Electroporation, PEG, PEG plus electroporation and Biolistics methods were tested ingene transformation of P. yezoensis. The exogenous gus was from plasmid of pBI121 and pCAMBI-A1301, both contain the CaMV35S promoter...Electroporation, PEG, PEG plus electroporation and Biolistics methods were tested ingene transformation of P. yezoensis. The exogenous gus was from plasmid of pBI121 and pCAMBI-A1301, both contain the CaMV35S promoter. The receptors included the prooplasts, tissues and free-liv-ing conchocelis filaments of P. yezoensis. Several factors, for example, the voltage, capacitance and bi-valent cations, etc., were studied. Results show that these four methods are all efficient for gene transfor-mation in P. yezoensis; and that PEG is the best one, with transformation efficiency of up to 4 ×10-5.GUS activity was detected 26 days after transfation by using PEG method.展开更多
文摘Electroporation, PEG, PEG plus electroporation and Biolistics methods were tested ingene transformation of P. yezoensis. The exogenous gus was from plasmid of pBI121 and pCAMBI-A1301, both contain the CaMV35S promoter. The receptors included the prooplasts, tissues and free-liv-ing conchocelis filaments of P. yezoensis. Several factors, for example, the voltage, capacitance and bi-valent cations, etc., were studied. Results show that these four methods are all efficient for gene transfor-mation in P. yezoensis; and that PEG is the best one, with transformation efficiency of up to 4 ×10-5.GUS activity was detected 26 days after transfation by using PEG method.
