea Ministry of Environment(MOE)(No.1485017593)This research was also supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute(KHIDI)funded by the Ministry of Health&Welfare,Republic of Korea(No HI21C1847).
文摘The endometrium is the inner mucosal lining of the uterus that undergoes extensive cyclic growth, regeneration, differentiation, and shedding throughout the menstrual cycle in response to steroid hormones. It repeatedly undergoes approximately 450 cycles of degeneration and regeneration in a woman's lifetime. Endometrial abnormalities can be associated with repeated embryo implantation failure, recurrent spontaneous abortion, and other physiological features responsible for female infertility. This significant regenerative capacity may occur as a result of tissue-resident stem cell populations within the endometrium. Indeed, the existence of endometrial stem cells was only observed in humans and rodents through several isolation and characterization methods in the last few years. Although endometrial stem cells share various biological characteristics with other types of mesenchymal stem cells, they also show some differences in phenotype, self-renewal, and multilineage differentiation potential. Extensive studies over many years on endometrial stem cells will provide new insights into the physiology and mechanisms underlying various gynaecological diseases related to endometrial abnormalities such as female infertility, endometriosis, and endometrial cancer. Here we summarized recent studies about cellular origins and biological characteristics of endometrial stem cells. We also reviewed various recent studies to improve our understanding of their physiological roles. Many preclinical studies on their potential therapeutic applications to various endometrial diseases that could lead to reproductive dysfunction were also reviewed.
基金the National Transgenic Breeding Project of China(2016ZX08009003006)National Natural Science Foundation of China(31672411)Discipline Innovative Engineering Plan(B12008)。
文摘Recent advances in avian transgenic studies highlight the possibility of utilizing lentiviral vectors as tools to generate transgenic chickens. However, low rates of gonadal chimerism and germ line transmission efficiency still limit the broad usage of this method in creating transgenic chickens. In this study, we implemented a simple strategy using modified lentiviral vectors targeted to chicken primordial germ cells(PGCs) to generate transgenic chickens. The lentiviral vectors were pseudotyped with a modified Sindbis virus envelope protein(termed M168) and conjugated with an antibody specific to PGC membrane proteins. We demonstrated that these optimized M168-pseudotyped lentiviral vectors conjugated with SSEA4 antibodies successfully targeted transduction of PGCs in vitro and in vivo. Compared with the control, 50.0%–66.7% of chicken embryos expressed green fluorescent protein(GFP) in gonads transduced by the M168-pseudotyped lentivirus. This improved the targeted transduction efficiency by 30.0%–46.7%. Efficient chimerism of exogenous genes was also observed. This targeting technology could improve the efficiency of germ line transmission and provide greater opportunities for transgenic poultry studies.
