根据GenBank中已登录的猪旋毛虫49 ku ES抗原基因序列设计了1对引物,用SDS裂解液配合蛋白酶K方法从猪旋毛虫不同发育时期(成囊前期幼虫、肌幼虫、成虫)虫体中提取总RNA,用RT-PCR方法扩增49 ku ES抗原基因,将目的基因定向克隆入pMD18-T载...根据GenBank中已登录的猪旋毛虫49 ku ES抗原基因序列设计了1对引物,用SDS裂解液配合蛋白酶K方法从猪旋毛虫不同发育时期(成囊前期幼虫、肌幼虫、成虫)虫体中提取总RNA,用RT-PCR方法扩增49 ku ES抗原基因,将目的基因定向克隆入pMD18-T载体,转化大肠埃希氏菌TG1。用PCR、限制性内切酶EcoRⅠ和BamHⅠ进行单、双酶切鉴定,阳性质粒的测序结果表明,成功克隆了猪旋毛虫不同发育时期虫体49 ku ES抗原基因。序列分析结果显示:49 kuES抗原基因大小为948 bp,基因的保守性很强,序列同源性比较高,不同发育时期之间的差异很小。展开更多
Scutellariae Radix(SR), the root of Scutellaria baicalensis Georgi, is used as an antipyretic drug and has been demonstrated to have anti-inflammatory activity. SR is divided into two specifications, "Ku Qin"...Scutellariae Radix(SR), the root of Scutellaria baicalensis Georgi, is used as an antipyretic drug and has been demonstrated to have anti-inflammatory activity. SR is divided into two specifications, "Ku Qin"(KQ) and "Zi Qin"(ZQ), for use against different symptoms(upper energizer heat or lower portion of the triple energizer), according to the theory of traditional Chinese medicine(TCM). However, differences in the efficacies of these two specifications have not been determined. In the present study, we aimed to characterize the differences in the anti-inflammatory activities between KQ and ZQ and to explore how their differences are manifested in lipopolysaccharide(LPS)-induced macrophages. Our results showed that, in RAW264.7 cells(a mouse macrophage cell line derived from ascites), KQ and ZQ displayed anti-inflammatory effects by inhibiting the release of nitric oxide(NO), inducible NOS(iNOS), and nuclear factor-κB(NF-κB) in a dose-dependent manner without distinction. In NR8383 cells(a rat alveolar macrophage cell line), KQ and ZQ displayed similar effects on NO, iNOS, and NF-κB as seen in RAW264.7 cells, but KQ showed a higher inhibition rate for NO and i NOS than that shown by ZQ at the same concentration. These results indicated that there were differences in efficacy between KQ and ZQ in treating lung inflammation. Our findings provided an experimental evidence supporting the different uses of KQ and ZQ in clinic, as noted in ancient herbal records.展开更多
The 33 ku protein, prepared from NaCI-treated PSII particles, has shown an single band by SDS-PAGE. After being dialyzed against the low-osmotic medium at 4癈, it has been found that the 33 ku protein degraded into se...The 33 ku protein, prepared from NaCI-treated PSII particles, has shown an single band by SDS-PAGE. After being dialyzed against the low-osmotic medium at 4癈, it has been found that the 33 ku protein degraded into several small fragments. This result suggests that the preparations of 33 ku protein probably contain some latent proteinases. it has also been found, by the 2-D electrophoresis and IEF, that the preparations of 33 ku protein not dialyzed against the low-osmotic medium contain several polypeptides with nearly the same molecular weight but not the same isoelectric point as the 33 ku protein.展开更多
Nonhomologous DNA end joining （NHEJ） is the primary pathway for repair of double-strand DNA breaks in human cells and in multicellular eukaryotes. The causes of double-strand breaks often fragment the DNA at the sit...Nonhomologous DNA end joining （NHEJ） is the primary pathway for repair of double-strand DNA breaks in human cells and in multicellular eukaryotes. The causes of double-strand breaks often fragment the DNA at the site of damage, resulting in the loss of information there. NHEJ does not restore the lost information and may resect additional nucleotides during the repair process. The ability to repair a wide range of overhang and damage configurations reflects the flexibility of the nuclease, polymerases, and ligase of NHEJ. The flexibility of the individual components also explains the large number of ways in which NHEJ can repair any given pair of DNA ends. The loss of information locally at sites of NHEJ repair may contribute to cancer and aging, but the action by NHEJ ensures that entire segments of chromosomes are not lost.展开更多
As one of the carriers of Confucian culture,and from ancient times to the present,Lunyu or The Analects or The Analects of Confucius,has been well received by readers at home and abroad,and there are more and more Eng...As one of the carriers of Confucian culture,and from ancient times to the present,Lunyu or The Analects or The Analects of Confucius,has been well received by readers at home and abroad,and there are more and more English translations of Lunyu.This article will select some of the vocabulary translations with ancient Chinese characteristics in Lunyu as the object,employ the method of a comparative analysis to explore the cultural connotation behind the text and the dissemination of the translation,and discuss the translator’s translation strategies.Judging from the research on The Analects by scholars from home and abroad and the opening and development of overseas Confucius Institutes,Lunyu has exerted an enlightenment effect in different times.展开更多
文摘根据GenBank中已登录的猪旋毛虫49 ku ES抗原基因序列设计了1对引物,用SDS裂解液配合蛋白酶K方法从猪旋毛虫不同发育时期(成囊前期幼虫、肌幼虫、成虫)虫体中提取总RNA,用RT-PCR方法扩增49 ku ES抗原基因,将目的基因定向克隆入pMD18-T载体,转化大肠埃希氏菌TG1。用PCR、限制性内切酶EcoRⅠ和BamHⅠ进行单、双酶切鉴定,阳性质粒的测序结果表明,成功克隆了猪旋毛虫不同发育时期虫体49 ku ES抗原基因。序列分析结果显示:49 kuES抗原基因大小为948 bp,基因的保守性很强,序列同源性比较高,不同发育时期之间的差异很小。
基金supported by the National Natural Science Foundation of China(No.81373921)
文摘Scutellariae Radix(SR), the root of Scutellaria baicalensis Georgi, is used as an antipyretic drug and has been demonstrated to have anti-inflammatory activity. SR is divided into two specifications, "Ku Qin"(KQ) and "Zi Qin"(ZQ), for use against different symptoms(upper energizer heat or lower portion of the triple energizer), according to the theory of traditional Chinese medicine(TCM). However, differences in the efficacies of these two specifications have not been determined. In the present study, we aimed to characterize the differences in the anti-inflammatory activities between KQ and ZQ and to explore how their differences are manifested in lipopolysaccharide(LPS)-induced macrophages. Our results showed that, in RAW264.7 cells(a mouse macrophage cell line derived from ascites), KQ and ZQ displayed anti-inflammatory effects by inhibiting the release of nitric oxide(NO), inducible NOS(iNOS), and nuclear factor-κB(NF-κB) in a dose-dependent manner without distinction. In NR8383 cells(a rat alveolar macrophage cell line), KQ and ZQ displayed similar effects on NO, iNOS, and NF-κB as seen in RAW264.7 cells, but KQ showed a higher inhibition rate for NO and i NOS than that shown by ZQ at the same concentration. These results indicated that there were differences in efficacy between KQ and ZQ in treating lung inflammation. Our findings provided an experimental evidence supporting the different uses of KQ and ZQ in clinic, as noted in ancient herbal records.
文摘The 33 ku protein, prepared from NaCI-treated PSII particles, has shown an single band by SDS-PAGE. After being dialyzed against the low-osmotic medium at 4癈, it has been found that the 33 ku protein degraded into several small fragments. This result suggests that the preparations of 33 ku protein probably contain some latent proteinases. it has also been found, by the 2-D electrophoresis and IEF, that the preparations of 33 ku protein not dialyzed against the low-osmotic medium contain several polypeptides with nearly the same molecular weight but not the same isoelectric point as the 33 ku protein.
文摘Nonhomologous DNA end joining （NHEJ） is the primary pathway for repair of double-strand DNA breaks in human cells and in multicellular eukaryotes. The causes of double-strand breaks often fragment the DNA at the site of damage, resulting in the loss of information there. NHEJ does not restore the lost information and may resect additional nucleotides during the repair process. The ability to repair a wide range of overhang and damage configurations reflects the flexibility of the nuclease, polymerases, and ligase of NHEJ. The flexibility of the individual components also explains the large number of ways in which NHEJ can repair any given pair of DNA ends. The loss of information locally at sites of NHEJ repair may contribute to cancer and aging, but the action by NHEJ ensures that entire segments of chromosomes are not lost.
基金The article is part of the achievements of“A Study of the English Translation of‘The Analects of Confucius’and the Dissemination of Confucian Culture(《论语》的英译与儒家文化对外传播研究),a USST project in 2022.”Its serial number is XJ2022231.
文摘As one of the carriers of Confucian culture,and from ancient times to the present,Lunyu or The Analects or The Analects of Confucius,has been well received by readers at home and abroad,and there are more and more English translations of Lunyu.This article will select some of the vocabulary translations with ancient Chinese characteristics in Lunyu as the object,employ the method of a comparative analysis to explore the cultural connotation behind the text and the dissemination of the translation,and discuss the translator’s translation strategies.Judging from the research on The Analects by scholars from home and abroad and the opening and development of overseas Confucius Institutes,Lunyu has exerted an enlightenment effect in different times.
