In acidic solution, thorin (2-(2-hydroxy-3.6-disulfo-1-naph-thyl)-azo-phenylarsenic acid sodium salt) can be bound with proteinand aggregated to form large particle which displays a very strong rayleigh light scatteri...In acidic solution, thorin (2-(2-hydroxy-3.6-disulfo-1-naph-thyl)-azo-phenylarsenic acid sodium salt) can be bound with proteinand aggregated to form large particle which displays a very strong rayleigh light scattering (RLS).The effects of pH, thorin concentration, detergent and ionic strength on binding reaction have beenstudied. The interference of coexisting substances was checked. The Scatchard plots for reactionbetween thorin and bovine serum albumin (BSA) were constructed and the association constant ofthorin-BSA was obtained, it is 5.26 x 10~5 L/mol, the maximum binding number is 7. RLS intensity iswell proportional to the concentrations of 2.0―14.0 μg/ mL for human serum albumin (HSA),1.8―14.7 μg/mL for BSA and 1.8―14.6 μg/mL for γ-globulin (γ-G). The detection limits (3σ) are54.1 ng/mL for HSA, 52.0 ng/mL for BSA and 51.8 ng/mL for γ-G, respectively. The relative standarddeviation is 2.4% for BSA, 3.2% for HSA and 4.1% for γ-G. The human serum samples were measuredsatisfactorily by using this method.展开更多
The recent applications of light scattering technique in biochemical analysis have been reviewed. The mechanic theories of resonance light scattering and Rayleigh light scattering (RLS), the relationships and differen...The recent applications of light scattering technique in biochemical analysis have been reviewed. The mechanic theories of resonance light scattering and Rayleigh light scattering (RLS), the relationships and differences between them, their applications and the interference factors in biochemistry analysis have been covered. By contrasting Rayleigh light scattering with double scattering and anti-scattering, the advantages of RLS have been pointed out. Finally, some prospects for the future application of this technique have been given.展开更多
A highly sensitive and simple Rayleigh light scattering （RLS） method for the determination of ciprofloxacin hydrochloride has been developed. Ciprofloxacin hydrochloride （CPFH） reacted with tetraphenylboron sodium...A highly sensitive and simple Rayleigh light scattering （RLS） method for the determination of ciprofloxacin hydrochloride has been developed. Ciprofloxacin hydrochloride （CPFH） reacted with tetraphenylboron sodium（TPB） to form an ion-association complex in pH 5.31 aqueous solution, which resulted in a significant enhancement of the RLS intensity, which was linear with the concentration of CPFH in the range of 12.7-419 ng·mL^-1. The limit of detection for CPFH was 6.99 ng·mL^-1. The method is simple, highly sensitive and selective within a wide linear range. Applying this method for the determination of ciprofloxacin hydrochloride in pharmaceutical preparations and biological fluids obtained satisfactory results.展开更多
文摘In acidic solution, thorin (2-(2-hydroxy-3.6-disulfo-1-naph-thyl)-azo-phenylarsenic acid sodium salt) can be bound with proteinand aggregated to form large particle which displays a very strong rayleigh light scattering (RLS).The effects of pH, thorin concentration, detergent and ionic strength on binding reaction have beenstudied. The interference of coexisting substances was checked. The Scatchard plots for reactionbetween thorin and bovine serum albumin (BSA) were constructed and the association constant ofthorin-BSA was obtained, it is 5.26 x 10~5 L/mol, the maximum binding number is 7. RLS intensity iswell proportional to the concentrations of 2.0―14.0 μg/ mL for human serum albumin (HSA),1.8―14.7 μg/mL for BSA and 1.8―14.6 μg/mL for γ-globulin (γ-G). The detection limits (3σ) are54.1 ng/mL for HSA, 52.0 ng/mL for BSA and 51.8 ng/mL for γ-G, respectively. The relative standarddeviation is 2.4% for BSA, 3.2% for HSA and 4.1% for γ-G. The human serum samples were measuredsatisfactorily by using this method.
文摘The recent applications of light scattering technique in biochemical analysis have been reviewed. The mechanic theories of resonance light scattering and Rayleigh light scattering (RLS), the relationships and differences between them, their applications and the interference factors in biochemistry analysis have been covered. By contrasting Rayleigh light scattering with double scattering and anti-scattering, the advantages of RLS have been pointed out. Finally, some prospects for the future application of this technique have been given.
基金Supported by the National Natural Science Foundation of China(20675059)Research Foundation of Leshan Teachers’ College, China (Z0625)
文摘A highly sensitive and simple Rayleigh light scattering （RLS） method for the determination of ciprofloxacin hydrochloride has been developed. Ciprofloxacin hydrochloride （CPFH） reacted with tetraphenylboron sodium（TPB） to form an ion-association complex in pH 5.31 aqueous solution, which resulted in a significant enhancement of the RLS intensity, which was linear with the concentration of CPFH in the range of 12.7-419 ng·mL^-1. The limit of detection for CPFH was 6.99 ng·mL^-1. The method is simple, highly sensitive and selective within a wide linear range. Applying this method for the determination of ciprofloxacin hydrochloride in pharmaceutical preparations and biological fluids obtained satisfactory results.
