The sorption and desorption behaviors of two perfluoroalkane sulfonates（PFSAs）, including perfluorohexane sulfonate（PFHx S） and perfluorooctane sulfonate（PFOS） on two humic acids（HAs） and humin（HM）, which we...The sorption and desorption behaviors of two perfluoroalkane sulfonates（PFSAs）, including perfluorohexane sulfonate（PFHx S） and perfluorooctane sulfonate（PFOS） on two humic acids（HAs） and humin（HM）, which were extracted from a peat soil, were investigated. The sorption kinetics and isotherms showed that the sorption of PFOS on the humic substances（HSs） was much higher than PFHx S. For the same PFSA compound, the sorption on HSs followed the order of HM 〉 HA2 〉 HA1. These suggest that hydrophobic interaction plays a key role in the sorption of PFSAs on HSs. The sorption capacities of PFSAs on HSs were significantly related to their aliphaticity, but negatively correlated to aromatic carbons,indicating the importance of aliphatic groups in the sorption of PFSAs. Compared to PFOS,PFHx S displayed distinct desorption hysteresis, probably due to irreversible pore deformation after sorption of PFHx S. The sorption of the two PFSAs on HSs decreased with an increase in p H in the solution. This is ascribed to the electrostatic interaction and hydrogen bonding at lower p H. Hydrophobic interaction might also be stronger at lower p H due to the aggregation of HSs.展开更多
Hypoxia,an important characteristic of bacterial biofilms,can hinder the generation of reactive oxygen species(ROS)in photodynamic therapy(PDT),leading to reduced therapeutic efficacy of PDT.In order to address this i...Hypoxia,an important characteristic of bacterial biofilms,can hinder the generation of reactive oxygen species(ROS)in photodynamic therapy(PDT),leading to reduced therapeutic efficacy of PDT.In order to address this issue,fluorinated liposome was fabricated as an oxygen-sufficient nanoplatform for enhanced photodynamic eradication of bacterial biofilms.The liposomes(denoted as Lip-Ce6-PFH@O_(2))were prepared by co-encapsulation of O_(2) carrier perfluorohexane(PFH)and photosensitizer chlorin e6(Ce_(6)).Lip-Ce6-PFH@O_(2) could achieve efficient biofilm penetration due to the positively charged surface.The hypoxic microenvironment of biofilms would then be relieved,leading to the generation of more ROS under laser irradiation.Therefore,the bactericidal capability of PDT could be significantly improved because of the co-delivered O_(2) carrier PFH.Lip-Ce6-PFH@O_(2) exhibited much better antibiofilm ability than that of Lip-Ce6 both in vitro and in vivo.Meanwhile,Lip-Ce6-PFH@O_(2) also effectively alleviated inflammation symptoms and accelerated wound healing in the mice model.In general,this study provides a new paradigm to enhance the therapeutic efficacy of PDT for efficient biofilm eradication.展开更多
目的制备共载光敏剂二氢卟吩(Ce6)和氧载体全氟己烷(PFH)的长循环脂质体,对其进行表征,并进行小鼠黑色素瘤细胞摄取的初步研究。方法采用薄膜超声分散法制备Ce6和PFH共载的PEG化长循环脂质体(Ce6-PFH@PEG-Lip),运用单因素筛选法依次筛...目的制备共载光敏剂二氢卟吩(Ce6)和氧载体全氟己烷(PFH)的长循环脂质体,对其进行表征,并进行小鼠黑色素瘤细胞摄取的初步研究。方法采用薄膜超声分散法制备Ce6和PFH共载的PEG化长循环脂质体(Ce6-PFH@PEG-Lip),运用单因素筛选法依次筛选探头超声功率、有机溶剂种类、卵磷脂与胆固醇及DSPE-PEG的投料比、PFH和Ce6用量、大豆油和e80磷脂用量,以及制备工艺;并对所制得纳米粒的粒度分布、Zeta电位、形态、包封率和载药量进行表征。同时应用流式细胞术研究细胞摄取情况。结果筛选出最优处方,成功制备了均一稳定的Ce6-PFH@PEGLip,其粒径为133.9±3.1 nm,PDI为0.211,Zeta电位为-39.1±2.3 m V,对Ce6的包封率为98.09%±1.6%。DSPEPEG的加入使得制剂更加稳定,提高了细胞对纳米粒的摄取效率。结论Ce6和PFH具有良好的相容性,能应用简单的处方和薄膜超声分散法制成共载长循环脂质体,在缺氧性肿瘤光动力治疗的应用具有广阔前景。展开更多
以不同浓度的全氟己烷磺酸(PFHxS)分别灌胃暴露雄性Balb/c小鼠,连续暴露28 d后,收集小鼠的粪便,提取总DNA,并采用高通量测序技术对16S DNA V3-V4区进行测序。分析测序结果表明,28 d后最高剂量组小鼠体重显著性下降;随着PFHxS浓度的升高...以不同浓度的全氟己烷磺酸(PFHxS)分别灌胃暴露雄性Balb/c小鼠,连续暴露28 d后,收集小鼠的粪便,提取总DNA,并采用高通量测序技术对16S DNA V3-V4区进行测序。分析测序结果表明,28 d后最高剂量组小鼠体重显著性下降;随着PFHxS浓度的升高,小鼠肠道菌群OTU数增加;与对照组相比,暴露组肠道菌群多样性下降,Alpha多样性指数Chao值、Shannon指数差异均显著(p<0.05)。在门水平上,拟杆菌门占比显著升高;在属水平上,普氏菌属比例变化明显,类杆菌属、乳酸杆菌属比例随着暴露剂量的提高而增加。研究结果初步揭示了PFHxS暴露造成与小鼠糖脂代谢相关的肠道菌群发生明显变化,为研究肠道菌群改变与机体糖脂代谢功能紊乱的相关性奠定基础。展开更多
基金support of Chinese Natural Science Foundation(No.21277077,21325730)Ministry of Education(No.20130031130005)+1 种基金Ministry of Science and Technology(No.2012ZX07529-003)Ministry of Education Innovation Team(IRT 13024)
文摘The sorption and desorption behaviors of two perfluoroalkane sulfonates（PFSAs）, including perfluorohexane sulfonate（PFHx S） and perfluorooctane sulfonate（PFOS） on two humic acids（HAs） and humin（HM）, which were extracted from a peat soil, were investigated. The sorption kinetics and isotherms showed that the sorption of PFOS on the humic substances（HSs） was much higher than PFHx S. For the same PFSA compound, the sorption on HSs followed the order of HM 〉 HA2 〉 HA1. These suggest that hydrophobic interaction plays a key role in the sorption of PFSAs on HSs. The sorption capacities of PFSAs on HSs were significantly related to their aliphaticity, but negatively correlated to aromatic carbons,indicating the importance of aliphatic groups in the sorption of PFSAs. Compared to PFOS,PFHx S displayed distinct desorption hysteresis, probably due to irreversible pore deformation after sorption of PFHx S. The sorption of the two PFSAs on HSs decreased with an increase in p H in the solution. This is ascribed to the electrostatic interaction and hydrogen bonding at lower p H. Hydrophobic interaction might also be stronger at lower p H due to the aggregation of HSs.
基金supported by National Key Research and Development Project(No.2020YFE0204400)National Natural Science Foundation of China(No.52022090)Zhejiang Provincial Ten Thousand Talents Program(No.2018R52001).
文摘Hypoxia,an important characteristic of bacterial biofilms,can hinder the generation of reactive oxygen species(ROS)in photodynamic therapy(PDT),leading to reduced therapeutic efficacy of PDT.In order to address this issue,fluorinated liposome was fabricated as an oxygen-sufficient nanoplatform for enhanced photodynamic eradication of bacterial biofilms.The liposomes(denoted as Lip-Ce6-PFH@O_(2))were prepared by co-encapsulation of O_(2) carrier perfluorohexane(PFH)and photosensitizer chlorin e6(Ce_(6)).Lip-Ce6-PFH@O_(2) could achieve efficient biofilm penetration due to the positively charged surface.The hypoxic microenvironment of biofilms would then be relieved,leading to the generation of more ROS under laser irradiation.Therefore,the bactericidal capability of PDT could be significantly improved because of the co-delivered O_(2) carrier PFH.Lip-Ce6-PFH@O_(2) exhibited much better antibiofilm ability than that of Lip-Ce6 both in vitro and in vivo.Meanwhile,Lip-Ce6-PFH@O_(2) also effectively alleviated inflammation symptoms and accelerated wound healing in the mice model.In general,this study provides a new paradigm to enhance the therapeutic efficacy of PDT for efficient biofilm eradication.
文摘目的制备共载光敏剂二氢卟吩(Ce6)和氧载体全氟己烷(PFH)的长循环脂质体,对其进行表征,并进行小鼠黑色素瘤细胞摄取的初步研究。方法采用薄膜超声分散法制备Ce6和PFH共载的PEG化长循环脂质体(Ce6-PFH@PEG-Lip),运用单因素筛选法依次筛选探头超声功率、有机溶剂种类、卵磷脂与胆固醇及DSPE-PEG的投料比、PFH和Ce6用量、大豆油和e80磷脂用量,以及制备工艺;并对所制得纳米粒的粒度分布、Zeta电位、形态、包封率和载药量进行表征。同时应用流式细胞术研究细胞摄取情况。结果筛选出最优处方,成功制备了均一稳定的Ce6-PFH@PEGLip,其粒径为133.9±3.1 nm,PDI为0.211,Zeta电位为-39.1±2.3 m V,对Ce6的包封率为98.09%±1.6%。DSPEPEG的加入使得制剂更加稳定,提高了细胞对纳米粒的摄取效率。结论Ce6和PFH具有良好的相容性,能应用简单的处方和薄膜超声分散法制成共载长循环脂质体,在缺氧性肿瘤光动力治疗的应用具有广阔前景。
文摘以不同浓度的全氟己烷磺酸(PFHxS)分别灌胃暴露雄性Balb/c小鼠,连续暴露28 d后,收集小鼠的粪便,提取总DNA,并采用高通量测序技术对16S DNA V3-V4区进行测序。分析测序结果表明,28 d后最高剂量组小鼠体重显著性下降;随着PFHxS浓度的升高,小鼠肠道菌群OTU数增加;与对照组相比,暴露组肠道菌群多样性下降,Alpha多样性指数Chao值、Shannon指数差异均显著(p<0.05)。在门水平上,拟杆菌门占比显著升高;在属水平上,普氏菌属比例变化明显,类杆菌属、乳酸杆菌属比例随着暴露剂量的提高而增加。研究结果初步揭示了PFHxS暴露造成与小鼠糖脂代谢相关的肠道菌群发生明显变化,为研究肠道菌群改变与机体糖脂代谢功能紊乱的相关性奠定基础。
