This study was to investigate the effects of dietary supplementation of Bacillus coagulans(BC) and yeast hydrolysates(YH) on growth performance,antioxidant activity,cytokines and intestinal microflora of growing-finis...This study was to investigate the effects of dietary supplementation of Bacillus coagulans(BC) and yeast hydrolysates(YH) on growth performance,antioxidant activity,cytokines and intestinal microflora of growing-finishing pigs.Thirty-six barrows(initial BW=26.87±2.65 kg) were assigned randomly to 3 treatments with 4 replicates,3 pigs per replicate.Pigs in the control group(CON) were fed a basal diet,and the diets for the other 2 groups were the basal diet plus BC at 200 mg/kg and the basal diet plus YH at 3,000 mg/kg.The trial lasted for 104 d.Compared with CON,YH treatment significantly increased average daily gain(ADG) and average daily feed intake(ADFI) during the finishing phase(P <0.05),and significantly enhanced ADG during the overall period(P <0.05).Dietary inclusion of BC tended to increase ADFI during the finishing period(P=0.08).Compared with CON,BC treatment imp roved lysozyme(LZM),complement 3(C3),complement 4(C4),interlenkin-10(IL-10) and total antioxidant capacity(T-AOC) level in serum(P <0.05).Dietary inclusion of YH enhanced the serum IL-10 level(P <0.05) and tended to increase T-AOC level(P=0.06).Dietary inclusion of YH elevated(P <0.05) the number of Lactobacillus and Bacillus in cecal contents of pigs,promoted the populations of Bifidobacterium and Bacillus in colonic contents.Moreover,the BC diet increased(P <0.05) the count of Bifidobacterium in colonic contents.These results indicated that dietary BC supplementation is beneficial to improve the immunity.Dietary YH supplementation promoted the growth performance and the populations of beneficial bacteria in the hindgut of the growing-finishing pigs.展开更多
Lysozymes can hydrolyze bacteria and play an important role in animal digestion and innate immunity. The cDNA of a chicken-type lysozyme gene （Mdlys） was cloned from housefly （Musca domestica）. The 484 bp full-len...Lysozymes can hydrolyze bacteria and play an important role in animal digestion and innate immunity. The cDNA of a chicken-type lysozyme gene （Mdlys） was cloned from housefly （Musca domestica）. The 484 bp full-length cDNA contains a 426 bp open reading frame （ORF） that encodes MdLys of 141 amino acids. Phylogenetic analysis indicated that the MdLys was similar to chicken-type lysozymes. Spatio-temporal expression of Mdlys was analyzed by RT-PCR. The Mdlys transcript can be detected in both midgut and fat body and was expressed at a relatively lower level at the embryo stage. Mdlys mRNA was upregulated 2 h post bacterial challenge, maintained for 2 to 6 h, and slightly declined from 12 to 24 h post-injection. Western blot analysis showed that MdLys was highly expressed in midgut and was also detected in the hemolymph and fat body. MdLys expression was slightly increased in midgut after challenging with Escherichia coli or Staphylococcus aureus. Its expression was also slightly increased in the fat body after challenging with S. aureus, but no obvious change occurred after E. coli challenge. MdLys expression in the hemolymph was not affected by bacterial challenge. In the developmental stages, MdLys expression levels had no obvious change from the first instar to the pupae stage. There was also no variation under 24 h starvation stress. Recombinant MdLys displayed inhibitory activity against Gram-negative and Gram-positive bacteria. Together, these results suggest that MdLys may play an important role in the innate immunity of houseflies.展开更多
基金supported by the grants from the National Key Technology Research and Development Program of the Ministry of Science and Technology of China (2014BAD13B01)the Science and Technology Support Program of Sichuan Province (2014NZ0043).
文摘This study was to investigate the effects of dietary supplementation of Bacillus coagulans(BC) and yeast hydrolysates(YH) on growth performance,antioxidant activity,cytokines and intestinal microflora of growing-finishing pigs.Thirty-six barrows(initial BW=26.87±2.65 kg) were assigned randomly to 3 treatments with 4 replicates,3 pigs per replicate.Pigs in the control group(CON) were fed a basal diet,and the diets for the other 2 groups were the basal diet plus BC at 200 mg/kg and the basal diet plus YH at 3,000 mg/kg.The trial lasted for 104 d.Compared with CON,YH treatment significantly increased average daily gain(ADG) and average daily feed intake(ADFI) during the finishing phase(P <0.05),and significantly enhanced ADG during the overall period(P <0.05).Dietary inclusion of BC tended to increase ADFI during the finishing period(P=0.08).Compared with CON,BC treatment imp roved lysozyme(LZM),complement 3(C3),complement 4(C4),interlenkin-10(IL-10) and total antioxidant capacity(T-AOC) level in serum(P <0.05).Dietary inclusion of YH enhanced the serum IL-10 level(P <0.05) and tended to increase T-AOC level(P=0.06).Dietary inclusion of YH elevated(P <0.05) the number of Lactobacillus and Bacillus in cecal contents of pigs,promoted the populations of Bifidobacterium and Bacillus in colonic contents.Moreover,the BC diet increased(P <0.05) the count of Bifidobacterium in colonic contents.These results indicated that dietary BC supplementation is beneficial to improve the immunity.Dietary YH supplementation promoted the growth performance and the populations of beneficial bacteria in the hindgut of the growing-finishing pigs.
基金supported by the National High Technology Research and Development Program of China (863 Program) (No. 2007AA09Z425 and 2006AA100311)the Ph.D. Program Foundation of the Ministry of Education of China (No. 20060422034).
文摘Lysozymes can hydrolyze bacteria and play an important role in animal digestion and innate immunity. The cDNA of a chicken-type lysozyme gene （Mdlys） was cloned from housefly （Musca domestica）. The 484 bp full-length cDNA contains a 426 bp open reading frame （ORF） that encodes MdLys of 141 amino acids. Phylogenetic analysis indicated that the MdLys was similar to chicken-type lysozymes. Spatio-temporal expression of Mdlys was analyzed by RT-PCR. The Mdlys transcript can be detected in both midgut and fat body and was expressed at a relatively lower level at the embryo stage. Mdlys mRNA was upregulated 2 h post bacterial challenge, maintained for 2 to 6 h, and slightly declined from 12 to 24 h post-injection. Western blot analysis showed that MdLys was highly expressed in midgut and was also detected in the hemolymph and fat body. MdLys expression was slightly increased in midgut after challenging with Escherichia coli or Staphylococcus aureus. Its expression was also slightly increased in the fat body after challenging with S. aureus, but no obvious change occurred after E. coli challenge. MdLys expression in the hemolymph was not affected by bacterial challenge. In the developmental stages, MdLys expression levels had no obvious change from the first instar to the pupae stage. There was also no variation under 24 h starvation stress. Recombinant MdLys displayed inhibitory activity against Gram-negative and Gram-positive bacteria. Together, these results suggest that MdLys may play an important role in the innate immunity of houseflies.
