AIM: NGX6, NAG-7 and BRD7 genes are tumor related genes, which have been newly cloned by positional candidate cloning strategy. This study was designed to investigate the expression levels of NGX6, NAG-7 and BRD7 gene...AIM: NGX6, NAG-7 and BRD7 genes are tumor related genes, which have been newly cloned by positional candidate cloning strategy. This study was designed to investigate the expression levels of NGX6, NAG-7 and BRD7 genes in human gastric and colorectal cancer tissues, and their corresponding normal tissues, and to investigate whether these genes play a role in the pathogenesis of gastric and colorectal cancers.METHODS: Reverse transcription-polymerase chain reaction (RT-PCR), dot hybridization and Northern blot analysis were used to compare the expression levels of NGX6, NAG-7 and BRD7 genes in 34 gastric cancer tissues and 34 colorectal cancer tissues with their corresponding normal tissues of the same patients, respectively.RESULTS: Among the 34 colorectal cancer specimens and the 34 gastric cancer specimens, the expression of NGX6 in 25 colorectal cancer tissues was absent or very weak (73.5 %) by RT-PCR analysis. The down-regulation rate of NGX6 in colorectal cancer tissues was significantly higher than that in corresponding normal tissues (26.5 %,9/34)(P＜0.005). Moreover, the down-regulation of NGX6 was significantly correlated with lymph node and/or distance metastases. Patients with lymph node and/or distance metastasis had much higher down-regulation rate of NGX6 than patients without metastases (93.8 % vs 55.6 %, P＜0.05).However no correlation was found between the expression of NGX6 and pathologic type of colorectal cancer in this study, and also the expression of NGX6 did not display any difference between gastric cancer and corresponding normal tissues (58.8 % vs 70.6 %, P＞0.25). Dot hybridization and Northern blot analysis confirmed the results of RT-PCR.Furthermore, NAG-7 and BRD7 mRNA was not up- or downregulated in gastric and colorectal cancers compared with their corresponding normal tissues in our study.CONCLUSION: The down-regulation of NGX6 may be closely associated with tumorigenesis and metastasis of colorectal carcinoma. However, it may not contribute to the development and progression展开更多
目的:探讨NGX6在乳腺癌中的表达及与乳腺影像报告数据系统(breast imaging reporting and data system, BI-RADS)超声特征及淋巴结转移的关系。方法:回顾性收集162例乳腺癌的超声声像图特征及临床资料,参照BI-RADS标准予以分类。免疫组...目的:探讨NGX6在乳腺癌中的表达及与乳腺影像报告数据系统(breast imaging reporting and data system, BI-RADS)超声特征及淋巴结转移的关系。方法:回顾性收集162例乳腺癌的超声声像图特征及临床资料,参照BI-RADS标准予以分类。免疫组织化学法检测乳腺癌组织中NGX6的表达,分析乳腺癌组织NGX6表达与BI-RADS超声特征及淋巴结转移的关系。结果:在162例乳腺癌中,92例(56.8%)NGX6阳性表达,70例(43.2%)NGX6阴性表达。NGX6阴性表达组的乳腺癌肿块血流丰富、乳腺癌BI-RADS高类别发生率及淋巴结转移发生率均高于NGX6阳性表达组。NGX6表达与肿瘤血流程度及BI-RADS超声分类呈负相关(分别P=0.043,P=0.020,P=0.005)。结论:NGX6作为一个抑癌基因,与乳腺癌BI-RADS超声分类及超声生物学特征相关,NGX6表达水平在一定程度上可反映乳腺癌生物学行为。展开更多
基金the Natural Science Foundation of Hunan Province No.01JJY2102the National"863 Program"of China,No.102-10-01-05
文摘AIM: NGX6, NAG-7 and BRD7 genes are tumor related genes, which have been newly cloned by positional candidate cloning strategy. This study was designed to investigate the expression levels of NGX6, NAG-7 and BRD7 genes in human gastric and colorectal cancer tissues, and their corresponding normal tissues, and to investigate whether these genes play a role in the pathogenesis of gastric and colorectal cancers.METHODS: Reverse transcription-polymerase chain reaction (RT-PCR), dot hybridization and Northern blot analysis were used to compare the expression levels of NGX6, NAG-7 and BRD7 genes in 34 gastric cancer tissues and 34 colorectal cancer tissues with their corresponding normal tissues of the same patients, respectively.RESULTS: Among the 34 colorectal cancer specimens and the 34 gastric cancer specimens, the expression of NGX6 in 25 colorectal cancer tissues was absent or very weak (73.5 %) by RT-PCR analysis. The down-regulation rate of NGX6 in colorectal cancer tissues was significantly higher than that in corresponding normal tissues (26.5 %,9/34)(P＜0.005). Moreover, the down-regulation of NGX6 was significantly correlated with lymph node and/or distance metastases. Patients with lymph node and/or distance metastasis had much higher down-regulation rate of NGX6 than patients without metastases (93.8 % vs 55.6 %, P＜0.05).However no correlation was found between the expression of NGX6 and pathologic type of colorectal cancer in this study, and also the expression of NGX6 did not display any difference between gastric cancer and corresponding normal tissues (58.8 % vs 70.6 %, P＞0.25). Dot hybridization and Northern blot analysis confirmed the results of RT-PCR.Furthermore, NAG-7 and BRD7 mRNA was not up- or downregulated in gastric and colorectal cancers compared with their corresponding normal tissues in our study.CONCLUSION: The down-regulation of NGX6 may be closely associated with tumorigenesis and metastasis of colorectal carcinoma. However, it may not contribute to the development and progression
文摘目的:探讨NGX6在乳腺癌中的表达及与乳腺影像报告数据系统(breast imaging reporting and data system, BI-RADS)超声特征及淋巴结转移的关系。方法:回顾性收集162例乳腺癌的超声声像图特征及临床资料,参照BI-RADS标准予以分类。免疫组织化学法检测乳腺癌组织中NGX6的表达,分析乳腺癌组织NGX6表达与BI-RADS超声特征及淋巴结转移的关系。结果:在162例乳腺癌中,92例(56.8%)NGX6阳性表达,70例(43.2%)NGX6阴性表达。NGX6阴性表达组的乳腺癌肿块血流丰富、乳腺癌BI-RADS高类别发生率及淋巴结转移发生率均高于NGX6阳性表达组。NGX6表达与肿瘤血流程度及BI-RADS超声分类呈负相关(分别P=0.043,P=0.020,P=0.005)。结论:NGX6作为一个抑癌基因,与乳腺癌BI-RADS超声分类及超声生物学特征相关,NGX6表达水平在一定程度上可反映乳腺癌生物学行为。
