As a member of the Frizzled family, Frizzled3 (FZD3) is a receptor of the canonical Wnt signaling pathway and plays a vital role in mammalian hair follicle developmental processes. However, its effects on wool traits ...As a member of the Frizzled family, Frizzled3 (FZD3) is a receptor of the canonical Wnt signaling pathway and plays a vital role in mammalian hair follicle developmental processes. However, its effects on wool traits are not clear. The objectives of this study were to identify the single nucleotide polymorphisms (SNPs) and the expression patterns of FZD3 gene, and then to determine whether it affected wool traits of Chinese Merino sheep (Xinjiang Type) or not. PCR-single stranded conformational polymorphism (PCR-SSCP) and sequencing were used to identify mutation loci, and general linear model (GLM) with SAS 9.1 was used for the association analysis between wool traits and SNPs. Quantitative real-time PCR (qRT-PCR) was used to investigate FZD3 gene expression levels. The results showed that six exons of FZD3 gene were amplified and two mutation loci were identified in exon 1 (NC_019459.2: g.101771685 T>C (SNP1)) and exon 3 (NC_019459.2: g.101810848, A>C (SNP2)), respectively. Association analysis showed that SNP1 was significantly associated with mean fiber diameter (MFD)(P=0.04) and live weight (LW)(P=0.0004), SNP2 was significantly associated with greasy fleece weight (GFW)(P=0.04). The expression level of FZD3 gene in skin tissues of the superfine wool (SF) group was significantly lower (P<0.05) than that of the fine wool (F) group. Moreover, it had a higher expression level (P<0.01) in skin tissues than in other tissues of Chinese Merino ewes. While, its expression level had a fluctuant expression in skin tissues at different developmental stages of embryos and born lambs, with the highest expression levels (P<0.01) at the 65th day of embryos. Our study revealed the genetic relationship between FZD3 variants and wool traits and two identified SNPs might serve as potential and valuable genetic markers for sheep breeding and lay a molecular genetic foundation for sheep marker-assisted selection (MAS).展开更多
[ Objective] The study aimed to analyze the expression and polymorphism of KAP6.1 gene in various tissues of sheep, as well as the correlation between KAP6.1 gene and wool traits, so as to provide scientific reference...[ Objective] The study aimed to analyze the expression and polymorphism of KAP6.1 gene in various tissues of sheep, as well as the correlation between KAP6.1 gene and wool traits, so as to provide scientific references for the further study on the functions of KAP6.1 gene and molecular breeding of fine wool sheep. [Method] By using real-time fluorescence quantitative PCR, the expression map of KAP6.1 gene in various tissues was analyzed, and then PCR-SSCP technology, cloning and sequencing were adopted to analyze the polymorphism of KAP6.1 gene in 693 Chinese merino sheep （Xinjiang Junken type）, finally the correlation between KAPS. 1 gene and wool traits was discussed. [ Result] A high -level expression of KAP6.1 gene appeared in sheep skin, very significantly higher than that in muscle, small intestines, ovaries, hearts, lungs, livers, spleens, kidneys and rumen （ P 〈0.01 ）. In addition, there was C159T base substitution in KAF6.1 gene sequence, and analysis of the least squares model showed that the mean wool fleece weight of BB genotype at C159T site was significantly higher than that of AA and AB genotype （ P 〈0.05）, while there was no significant difference in average wool fiber diameter, curvature, length, clean fleece rate and density among hA, BB and AB genotype of KAP6.1 gene at C159T site （P〉0.05）. [Conclusion] KAP6.1 gene could be as the candidate gene of wool yield of sheep, and BB genotype could be used as the important molecular marker of fine wool sheep for high wool yield.展开更多
[Objective] The paper was to study the adaptability of Australian Merino sheep in the alpine pastoral areas. [Method]Through introduction of Australian Merino sheep for hybridizing with Gansu alpine fine wool sheep,th...[Objective] The paper was to study the adaptability of Australian Merino sheep in the alpine pastoral areas. [Method]Through introduction of Australian Merino sheep for hybridizing with Gansu alpine fine wool sheep,the improvement effect of offspring was observed. [Result]The introduced Australian Merino sheep had good adaptability in the alpine pastoral areas with good hair quality,strong cold resistance and disease resistance,and they could grow and reproduce healthily in the alpine pastoral areas. The application effect of the ram was outstanding,showing large breeding amount,high conception rate and survival rate.[Conclusion]The improvement effect of offspring of Australian Merino sheep and Gansu alpine fine wool sheep was significant,and the growth performance was improved greatly.展开更多
“Indigenous vaccine” developed from native “Indian Bison Type” strain (“S5”) of Mycobacterium avium subspecies paratuberculosis (MAP) of “goat origin” was first time evaluated in a sheep flock of Bharat Merino...“Indigenous vaccine” developed from native “Indian Bison Type” strain (“S5”) of Mycobacterium avium subspecies paratuberculosis (MAP) of “goat origin” was first time evaluated in a sheep flock of Bharat Merino breed located in Mannavanur town of Tamil Nadu in South India. Therapeutic efficacy of the vaccine was evaluated for 3 years between 2008 and 2010, on the basis of improvements in productivity (body weights, reproductive efficiency and survivability), physical condition, clinical symptoms (weakness, diarrhea, wool quality), immune response (sero-conversion) and infection load in feces (shedding). After immunization of the flock in 2008, the successive progenies of 112 and 53 lambs born in 2009 and 2010, respectively were vaccinated. Whereas, 40 lambs born to control animals were kept as unvaccinated controls. Though gain in body weights in vaccinated versus controls were not significant in 2008, growth rates were distinctly superior in Ist and IInd generations of vaccinated lambs. Reproductive performance (tupping percent) and survivability of lambs and adult sheep improved significantly. There was overall reduction in yearly morbidity (diarrhea) and mortality rates of the flock in post vaccination years. Shedding of MAP in feces was reduced in vaccinated sheep by 6.2%, 14.3% and 27.3% in 2008, 2009 and 2010 respectively, whereas shedding increased in the control sheep. Seromonitoring of the animals by “indigenous ELISA kit” showed enhanced “flock immunity” in successive generations. “Indigenous vaccine” reduced clinical disease and shedding and improved immunity and productivity of Bharat Merino flock, endemic for Johne’s disease.展开更多
基金supported by the National Natural Science Foundation of China (31360543 and 31760655)the earmarked fund for the China Agriculture Research System (CARS-39)+1 种基金the Open Project of Key Laboratory of Genetics Breeding and Reproduction of Xinjiang Cashmere and Wool Sheep, China (2016D03017)the Postdoctoral Science Foundation, China (2017M623287)
文摘As a member of the Frizzled family, Frizzled3 (FZD3) is a receptor of the canonical Wnt signaling pathway and plays a vital role in mammalian hair follicle developmental processes. However, its effects on wool traits are not clear. The objectives of this study were to identify the single nucleotide polymorphisms (SNPs) and the expression patterns of FZD3 gene, and then to determine whether it affected wool traits of Chinese Merino sheep (Xinjiang Type) or not. PCR-single stranded conformational polymorphism (PCR-SSCP) and sequencing were used to identify mutation loci, and general linear model (GLM) with SAS 9.1 was used for the association analysis between wool traits and SNPs. Quantitative real-time PCR (qRT-PCR) was used to investigate FZD3 gene expression levels. The results showed that six exons of FZD3 gene were amplified and two mutation loci were identified in exon 1 (NC_019459.2: g.101771685 T>C (SNP1)) and exon 3 (NC_019459.2: g.101810848, A>C (SNP2)), respectively. Association analysis showed that SNP1 was significantly associated with mean fiber diameter (MFD)(P=0.04) and live weight (LW)(P=0.0004), SNP2 was significantly associated with greasy fleece weight (GFW)(P=0.04). The expression level of FZD3 gene in skin tissues of the superfine wool (SF) group was significantly lower (P<0.05) than that of the fine wool (F) group. Moreover, it had a higher expression level (P<0.01) in skin tissues than in other tissues of Chinese Merino ewes. While, its expression level had a fluctuant expression in skin tissues at different developmental stages of embryos and born lambs, with the highest expression levels (P<0.01) at the 65th day of embryos. Our study revealed the genetic relationship between FZD3 variants and wool traits and two identified SNPs might serve as potential and valuable genetic markers for sheep breeding and lay a molecular genetic foundation for sheep marker-assisted selection (MAS).
基金supported by Genetically Modified Organisms Breeding Major Projects ( 2009ZX08009-160B) Science and Technology Guidance Plan of Xinjiang Academy of Agricultural and Reclamation Sciences (YYD2010-8)+1 种基金 Science and Technology Key Project of Bintuan Agriculture (2009GG17,2011BA006) Doctoral Foundation of Bintuan (2011BB015)
文摘[ Objective] The study aimed to analyze the expression and polymorphism of KAP6.1 gene in various tissues of sheep, as well as the correlation between KAP6.1 gene and wool traits, so as to provide scientific references for the further study on the functions of KAP6.1 gene and molecular breeding of fine wool sheep. [Method] By using real-time fluorescence quantitative PCR, the expression map of KAP6.1 gene in various tissues was analyzed, and then PCR-SSCP technology, cloning and sequencing were adopted to analyze the polymorphism of KAP6.1 gene in 693 Chinese merino sheep （Xinjiang Junken type）, finally the correlation between KAPS. 1 gene and wool traits was discussed. [ Result] A high -level expression of KAP6.1 gene appeared in sheep skin, very significantly higher than that in muscle, small intestines, ovaries, hearts, lungs, livers, spleens, kidneys and rumen （ P 〈0.01 ）. In addition, there was C159T base substitution in KAF6.1 gene sequence, and analysis of the least squares model showed that the mean wool fleece weight of BB genotype at C159T site was significantly higher than that of AA and AB genotype （ P 〈0.05）, while there was no significant difference in average wool fiber diameter, curvature, length, clean fleece rate and density among hA, BB and AB genotype of KAP6.1 gene at C159T site （P〉0.05）. [Conclusion] KAP6.1 gene could be as the candidate gene of wool yield of sheep, and BB genotype could be used as the important molecular marker of fine wool sheep for high wool yield.
基金Supported by Special Fund for Construction of National Wool-purpose Sheep Industry Research System(CARS-40-33)
文摘[Objective] The paper was to study the adaptability of Australian Merino sheep in the alpine pastoral areas. [Method]Through introduction of Australian Merino sheep for hybridizing with Gansu alpine fine wool sheep,the improvement effect of offspring was observed. [Result]The introduced Australian Merino sheep had good adaptability in the alpine pastoral areas with good hair quality,strong cold resistance and disease resistance,and they could grow and reproduce healthily in the alpine pastoral areas. The application effect of the ram was outstanding,showing large breeding amount,high conception rate and survival rate.[Conclusion]The improvement effect of offspring of Australian Merino sheep and Gansu alpine fine wool sheep was significant,and the growth performance was improved greatly.
文摘“Indigenous vaccine” developed from native “Indian Bison Type” strain (“S5”) of Mycobacterium avium subspecies paratuberculosis (MAP) of “goat origin” was first time evaluated in a sheep flock of Bharat Merino breed located in Mannavanur town of Tamil Nadu in South India. Therapeutic efficacy of the vaccine was evaluated for 3 years between 2008 and 2010, on the basis of improvements in productivity (body weights, reproductive efficiency and survivability), physical condition, clinical symptoms (weakness, diarrhea, wool quality), immune response (sero-conversion) and infection load in feces (shedding). After immunization of the flock in 2008, the successive progenies of 112 and 53 lambs born in 2009 and 2010, respectively were vaccinated. Whereas, 40 lambs born to control animals were kept as unvaccinated controls. Though gain in body weights in vaccinated versus controls were not significant in 2008, growth rates were distinctly superior in Ist and IInd generations of vaccinated lambs. Reproductive performance (tupping percent) and survivability of lambs and adult sheep improved significantly. There was overall reduction in yearly morbidity (diarrhea) and mortality rates of the flock in post vaccination years. Shedding of MAP in feces was reduced in vaccinated sheep by 6.2%, 14.3% and 27.3% in 2008, 2009 and 2010 respectively, whereas shedding increased in the control sheep. Seromonitoring of the animals by “indigenous ELISA kit” showed enhanced “flock immunity” in successive generations. “Indigenous vaccine” reduced clinical disease and shedding and improved immunity and productivity of Bharat Merino flock, endemic for Johne’s disease.
