Objective： To investigate the reversal effect of arsenic trioxide （As2O3） on the multidrug resistance of human gastric tumor SGC7901/ADR cell line to adriamycin （ADM） and its reversal mechanisms, Methods： The no...Objective： To investigate the reversal effect of arsenic trioxide （As2O3） on the multidrug resistance of human gastric tumor SGC7901/ADR cell line to adriamycin （ADM） and its reversal mechanisms, Methods： The non-cytotoxic concentration of As2O3 and the sensitivity of SGC7901/ADR cells toADM were detected by MTT assay, The drug concentration and P-gp function of SGC7901/ADR cells were measured with flow cytometry （FCM）, and the impacts of As2O3 on the GST-Π and Topo Ⅱ expressions of SGC7901/ADR cells were analyzed by immunohistochemical method. Results： As2O3 at 0,4 to 0.8 μmol/L concentrations were not significantly cytotoxic to SGC7901/ADR cells, As2O3 at 0.8 μmol/L could improve the sensitivity of SGC7901/ADR cells to ADM via inhibiting P-gp function, down-regulating GST-Π expression and increasing the intracellular accumulation of ADM in SGC7901/ADR cells. Conclusion： As2O3 can reverse partly the drug-resistance of SGC7901/ADR cells to ADM, which may be related with inhibiting the P-gp function and down-regulating GST-Π expression.展开更多
文摘Objective： To investigate the reversal effect of arsenic trioxide （As2O3） on the multidrug resistance of human gastric tumor SGC7901/ADR cell line to adriamycin （ADM） and its reversal mechanisms, Methods： The non-cytotoxic concentration of As2O3 and the sensitivity of SGC7901/ADR cells toADM were detected by MTT assay, The drug concentration and P-gp function of SGC7901/ADR cells were measured with flow cytometry （FCM）, and the impacts of As2O3 on the GST-Π and Topo Ⅱ expressions of SGC7901/ADR cells were analyzed by immunohistochemical method. Results： As2O3 at 0,4 to 0.8 μmol/L concentrations were not significantly cytotoxic to SGC7901/ADR cells, As2O3 at 0.8 μmol/L could improve the sensitivity of SGC7901/ADR cells to ADM via inhibiting P-gp function, down-regulating GST-Π expression and increasing the intracellular accumulation of ADM in SGC7901/ADR cells. Conclusion： As2O3 can reverse partly the drug-resistance of SGC7901/ADR cells to ADM, which may be related with inhibiting the P-gp function and down-regulating GST-Π expression.
