AIM To elucidate the effect of various solutions for small bowel graft preservation in pigs under hypothermic storage. METHODS The swine segmental small bowel graft was autotransplanted after it was preserved with ...AIM To elucidate the effect of various solutions for small bowel graft preservation in pigs under hypothermic storage. METHODS The swine segmental small bowel graft was autotransplanted after it was preserved with lactated Ringer′s (LR), Euro Collins (EC), hyperosmolarity citrate adenine (HC A) and WMO 1 solutions for 10, 18 and 24 hours, respectively. The recipient survival rate, morphological structure, graft mucosal energy substances and Na + K + ATPase activity were studied, and graft absorption was estimated with D xylose absorption test. RESULTS The morphological study of the grafts preserved with LR or HC A solution for 10 hours or with EC and WMO 1 solution for 18 hours was normal 6 days after operation. Mucosal ATP, total adenine nucleotides (TAN) contents and Na + K + ATPase activity of the graft preserved with EC or WMO solution were higher than that of the graft preserved with LR or HC A solution. Serum level of D xylose was higher in EC and WMO 1 groups than in LR and HC A groups when the graft was preserved for 24 hours. CONCLUSIONS EC and WMO 1 solutions can preserve the swine small bowel up to 18 hours, which are superior to LR and HC A solutions.展开更多
文摘AIM To elucidate the effect of various solutions for small bowel graft preservation in pigs under hypothermic storage. METHODS The swine segmental small bowel graft was autotransplanted after it was preserved with lactated Ringer′s (LR), Euro Collins (EC), hyperosmolarity citrate adenine (HC A) and WMO 1 solutions for 10, 18 and 24 hours, respectively. The recipient survival rate, morphological structure, graft mucosal energy substances and Na + K + ATPase activity were studied, and graft absorption was estimated with D xylose absorption test. RESULTS The morphological study of the grafts preserved with LR or HC A solution for 10 hours or with EC and WMO 1 solution for 18 hours was normal 6 days after operation. Mucosal ATP, total adenine nucleotides (TAN) contents and Na + K + ATPase activity of the graft preserved with EC or WMO solution were higher than that of the graft preserved with LR or HC A solution. Serum level of D xylose was higher in EC and WMO 1 groups than in LR and HC A groups when the graft was preserved for 24 hours. CONCLUSIONS EC and WMO 1 solutions can preserve the swine small bowel up to 18 hours, which are superior to LR and HC A solutions.
