摘要
目的 研究经口感染弓形虫对小鼠免疫器官的影响。方法 两组小鼠分别灌胃接种含 5× 10 4个速殖子的液体 0 .5ml或相同剂量的PBS ,观察小鼠的体况、胸腺、脾、Peyer’spatches(PP结 )的变化。 结果 经口接种弓形虫速殖子可致小鼠感染 ,在第 8,10天表现最为严重。胸腺重量略有减轻 ;PP结肿大 ,但数目变化不显著 ;脾脏增重 ,尤其在第 8,10天与对照组相比差异显著 (P <0 .0 1)。结论 5× 10 4个弓形虫速殖子灌胃接种可使小鼠出现一过性临床症状 ,并可以诱导机体黏膜和系统免疫应答 ,使免疫器官发生病理改变。
Objective To study the effects of oral infection of Toxoplasma gondii on immune organs in mice. Methods There were two groups of mice (each n=50) . Test group was given orally 5×10 4 tachyzoites of RH strain in each 0.5ml, while the control group was given equal volume of PBS, observing the symptoms of mice. The thymus and spleen were weighed and the numbers of the Peyer's patches (PP) was counted. Results Inoculation tachyzoites of Toxoplasma gondii induced mouse infection. Symptoms were most serious on d 8 and 10 post-infection. The thymus weight decreased and spleen weight increased. The PP showedno noticeable change in the number, but it slightly enlarged. There was high significant difference on d 8 and 10 post-infection between both groups (P<001). Conclusion The mice which have been orally infected 5×10 4 tachyzoites show transient symptoms. During oral infection of Toxoplasma gondii both systemic and mucosal immune responses are induced, so the pathological changes appear.
出处
《山西医科大学学报》
CAS
2004年第4期347-349,共3页
Journal of Shanxi Medical University
基金
山西省自然科学基金资助项目 ( 2 0 0 4110 5 )
