Background Yes-associated protein （YAP） plays an important role in signal transduction and gene transcription regulation in normal cells, with elevated and over-expressed YAP levels observed in various malignant tum...Background Yes-associated protein （YAP） plays an important role in signal transduction and gene transcription regulation in normal cells, with elevated and over-expressed YAP levels observed in various malignant tumors. The aim of this study was to investigate the expression of YAP in non-small cell lung cancer （NSCLC）, and to study the possible relationship of YAP expression with the occurrence and development of NSCLC. Methods YAP expression was assessed in 40 cases of NSCLC tumor tissues by immunohistochemistry, and their protein and mRNA levels were evaluated through Western blotting and reverse transcription-polymerase chain reaction （PCR）, respectively. Normal lung tissues obtained from the same patient were used as control. Statistical analysis was performed to correlate the YAP expression to clinical pathological factors, such as tumor type, stage and grade. Results YAP-positive expression was found in 28 （70%） of the 40 cases of NSCLC, which included 10 cases of squamous cell carcinoma （25%）, 17 cases of adenocarcinoma （42.5%） and 1 case of squamous adenocarcinoma （2.5%）. In the 28 YAP-positive cases, 19 cases showed lymph node metastasis and were classified in TNM stage II ＋ III （47.5%）; the other nine cases showed no lymph node metastasis （22.5%） and were classified in the TNM stage I. There was no relationship between YAP expression and patients＇ age, gender or tumor histological grades. However, YAP showed significant over expression in late period of T stage （P=0.012）, TNM stage （P=0.039）, and lymph node metastasis （P=-0.013）, respectively. Notably, YAP-positive expression was significantly higher in adenocarcinoma than that in squamous cell carcinoma （P=0.041）. Conclusions Over-expression of YAP was associated with NSCLC, especially lung adenocarcinoma. The high YAP expression in late period of tumor stage and lymph node metastasis may indicate that YAP expression could be an early marker for NSCLC tumorigenesis.展开更多
根据大蒜普通潜隐病毒(Garlic common latent virus,GCLV)的外壳蛋白区域的保守序列设计合成一对寡核苷酸引物,以带毒植物的总RNA为模板,进行反转录和PCR扩增,通过反应体系和反应程序的建立与优化,扩增得到长300 bp的目的片段,并将目的...根据大蒜普通潜隐病毒(Garlic common latent virus,GCLV)的外壳蛋白区域的保守序列设计合成一对寡核苷酸引物,以带毒植物的总RNA为模板,进行反转录和PCR扩增,通过反应体系和反应程序的建立与优化,扩增得到长300 bp的目的片段,并将目的片段转入大肠杆菌进行了克隆和序列测定。测序结果与GenBank中其他GCLV相应区域的序列同源性最高达98%。并对其检测的特异性和灵敏度进行了验证,从而建立了快速、灵敏、特异性强的GCLV分子生物学检测方法。展开更多
基金This study was supported by a grant from the Natural Science Foundation of Shandong Province,China
文摘Background Yes-associated protein （YAP） plays an important role in signal transduction and gene transcription regulation in normal cells, with elevated and over-expressed YAP levels observed in various malignant tumors. The aim of this study was to investigate the expression of YAP in non-small cell lung cancer （NSCLC）, and to study the possible relationship of YAP expression with the occurrence and development of NSCLC. Methods YAP expression was assessed in 40 cases of NSCLC tumor tissues by immunohistochemistry, and their protein and mRNA levels were evaluated through Western blotting and reverse transcription-polymerase chain reaction （PCR）, respectively. Normal lung tissues obtained from the same patient were used as control. Statistical analysis was performed to correlate the YAP expression to clinical pathological factors, such as tumor type, stage and grade. Results YAP-positive expression was found in 28 （70%） of the 40 cases of NSCLC, which included 10 cases of squamous cell carcinoma （25%）, 17 cases of adenocarcinoma （42.5%） and 1 case of squamous adenocarcinoma （2.5%）. In the 28 YAP-positive cases, 19 cases showed lymph node metastasis and were classified in TNM stage II ＋ III （47.5%）; the other nine cases showed no lymph node metastasis （22.5%） and were classified in the TNM stage I. There was no relationship between YAP expression and patients＇ age, gender or tumor histological grades. However, YAP showed significant over expression in late period of T stage （P=0.012）, TNM stage （P=0.039）, and lymph node metastasis （P=-0.013）, respectively. Notably, YAP-positive expression was significantly higher in adenocarcinoma than that in squamous cell carcinoma （P=0.041）. Conclusions Over-expression of YAP was associated with NSCLC, especially lung adenocarcinoma. The high YAP expression in late period of tumor stage and lymph node metastasis may indicate that YAP expression could be an early marker for NSCLC tumorigenesis.
文摘根据大蒜普通潜隐病毒(Garlic common latent virus,GCLV)的外壳蛋白区域的保守序列设计合成一对寡核苷酸引物,以带毒植物的总RNA为模板,进行反转录和PCR扩增,通过反应体系和反应程序的建立与优化,扩增得到长300 bp的目的片段,并将目的片段转入大肠杆菌进行了克隆和序列测定。测序结果与GenBank中其他GCLV相应区域的序列同源性最高达98%。并对其检测的特异性和灵敏度进行了验证,从而建立了快速、灵敏、特异性强的GCLV分子生物学检测方法。
