目的探讨芪黄煎剂对大鼠胃切除后Peyer结淋巴细胞的影响。方法将90只大鼠随机分为假手术组、模型组、芪黄煎剂组,每组30只。假手术组大鼠仅给予腹部正中切开后缝合,不行胃切除,手术后自由饮水进食,不给予肠内营养和芪黄煎剂;模型组大鼠...目的探讨芪黄煎剂对大鼠胃切除后Peyer结淋巴细胞的影响。方法将90只大鼠随机分为假手术组、模型组、芪黄煎剂组,每组30只。假手术组大鼠仅给予腹部正中切开后缝合,不行胃切除,手术后自由饮水进食,不给予肠内营养和芪黄煎剂;模型组大鼠行胃切除手术后给予肠内营养制剂能全素;芪黄煎剂组大鼠行胃切除手术后给予能全素和芪黄煎剂。疗程1周。疗程结束后,处死大鼠,分离出Peyer结及其内淋巴细胞,采用流式细胞仪检测αβT细胞抗原受体-白细胞分化抗原3阳性(αβT cell antigen receptor-cluster of differentia-tion 3 positive,αβTCR-CD3+)T细胞,白细胞分化抗原4阳性(cluster of differentiation 4 positive,CD4+)、白细胞分化抗原8阳性(cluster of differentiation 8 positive,CD8+)T细胞;免疫组织化学法检测免疫球蛋白A阳性(immunoglobulin A positive,IgA+)B细胞。结果模型复制后1周,与假手术组比较,模型组Peyer结中αβTCR-CD3+、CD4+T细胞构成比和IgA+B细胞数显著降低(P<0.05,或P<0.01),模型组和芪黄煎剂组CD8+T细胞构成比无显著变化;与模型组比较,芪黄煎剂组Peyer结中αβTCR-CD3+、CD4+T细胞构成比和IgA+B细胞数显著上升(P<0.05,或P<0.01)。结论芪黄煎剂能刺激Peyer结中T细胞的成熟、分化和增殖,并进一步促进Peyer结中B细胞的增殖和活化,有利于胃切除手术应激后肠道免疫屏障功能的调节和恢复。展开更多
The interaction between the receptor activator of NF-κB ligand(RANKL)and its receptor RANK plays a critical role in the development and function of diverse tissues.This review summarizes the studies regarding the fun...The interaction between the receptor activator of NF-κB ligand(RANKL)and its receptor RANK plays a critical role in the development and function of diverse tissues.This review summarizes the studies regarding the functions of RANKL signaling in immune regulatory systems.Previous in vitro and in vivo studies have indicated that the RANKL signal promotes the survival of dendritic cells(DCs),thereby activating the immune response.In addition,RANKL signaling to DCs in the body surface barriers controls self-tolerance and oral-tolerance through regulatory T cell functions.In addition to regulating DC functions,the RANKL and RANK interaction is critical for the development and organization of several lymphoid organs.The RANKL signal initiates the formation of clusters of lymphoid tissue inducer cells,which is crucial for lymph node organogenesis.Moreover,the RANKL-RANK interaction controls the differentiation of M cells,specialized epithelial cells in mucosal tissues,that take up and transcytose antigen particles to control the immune response to pathogens or commensal bacterium.The development of epithelial cells localized in the thymic medulla(m TECs)is also regulated by the RANKL-RANK signal.Given that the unique property ofm TECs to express a wide variety of tissue-specific selfantigens is critical for the elimination of self-antigen reactive T cells in the thymus,the RANKL-RANK interaction contributes to the suppression of autoimmunity.Future studies on the roles of the RANKL-RANK system in immune regulatory functions would be informative for the development and application of inhibitors of RANKL signaling for disease treatment.展开更多
目的探讨Peyer小结在IgA肾病(IgAN)发病中的作用。方法雄性BALB/C小鼠予牛血清清蛋白、葡萄球菌肠毒素B诱导IgAN模型,另选40只正常雄性BALB/C小鼠作为对照组。第12周免疫荧光检测其肾组织IgA沉积,测定其血清总蛋白(TP)、清蛋白(Alb)、BU...目的探讨Peyer小结在IgA肾病(IgAN)发病中的作用。方法雄性BALB/C小鼠予牛血清清蛋白、葡萄球菌肠毒素B诱导IgAN模型,另选40只正常雄性BALB/C小鼠作为对照组。第12周免疫荧光检测其肾组织IgA沉积,测定其血清总蛋白(TP)、清蛋白(Alb)、BUN、Cr、半胱氨酸蛋白酶抑制剂C(Cys C)。荧光定量反转录(RT)-PCR检测Peyer小结IL-21、转化生长因子-β1(TGF-β1)mRNA的表达。Western blot测定Peyer小结IL-21、B细胞淋巴瘤因子-6(Bcl-6)、B淋巴细胞诱导成熟蛋白-1(Blimp-1)蛋白表达。结果 1.IgAN组和对照组第0周、第6周、第12周24 h尿蛋白定量分别为(0.61±0.26)mg vs(0.67±0.22)mg、(3.61±0.75)mg vs(0.77±0.27)mg、(4.15±0.82)mg vs(2.09±0.51)mg,IgAN组第6周、第12周24 h尿蛋白均高于对照组(Pa<0.01)。2.IgAN组和对照组IL-21、TGF-β1 mRNA表达相对值分别为1.67±0.13 vs 1.48±0.13、1.21±0.09 vs 1.10±0.10,与对照组相比,IgAN组IL-21、TGF-β1 mRNA表达均增加,差异均有统计学意义(Pa<0.05)。3.Western blot显示IgAN组和对照组IL-21、Bcl-6、Blimp-1蛋白表达相对值分别为0.67±0.21 vs 0.45±0.10、0.34±0.21 vs 0.60±0.19、1.03±0.07 vs 0.67±0.07,与对照组相比,IgAN组IL-21、Blimp-1蛋白表达均显著增高,差异均有统计学意义(Pa<0.05),Bcl-6蛋白表达降低,差异有统计学意义(P<0.05)。结论 Peyer小结有促进B淋巴细胞向分泌IgA浆母细胞分化的微环境,参与IgAN的发病。展开更多
文摘目的探讨芪黄煎剂对大鼠胃切除后Peyer结淋巴细胞的影响。方法将90只大鼠随机分为假手术组、模型组、芪黄煎剂组,每组30只。假手术组大鼠仅给予腹部正中切开后缝合,不行胃切除,手术后自由饮水进食,不给予肠内营养和芪黄煎剂;模型组大鼠行胃切除手术后给予肠内营养制剂能全素;芪黄煎剂组大鼠行胃切除手术后给予能全素和芪黄煎剂。疗程1周。疗程结束后,处死大鼠,分离出Peyer结及其内淋巴细胞,采用流式细胞仪检测αβT细胞抗原受体-白细胞分化抗原3阳性(αβT cell antigen receptor-cluster of differentia-tion 3 positive,αβTCR-CD3+)T细胞,白细胞分化抗原4阳性(cluster of differentiation 4 positive,CD4+)、白细胞分化抗原8阳性(cluster of differentiation 8 positive,CD8+)T细胞;免疫组织化学法检测免疫球蛋白A阳性(immunoglobulin A positive,IgA+)B细胞。结果模型复制后1周,与假手术组比较,模型组Peyer结中αβTCR-CD3+、CD4+T细胞构成比和IgA+B细胞数显著降低(P<0.05,或P<0.01),模型组和芪黄煎剂组CD8+T细胞构成比无显著变化;与模型组比较,芪黄煎剂组Peyer结中αβTCR-CD3+、CD4+T细胞构成比和IgA+B细胞数显著上升(P<0.05,或P<0.01)。结论芪黄煎剂能刺激Peyer结中T细胞的成熟、分化和增殖,并进一步促进Peyer结中B细胞的增殖和活化,有利于胃切除手术应激后肠道免疫屏障功能的调节和恢复。
文摘The interaction between the receptor activator of NF-κB ligand(RANKL)and its receptor RANK plays a critical role in the development and function of diverse tissues.This review summarizes the studies regarding the functions of RANKL signaling in immune regulatory systems.Previous in vitro and in vivo studies have indicated that the RANKL signal promotes the survival of dendritic cells(DCs),thereby activating the immune response.In addition,RANKL signaling to DCs in the body surface barriers controls self-tolerance and oral-tolerance through regulatory T cell functions.In addition to regulating DC functions,the RANKL and RANK interaction is critical for the development and organization of several lymphoid organs.The RANKL signal initiates the formation of clusters of lymphoid tissue inducer cells,which is crucial for lymph node organogenesis.Moreover,the RANKL-RANK interaction controls the differentiation of M cells,specialized epithelial cells in mucosal tissues,that take up and transcytose antigen particles to control the immune response to pathogens or commensal bacterium.The development of epithelial cells localized in the thymic medulla(m TECs)is also regulated by the RANKL-RANK signal.Given that the unique property ofm TECs to express a wide variety of tissue-specific selfantigens is critical for the elimination of self-antigen reactive T cells in the thymus,the RANKL-RANK interaction contributes to the suppression of autoimmunity.Future studies on the roles of the RANKL-RANK system in immune regulatory functions would be informative for the development and application of inhibitors of RANKL signaling for disease treatment.
文摘目的探讨Peyer小结在IgA肾病(IgAN)发病中的作用。方法雄性BALB/C小鼠予牛血清清蛋白、葡萄球菌肠毒素B诱导IgAN模型,另选40只正常雄性BALB/C小鼠作为对照组。第12周免疫荧光检测其肾组织IgA沉积,测定其血清总蛋白(TP)、清蛋白(Alb)、BUN、Cr、半胱氨酸蛋白酶抑制剂C(Cys C)。荧光定量反转录(RT)-PCR检测Peyer小结IL-21、转化生长因子-β1(TGF-β1)mRNA的表达。Western blot测定Peyer小结IL-21、B细胞淋巴瘤因子-6(Bcl-6)、B淋巴细胞诱导成熟蛋白-1(Blimp-1)蛋白表达。结果 1.IgAN组和对照组第0周、第6周、第12周24 h尿蛋白定量分别为(0.61±0.26)mg vs(0.67±0.22)mg、(3.61±0.75)mg vs(0.77±0.27)mg、(4.15±0.82)mg vs(2.09±0.51)mg,IgAN组第6周、第12周24 h尿蛋白均高于对照组(Pa<0.01)。2.IgAN组和对照组IL-21、TGF-β1 mRNA表达相对值分别为1.67±0.13 vs 1.48±0.13、1.21±0.09 vs 1.10±0.10,与对照组相比,IgAN组IL-21、TGF-β1 mRNA表达均增加,差异均有统计学意义(Pa<0.05)。3.Western blot显示IgAN组和对照组IL-21、Bcl-6、Blimp-1蛋白表达相对值分别为0.67±0.21 vs 0.45±0.10、0.34±0.21 vs 0.60±0.19、1.03±0.07 vs 0.67±0.07,与对照组相比,IgAN组IL-21、Blimp-1蛋白表达均显著增高,差异均有统计学意义(Pa<0.05),Bcl-6蛋白表达降低,差异有统计学意义(P<0.05)。结论 Peyer小结有促进B淋巴细胞向分泌IgA浆母细胞分化的微环境,参与IgAN的发病。
