The heat shock response is a general property of all living organisms. Both heat shock response and heat shock proteins (HSPs) exist in cells and the role of heat shock proteins acts as molecular chaperones. Heat shoc...The heat shock response is a general property of all living organisms. Both heat shock response and heat shock proteins (HSPs) exist in cells and the role of heat shock proteins acts as molecular chaperones. Heat shock response was first recognized in insects. The purpose of this paper is to introduce a brief history of the research on heat shock proteins and to update progress with emphasis on two aspects, namely: the structure of heat shock genes and the transcriptional control of heat shock genes in insects ; the expression models, the properties of heat shock proteins, the intracellular location of HSPs in cells and the role of heat shock proteins as molecular chaprones. In general, the study of heat shock response and heat shock proteins goes through five stages. Heat shock genes that are classified into three main distinct groups characterized by sequence homology are considerably conservative. Heat shock genes in insects are controlled by the heat shock transcription factors. Most heat shock proteins are synthesized at normal conditions, but their syntheses significantly increase at heat shock or other stress conditions. The syntheses also change with the development stages and sorts of tissues. The intracellular location of HSPs varies according to HSPs themselves and the condition that the cells are in. Heat shock proteins act as molecular chaperones to regulate protein folding, protein translocation, assembly and disassembly. The synthesis of the heat shock proteins is correlated with the acquired thermotolerance and maybe used in gene engineering and has beneficial therapeutic effects on tumor treatments.展开更多
We report the thermotolerance of new bivoltine silkworm, Bombyx mori strains NB4D2, KSO1, NP2, CSR2 and CSR4 and differential expression of heat shock proteins at different instars. Different instars of silkworm larva...We report the thermotolerance of new bivoltine silkworm, Bombyx mori strains NB4D2, KSO1, NP2, CSR2 and CSR4 and differential expression of heat shock proteins at different instars. Different instars of silkworm larva were subjected to heat shock at 35℃, 40℃ and 45℃ for 2 hours followed by 2 hours recovery. Heat shock proteins were analyzed by SDS-PAGE. The impact of heat shock on commercial traits of cocoons was analyzed by following different strategies in terms of acquired thermotolerance over control. Comparatively NP2 exhibited better survivability than other strains. Resistance to heat shock was increased as larval development proceeds in the order of first instar 〉 second instar 〉 third instar 〉 fourth instar 〉 fifth instar in all silkworm strains. Expression of heat shock proteins varies in different instars. 90 kDa in the first, second and third instars, 84 kDa in the fourth instar and 84, 62, 60, 47 and 33 kDa heat shock proteins in fifth instar was observed in response to heat shock. Relative influence of heat shock on commercial traits that correspond to different stages was significant in all strains. In NB4D2, cocoon and shell weight significantly increased to 17.52% and 19.44% over control respectively. Heat shock proteins as molecular markers for evaluation and evolution of thermotolerant silkworm strains for tropics was discussed.展开更多
文摘The heat shock response is a general property of all living organisms. Both heat shock response and heat shock proteins (HSPs) exist in cells and the role of heat shock proteins acts as molecular chaperones. Heat shock response was first recognized in insects. The purpose of this paper is to introduce a brief history of the research on heat shock proteins and to update progress with emphasis on two aspects, namely: the structure of heat shock genes and the transcriptional control of heat shock genes in insects ; the expression models, the properties of heat shock proteins, the intracellular location of HSPs in cells and the role of heat shock proteins as molecular chaprones. In general, the study of heat shock response and heat shock proteins goes through five stages. Heat shock genes that are classified into three main distinct groups characterized by sequence homology are considerably conservative. Heat shock genes in insects are controlled by the heat shock transcription factors. Most heat shock proteins are synthesized at normal conditions, but their syntheses significantly increase at heat shock or other stress conditions. The syntheses also change with the development stages and sorts of tissues. The intracellular location of HSPs varies according to HSPs themselves and the condition that the cells are in. Heat shock proteins act as molecular chaperones to regulate protein folding, protein translocation, assembly and disassembly. The synthesis of the heat shock proteins is correlated with the acquired thermotolerance and maybe used in gene engineering and has beneficial therapeutic effects on tumor treatments.
文摘We report the thermotolerance of new bivoltine silkworm, Bombyx mori strains NB4D2, KSO1, NP2, CSR2 and CSR4 and differential expression of heat shock proteins at different instars. Different instars of silkworm larva were subjected to heat shock at 35℃, 40℃ and 45℃ for 2 hours followed by 2 hours recovery. Heat shock proteins were analyzed by SDS-PAGE. The impact of heat shock on commercial traits of cocoons was analyzed by following different strategies in terms of acquired thermotolerance over control. Comparatively NP2 exhibited better survivability than other strains. Resistance to heat shock was increased as larval development proceeds in the order of first instar 〉 second instar 〉 third instar 〉 fourth instar 〉 fifth instar in all silkworm strains. Expression of heat shock proteins varies in different instars. 90 kDa in the first, second and third instars, 84 kDa in the fourth instar and 84, 62, 60, 47 and 33 kDa heat shock proteins in fifth instar was observed in response to heat shock. Relative influence of heat shock on commercial traits that correspond to different stages was significant in all strains. In NB4D2, cocoon and shell weight significantly increased to 17.52% and 19.44% over control respectively. Heat shock proteins as molecular markers for evaluation and evolution of thermotolerant silkworm strains for tropics was discussed.
