摘要
样品(0~4.0mg)置于10mL比色管中,加入pH 7.64的三羟甲基氨基甲烷-盐酸缓冲溶液1.0mL和1.00×10-4 mol·L-1孔雀石绿溶液2.00mL,稀释至10mL,反应10min。呋塞米与孔雀石绿反应生成离子缔合物,使体系的瑞利光散射(RLS)发生猝灭作用,最大瑞利光散射峰位于370nm处。呋塞米的质量浓度在0.006~0.40mg·L-1范围内与对应的瑞利光散射猝灭程度(ΔIRLS)呈线性关系,检出限(3s/k)为0.005 4mg·L-1。按标准加入法进行回收试验,回收率为98.7%~102%,相对标准偏差(n=6)均小于3.0%。
The sample （0- 4. 0 mg） was placed into 10 mL colorimetric tube, and trihydroxymethyl aminomethane-HCl buffer solution of pH 7. 64 （1. 0 mL） and 1. 00×10^-4 mol·L^-1 malachite green solution （2.00 mL） were added. The obtained solution was diluted to 10 mL and reacted for 10 min. Furosemide reacted with malachite green to form an ionic association complexes, which led system＇s Rayleigh light scattering （RLS） had a quenched effect. The maximum RLS peak was located at 370 nm. Linear relationship between RLS quenched degree （Aims） and mass concentration of furosemide was found in the range of 0. 006 -0. 40 mg·L^-1 , with detection limit （3s/k） of O. 005 4 mg·L^-1. Test for recovery was made by standard addition method, giving results in the range of 98. 7%--102%, with RSDs （n=6） all less than 3. 0%.
作者
彭秋华
江虹
郑小敏
黄丹
于霞
王芳
PENG Qiuhua;JIANG Hong;ZHENG Xiaomin;HUANG Dan;YU Xia;WANG Fang(Collaborative Innovation Center for Green Development in Wuling Mountain Areas,College of Chemistry and Chemical Engineering,Yangtze Normal University,Chongqing 408100,China;Chongqing hechuan Dashi High School,Chongqing 401546,China;Chongqing Fuling Institute for Food and Drug Control,Chongqing 408000,China)
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2018年第7期792-795,共4页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
基金
重庆市教委科技基金资助项目(KJ1401226)
长江师范学院科技基金资助项目(2016cxx094)
关键词
呋塞米
孔雀石绿
药物
瑞利光散射
furosemide
malachite green
drug
Rayleigh light scattering
