目的:分析连花清瘟制剂的相关文献,阐明该领域的研究现状和前沿热点,为临床合理应用和深入研究提供参考。方法:检索中国知网、维普网、万方数据库、PubMed和Web of Science核心合集等6个数据库近10年收录的连花清瘟制剂相关文献,借助Not...目的:分析连花清瘟制剂的相关文献,阐明该领域的研究现状和前沿热点,为临床合理应用和深入研究提供参考。方法:检索中国知网、维普网、万方数据库、PubMed和Web of Science核心合集等6个数据库近10年收录的连花清瘟制剂相关文献,借助NoteExpress和CiteSpace对文献进行管理和分析。结果:最终分别纳入中英文文献344篇和76篇,近年来发文增多。文献发表于162种中文和48种英文期刊。石家庄以岭药业股份有限公司和广州医科大学分别是发文量最多的中英文机构。发文量最多的作者是刘敏彦。频数较高的关键词有临床疗效、连花清瘟、炎性因子、中医药(traditional Chinese medicine)和新型冠状病毒肺炎(COVID-19)等。共生成临床疗效、中药、连花清瘟、COVID-19和甲型流感病毒(influenza a virus)等19个聚类,带状疱疹、肺炎、炎性因子、流感(influenza)和肠道微生物群(gut microbiota)等47个突现关键词。结论:该领域合作交流不足,研究热点为连花清瘟治疗新冠肺炎等疾病的临床疗效、药物抗病毒等药理作用和机制、微观机制研究侧重于相关通路与靶点蛋白等和中西药联合用药等方面。展开更多
Objective To investigate the antagonistic effects of different doses of Lianhua Qingwen on pulmonary injury induced by fine particulates PM2.5 in rats. Methods Fine particulates suspended in the environment were colle...Objective To investigate the antagonistic effects of different doses of Lianhua Qingwen on pulmonary injury induced by fine particulates PM2.5 in rats. Methods Fine particulates suspended in the environment were collected. Forty-eight healthy adult wistar rats were randomly divided into 6 groups with 8 rats in each group. Four groups of rats were exposed to PM2.5 by intratracheally dripping suspensions of fine particulates PM2.5(7.5 mg/kg) as dust-exposed model rats. Among them 24 rats in three groups received Lianhua Qingwen treatment(crude drug) at a dose of 2 g/kg, 4 g/kg, 8 g/kg per day for 3 days before dust exposure and were defined as low-dose, middle-dose and high-dose Lianhua Qingwen treatment groups respectively. The other dust-exposed model rats without treatment were assigned as PM2.5 control group. The un-exposed rats were set as saline control group(1.5 ml/kg saline) and blank control group. All rats were killed after 24 hours of the exposure. Lung tissue, serum and bronchoalveolar lavage fluid(BALF) were collected. The levels of malonaldehyde(MDA), lactate dehydrogenase(LDH), and glutathione peroxidase(GSH-PX) in blood serum and BALF, and superoxide dismutase(SOD) in blood surum were measured using fluorescent quantitation PCR; Expression of NF-E2-related factor 2(NRF-2), heme oxygenase 1(HO-1) and quinone oxidoreductase 1(NQO1) in lung tissues were measured using Western blot. Pathological changes of lung tissues in each group were also examined. Results Pathology revealed thickened alveolar septum, congestion of capillary, interstitial edema and infiltration of lymphocyte and neutrophil surrounding bronchiole in the PM2.5 control group, which weresignificantly relieved in the Lianhua Qingwen treatment groups. Compared to the blank and saline control groups, the PM2.5 control group had significantly higher levels of LDH and MDA(p<0.01) and lower level of GSH-PS(p<0.01) in BALF, significantly higher levels of LDH and MDA(p<0.05) and lower level of GSH-PS(p<0.05) in rat serum. The levels of MDA in b展开更多
文摘目的:分析连花清瘟制剂的相关文献,阐明该领域的研究现状和前沿热点,为临床合理应用和深入研究提供参考。方法:检索中国知网、维普网、万方数据库、PubMed和Web of Science核心合集等6个数据库近10年收录的连花清瘟制剂相关文献,借助NoteExpress和CiteSpace对文献进行管理和分析。结果:最终分别纳入中英文文献344篇和76篇,近年来发文增多。文献发表于162种中文和48种英文期刊。石家庄以岭药业股份有限公司和广州医科大学分别是发文量最多的中英文机构。发文量最多的作者是刘敏彦。频数较高的关键词有临床疗效、连花清瘟、炎性因子、中医药(traditional Chinese medicine)和新型冠状病毒肺炎(COVID-19)等。共生成临床疗效、中药、连花清瘟、COVID-19和甲型流感病毒(influenza a virus)等19个聚类,带状疱疹、肺炎、炎性因子、流感(influenza)和肠道微生物群(gut microbiota)等47个突现关键词。结论:该领域合作交流不足,研究热点为连花清瘟治疗新冠肺炎等疾病的临床疗效、药物抗病毒等药理作用和机制、微观机制研究侧重于相关通路与靶点蛋白等和中西药联合用药等方面。
文摘Objective To investigate the antagonistic effects of different doses of Lianhua Qingwen on pulmonary injury induced by fine particulates PM2.5 in rats. Methods Fine particulates suspended in the environment were collected. Forty-eight healthy adult wistar rats were randomly divided into 6 groups with 8 rats in each group. Four groups of rats were exposed to PM2.5 by intratracheally dripping suspensions of fine particulates PM2.5(7.5 mg/kg) as dust-exposed model rats. Among them 24 rats in three groups received Lianhua Qingwen treatment(crude drug) at a dose of 2 g/kg, 4 g/kg, 8 g/kg per day for 3 days before dust exposure and were defined as low-dose, middle-dose and high-dose Lianhua Qingwen treatment groups respectively. The other dust-exposed model rats without treatment were assigned as PM2.5 control group. The un-exposed rats were set as saline control group(1.5 ml/kg saline) and blank control group. All rats were killed after 24 hours of the exposure. Lung tissue, serum and bronchoalveolar lavage fluid(BALF) were collected. The levels of malonaldehyde(MDA), lactate dehydrogenase(LDH), and glutathione peroxidase(GSH-PX) in blood serum and BALF, and superoxide dismutase(SOD) in blood surum were measured using fluorescent quantitation PCR; Expression of NF-E2-related factor 2(NRF-2), heme oxygenase 1(HO-1) and quinone oxidoreductase 1(NQO1) in lung tissues were measured using Western blot. Pathological changes of lung tissues in each group were also examined. Results Pathology revealed thickened alveolar septum, congestion of capillary, interstitial edema and infiltration of lymphocyte and neutrophil surrounding bronchiole in the PM2.5 control group, which weresignificantly relieved in the Lianhua Qingwen treatment groups. Compared to the blank and saline control groups, the PM2.5 control group had significantly higher levels of LDH and MDA(p<0.01) and lower level of GSH-PS(p<0.01) in BALF, significantly higher levels of LDH and MDA(p<0.05) and lower level of GSH-PS(p<0.05) in rat serum. The levels of MDA in b
