Background: The dried roots of Inula helenium L.(IH) and Inula racemosa Hook f.(IR) are used commonly as folk medicine under the name of "tumuxiang(TMX)". Phenolic acid compounds and their derivatives, as ma...Background: The dried roots of Inula helenium L.(IH) and Inula racemosa Hook f.(IR) are used commonly as folk medicine under the name of "tumuxiang(TMX)". Phenolic acid compounds and their derivatives, as main active constituents in IH and IR, exhibit prominent anti-inflammation effect.Objective: To develop a holistic method based on chemical characteristic and anti-inflammation effect for systematically evaluating the quality of twenty-seven TMX samples(including 18 IH samples and 9 IR samples) from different origins.Methods: HPLC fingerprints data of AL(Aucklandia lappa Decne.) whose dried root was similar with HR was added for classification analysis. The HPLC fingerprints of twenty-seven TMX samples and four AL samples were evaluated using hierarchical clustering analysis(HCA) and principle component analysis(PCA). The spectrum-efficacy model between HPLC fingerprints and anti-inflammatory activities was investigated by principal component regression(PCR) and partial least squares(PLS).Results: All samples were successfully divided into three main clusters and peaks 7, 9, 11, 22, 24 and 26 had a primary contribution to classify these medicinal herbs. The results were in accord with the appraisal results of herbs. The spectrum-efficacy relationship results indicated that citric acid, quinic acid, caffeic acid-β-D-glucopyranoside, chlorogenic acid, caffeic acid, 1,3-O-dicaffeoyl quinic acid, tianshic acid and 3β-Hydroxypterondontic acid had main contribution to anti-inflammatory activities.Conclusion: This comprehensive strategy was successfully used for identification of IH, IR and AL, which provided a reliable and adequate theoretical basis for the bioactivity relevant quality standards and studying the material basis of anti-inflammatory effect of TMX.展开更多
The article presents the geobotanical and some bioecological features of species of the genus Inula L., family Asteraceae (Compositae). The water regime, as well as the water holding capacity, transpiration rate and a...The article presents the geobotanical and some bioecological features of species of the genus Inula L., family Asteraceae (Compositae). The water regime, as well as the water holding capacity, transpiration rate and adaptive ability of plants to drought in the arid zone, is considered. The results of studies on the introduction into the culture based on indicators of the osmotic pressure of cell juice are presented.展开更多
本文报道了一种从土木香药材中提取以及纯化倍半萜内酯的方法。乙醇回流提取的方法采用正交试验设计方法[L9（34）],考察了4个因素对产率与内酯浓度的影响,最终确定了乙醇回流提取的最佳方法：药材加10倍量的95%乙醇,加热回流提取1次,2 ...本文报道了一种从土木香药材中提取以及纯化倍半萜内酯的方法。乙醇回流提取的方法采用正交试验设计方法[L9（34）],考察了4个因素对产率与内酯浓度的影响,最终确定了乙醇回流提取的最佳方法：药材加10倍量的95%乙醇,加热回流提取1次,2 h。而硅胶柱层析的最佳纯化方法为：以100~200目的硅胶,1∶3的上样量,径高比为1∶2。其洗脱剂成分为石油醚∶丙酮（100∶3,v/v）,洗脱流速为35 m L/min。采用该方法,异土木香内酯与土木香内酯的提取率大于70%,其纯度大于90%。展开更多
基金the Project of Science and Technology Agency of Gansu(1208RTZA211)Science and Technology Agency of Lanzhou(2013-4-75)the item of scientific and technological researches from Gansu province administration bureau of traditional Chinese medicine(GZK-2014-13)
文摘Background: The dried roots of Inula helenium L.(IH) and Inula racemosa Hook f.(IR) are used commonly as folk medicine under the name of "tumuxiang(TMX)". Phenolic acid compounds and their derivatives, as main active constituents in IH and IR, exhibit prominent anti-inflammation effect.Objective: To develop a holistic method based on chemical characteristic and anti-inflammation effect for systematically evaluating the quality of twenty-seven TMX samples(including 18 IH samples and 9 IR samples) from different origins.Methods: HPLC fingerprints data of AL(Aucklandia lappa Decne.) whose dried root was similar with HR was added for classification analysis. The HPLC fingerprints of twenty-seven TMX samples and four AL samples were evaluated using hierarchical clustering analysis(HCA) and principle component analysis(PCA). The spectrum-efficacy model between HPLC fingerprints and anti-inflammatory activities was investigated by principal component regression(PCR) and partial least squares(PLS).Results: All samples were successfully divided into three main clusters and peaks 7, 9, 11, 22, 24 and 26 had a primary contribution to classify these medicinal herbs. The results were in accord with the appraisal results of herbs. The spectrum-efficacy relationship results indicated that citric acid, quinic acid, caffeic acid-β-D-glucopyranoside, chlorogenic acid, caffeic acid, 1,3-O-dicaffeoyl quinic acid, tianshic acid and 3β-Hydroxypterondontic acid had main contribution to anti-inflammatory activities.Conclusion: This comprehensive strategy was successfully used for identification of IH, IR and AL, which provided a reliable and adequate theoretical basis for the bioactivity relevant quality standards and studying the material basis of anti-inflammatory effect of TMX.
文摘The article presents the geobotanical and some bioecological features of species of the genus Inula L., family Asteraceae (Compositae). The water regime, as well as the water holding capacity, transpiration rate and adaptive ability of plants to drought in the arid zone, is considered. The results of studies on the introduction into the culture based on indicators of the osmotic pressure of cell juice are presented.
基金Major Progress of National New Drug Research(2012ZX09103201-038)
文摘本文报道了一种从土木香药材中提取以及纯化倍半萜内酯的方法。乙醇回流提取的方法采用正交试验设计方法[L9（34）],考察了4个因素对产率与内酯浓度的影响,最终确定了乙醇回流提取的最佳方法：药材加10倍量的95%乙醇,加热回流提取1次,2 h。而硅胶柱层析的最佳纯化方法为：以100~200目的硅胶,1∶3的上样量,径高比为1∶2。其洗脱剂成分为石油醚∶丙酮（100∶3,v/v）,洗脱流速为35 m L/min。采用该方法,异土木香内酯与土木香内酯的提取率大于70%,其纯度大于90%。
