Paper-based flexible surface-enhanced Raman scattering(SERS) chips have been demonstrated to have great potential for future practical applications in point-of-care testing(POCT) due to the potentials of massive fabri...Paper-based flexible surface-enhanced Raman scattering(SERS) chips have been demonstrated to have great potential for future practical applications in point-of-care testing(POCT) due to the potentials of massive fabrication, low cost, efficient sample collection and short signal acquisition time. In this work,common filter paper and Ag@Si O2 core-shell nanoparticles(NP) have been utilized to fabricate SERS chips based on shell-isolated nanoparticle-enhanced Raman spectroscopy(SHINERS). The SERS performance of the chips for POCT applications was systematically investigated. We used crystal violet as the model molecule to study the influence of the size of the Ag core and the thickness of the Si O2 coating layer on the SERS activity and then the morphology optimized Ag@Si O2 core-shell NPs was employed to detect thiram. By utilizing the smartphone as a miniaturized Raman spectral analyzer, high SERS sensitivity of thiram with a detection limit of 10^-9 M was obtained. The study on the stability of the SERS chips shows that a Si O2 shell of 3 nm can effectively protect the as-prepared SERS chips against oxidation in ambient atmosphere without seriously weakening the SERS sensitivity. Our results indicated that the SERS chips by SHINERS had great potential of practical application, such as pesticide residues detection in POCT.展开更多
目的探讨院外即时检测（Point of Care Test，POCT）肌钙蛋白I在院外急救中对急性心肌梗死（Acute Myocardial Infarction，AMI）的早期诊断价值。方法收集由本中心3个分站转送的“胸痛”疑似AMI患者在急救车上进行常规心电图（Electroc...目的探讨院外即时检测（Point of Care Test，POCT）肌钙蛋白I在院外急救中对急性心肌梗死（Acute Myocardial Infarction，AMI）的早期诊断价值。方法收集由本中心3个分站转送的“胸痛”疑似AMI患者在急救车上进行常规心电图（Electrocardiograph，ECG）检测及心肌肌钙蛋白I（cardiac TroponinI，cTnI）POCT检测，观察检测结果并与院内回访数据相结合，比较单项心电图检查、cTnI检测及二者联合检测的阳性检出率。结果院外心肌标志物cTnI即时定性检测比心电图法在院外急救“胸痛”病人中对AMI的诊断有明显较高的灵敏度（P=0．00028）。在医院确诊的31例AMI患者中，POCT法检测为24例（77％），心电图法检测为15例（48％）。结论在AMI患者的院外急救中引入院外及时检测肌钙蛋白可提高诊断率，缩短诊断时间，适合在院外快速筛选出AMI患者，对AMI早期诊断和再灌注治疗有重要价值，并适用于临床推广。展开更多
基金supported financially by the National Natural Science Foundation of China (No. 51802060)the Shenzhen Innovation Project (No. KQJSCX20170726104623185)the Shenzhen Peacock Group (KQTD20170809110344233)
文摘Paper-based flexible surface-enhanced Raman scattering(SERS) chips have been demonstrated to have great potential for future practical applications in point-of-care testing(POCT) due to the potentials of massive fabrication, low cost, efficient sample collection and short signal acquisition time. In this work,common filter paper and Ag@Si O2 core-shell nanoparticles(NP) have been utilized to fabricate SERS chips based on shell-isolated nanoparticle-enhanced Raman spectroscopy(SHINERS). The SERS performance of the chips for POCT applications was systematically investigated. We used crystal violet as the model molecule to study the influence of the size of the Ag core and the thickness of the Si O2 coating layer on the SERS activity and then the morphology optimized Ag@Si O2 core-shell NPs was employed to detect thiram. By utilizing the smartphone as a miniaturized Raman spectral analyzer, high SERS sensitivity of thiram with a detection limit of 10^-9 M was obtained. The study on the stability of the SERS chips shows that a Si O2 shell of 3 nm can effectively protect the as-prepared SERS chips against oxidation in ambient atmosphere without seriously weakening the SERS sensitivity. Our results indicated that the SERS chips by SHINERS had great potential of practical application, such as pesticide residues detection in POCT.
文摘目的探讨院外即时检测（Point of Care Test，POCT）肌钙蛋白I在院外急救中对急性心肌梗死（Acute Myocardial Infarction，AMI）的早期诊断价值。方法收集由本中心3个分站转送的“胸痛”疑似AMI患者在急救车上进行常规心电图（Electrocardiograph，ECG）检测及心肌肌钙蛋白I（cardiac TroponinI，cTnI）POCT检测，观察检测结果并与院内回访数据相结合，比较单项心电图检查、cTnI检测及二者联合检测的阳性检出率。结果院外心肌标志物cTnI即时定性检测比心电图法在院外急救“胸痛”病人中对AMI的诊断有明显较高的灵敏度（P=0．00028）。在医院确诊的31例AMI患者中，POCT法检测为24例（77％），心电图法检测为15例（48％）。结论在AMI患者的院外急救中引入院外及时检测肌钙蛋白可提高诊断率，缩短诊断时间，适合在院外快速筛选出AMI患者，对AMI早期诊断和再灌注治疗有重要价值，并适用于临床推广。
