Objective Chinese allergic subjects have high levels of sensitization to house dust mite （HDM） and other indoor allergens. This study quantifies common indoor allergen levels in Chinese households. Methods Dust samp...Objective Chinese allergic subjects have high levels of sensitization to house dust mite （HDM） and other indoor allergens. This study quantifies common indoor allergen levels in Chinese households. Methods Dust samples were collected from nine cities. Major allergens Der p 1 and Der f I from Dermatophagoides pteronyssinus and D. farinae, and specific antigens of Blomia tropicalis, Tyrophagus putrescentiae, Acarus siro, and cockroach species Blattella germanica and Periplaneta americana were measured by ELISA.Results HDM allergens were found in dust samples from bedding in 95% of the Chinese households. The median levels varied from 〈0.006 to 9.2 μg/g of dust, depending on the city. The percentages of households having HDM allergen levels associated with the risk of developing allergy sensitization and asthma were 65% and 25%, respectively. Specific antigens of the storage mite and cockroach were only found in samples from the southern and tropical regions of China. Levels of mite allergens were generally higher in samples from bedding compared to samples from the living room, even for storage mites, whereas levels of cockroach antigens were higher in the living room samples.Conclusion HDM allergens are present in bedding dust samples from most Chinese households. Cities in southern and central China have relatively high levels of HDM major allergens compared to cities in northern and western China. Antigens of storage mites and cockroaches are not as common as HDM allergens.展开更多
Background Subcutaneous specific immunotherapy has been demonstrated to be capable of inducing T-cell regulatory response.Interleukin-10 （IL-10） plays a crucial role in inducing allergen-specific tolerance.However t...Background Subcutaneous specific immunotherapy has been demonstrated to be capable of inducing T-cell regulatory response.Interleukin-10 （IL-10） plays a crucial role in inducing allergen-specific tolerance.However the reports of the changes of IL-10 in house dust mite （HDM）-specific immunotherapy were varied.The aim of this study was to evaluate the function of IL-10-secreting regulatory T cells in asthma children successfully treated with HDM immunotherapy.Methods Peripheral blood mononuclear cells （PBMCs） were isolated from 27 patients following 1.5--2 years of HDM-specific immunotherapy （SIT, SIT group） and from 27 matched treated asthmatic children allergic to HDM （asthmagroup）.After 48 hours of in vitro stimulation with HDM extracts, IL-10-secreting regulatory T cells were measured by four colour flow cytometry.Sera were tested for allergen-specific IgG4 and IgE using the Immuno CAP 100 assay.Results PBMCs from children undergoing immunotherapy following HDM extracts stimuli produced significantly more IL-10 compared with the asthma group.The frequency of iTreg cells and aTreg cells increased in SIT group after HDM stimulation, while it was not affected in the asthma group.Among the iTreg cells and aTreg cells, the frequency of CD4＋CD25-Foxp3-IL-10＋ Treg cells increased the most which was 2 times higher than that in unstimulated cultures in SIT group.The levels of HDM-specific IgG4 of SIT group was significiently higher compared with asthma group, but there was no correlation of the levels of HDM-specific IgG4 and IL-10 secreting Treg cells.Conclusions HDM-specific immunotherapy can successfully upregulate the frequency of IL-10-secreting Treg cells.CD4＋CD25-Foxp3-IL-10＋ Treg cells may play a key role in inducing the immune tolerance in HDM-specific immunotherapy.展开更多
Background： Group 2 innate lymphoid cells （ILC2s） are regarded as a novel population of lineage-negative cells that induce innate Type 2 responses by producing the critical Th2-type cytokines interleukin （IL）-5 a...Background： Group 2 innate lymphoid cells （ILC2s） are regarded as a novel population of lineage-negative cells that induce innate Type 2 responses by producing the critical Th2-type cytokines interleukin （IL）-5 and IL-13. ILC2s as key players in the development of allergic rhinitis （AR） have been proved, however, the effect of subcutaneous immunotherapy （SCIT） with dermatophagoides pteronyssinus extract （Der p-SCIT） on ILC2s in AR patients is not clear. This study aimed to investigate the response of ILC2s of peripheral blood in house dust mites （HDM）-sensitized Chinese patients with AR who received SCIT with Der P extract. Methods： Seven healthy controls without symptoms of AR who had negative reactions to any of the allergens from skin-prick testing, nine patients diagnosed with persistent AR according to the Allergic Rhinitis and its Impact on Asthma （ARIA） guidelines, and 24 AR patients who received Der p-SCIT for 1.0-3.5 years were recruited for the study. ILC2s in the peripheral blood were evaluated using flow cytometry. The severity of their symptoms of all participants was rated based on the Total 5 symptom score. Results： Among 40 participants, 9 AR patients were assigned to the untreated group, 24 AR patients receiving Der p-SCIT were assigned to the immunotherapy group, and 7 healthy controls without symptoms of AR were assigned to healthy control group. The mean Total 5 symptom score of immunotherapy group was significantly lower than that of untreated group （4.3 ± 1.4 vs. 10.1 ± 2.5, P 〈 0.001 ）. Similarly, the levels of ILC2s in the peripheral blood ofimmunotherapy group were significantly reduced compared with that in untreated group （P 〈 0.001 ）, but were not significantly different from healthy controls （P = 0.775）. Further subgroup analysis based on the duration of SCIT therapy （ 1.0-2.0 years [SCIT1-2], 2.0-3.0 years [SCIT2_3], and 3.0-3.5 years [SCIT3_3.5]） showed that the percentage of ILC2s was not significantly different between SCIT1-展开更多
目的 探讨花粉症患者在发作期和非发作期外周血Th17/调节性T细胞（Treg）平衡变化及其临床意义.方法 纳入13例花粉症患者、10例尘螨过敏哮喘患者以及10例健康儿童,分别在发作期和非发作期采用流式细胞术检测外周血Th17/Treg平衡变化,EL...目的 探讨花粉症患者在发作期和非发作期外周血Th17/调节性T细胞（Treg）平衡变化及其临床意义.方法 纳入13例花粉症患者、10例尘螨过敏哮喘患者以及10例健康儿童,分别在发作期和非发作期采用流式细胞术检测外周血Th17/Treg平衡变化,ELISA检测血清中IL-17、IL-10和TGF-β水平.结果 （1）在发作期内,花粉症组外周血Th17细胞[（3.4±2.4）%]显著高于尘螨过敏哮喘组[（2.1±1.6）%]、健康对照组[（0.5±0.3）%]（P均〈0.05）;花粉症组[（2.1±1.3）%]、尘螨过敏哮喘组[（3.6±1.9）%]外周血Treg细胞水平均显著低于健康对照组[（5.5±2.8）%],差异具有统计学意义（P均〈0.05）.在非发作期,花粉症组患者外周血Th17细胞[（3.0±1.9）% vs （3.4±2.4）%,P〈0.05]、Th17/Treg比例[（1.4±1.0）% vs （1.7±1.5）%, P〈0.05]均降低,而Treg水平升高[（2.4±1.6）% vs （2.1±1.3）%,P〈0.05].未观察到尘螨过敏哮喘组患者在非发作期外周血Th17细胞、Treg以及Th17/Treg比例发生变化. （2）相关性分析：发作期内花粉症组外周血Th17/Treg比例与呼出气一氧化氮（FeNO）水平（r=0.321,P〈0.05）、外周血嗜酸性粒细胞计数存在正相关关系（r=0.198,P〈0.05）.结论 花粉症患者在发作期内存在Th17/Treg免疫失衡的病理状态,这可能是导致其临床症状急性发作的重要机制之一.展开更多
文摘Objective Chinese allergic subjects have high levels of sensitization to house dust mite （HDM） and other indoor allergens. This study quantifies common indoor allergen levels in Chinese households. Methods Dust samples were collected from nine cities. Major allergens Der p 1 and Der f I from Dermatophagoides pteronyssinus and D. farinae, and specific antigens of Blomia tropicalis, Tyrophagus putrescentiae, Acarus siro, and cockroach species Blattella germanica and Periplaneta americana were measured by ELISA.Results HDM allergens were found in dust samples from bedding in 95% of the Chinese households. The median levels varied from 〈0.006 to 9.2 μg/g of dust, depending on the city. The percentages of households having HDM allergen levels associated with the risk of developing allergy sensitization and asthma were 65% and 25%, respectively. Specific antigens of the storage mite and cockroach were only found in samples from the southern and tropical regions of China. Levels of mite allergens were generally higher in samples from bedding compared to samples from the living room, even for storage mites, whereas levels of cockroach antigens were higher in the living room samples.Conclusion HDM allergens are present in bedding dust samples from most Chinese households. Cities in southern and central China have relatively high levels of HDM major allergens compared to cities in northern and western China. Antigens of storage mites and cockroaches are not as common as HDM allergens.
文摘Background Subcutaneous specific immunotherapy has been demonstrated to be capable of inducing T-cell regulatory response.Interleukin-10 （IL-10） plays a crucial role in inducing allergen-specific tolerance.However the reports of the changes of IL-10 in house dust mite （HDM）-specific immunotherapy were varied.The aim of this study was to evaluate the function of IL-10-secreting regulatory T cells in asthma children successfully treated with HDM immunotherapy.Methods Peripheral blood mononuclear cells （PBMCs） were isolated from 27 patients following 1.5--2 years of HDM-specific immunotherapy （SIT, SIT group） and from 27 matched treated asthmatic children allergic to HDM （asthmagroup）.After 48 hours of in vitro stimulation with HDM extracts, IL-10-secreting regulatory T cells were measured by four colour flow cytometry.Sera were tested for allergen-specific IgG4 and IgE using the Immuno CAP 100 assay.Results PBMCs from children undergoing immunotherapy following HDM extracts stimuli produced significantly more IL-10 compared with the asthma group.The frequency of iTreg cells and aTreg cells increased in SIT group after HDM stimulation, while it was not affected in the asthma group.Among the iTreg cells and aTreg cells, the frequency of CD4＋CD25-Foxp3-IL-10＋ Treg cells increased the most which was 2 times higher than that in unstimulated cultures in SIT group.The levels of HDM-specific IgG4 of SIT group was significiently higher compared with asthma group, but there was no correlation of the levels of HDM-specific IgG4 and IL-10 secreting Treg cells.Conclusions HDM-specific immunotherapy can successfully upregulate the frequency of IL-10-secreting Treg cells.CD4＋CD25-Foxp3-IL-10＋ Treg cells may play a key role in inducing the immune tolerance in HDM-specific immunotherapy.
基金This work was supported by grants from the Program for Changjiang Scholars and Innovative Research Team （No. IRT13082）, the 12th Five-Year Science and Technology Support Project （No. 2014BAI07B04）, the National Science Fund for the Major International Joint Research Program （No. 81420108009）, National Natural Science Foundation of China （No. 81100704, 81441029, 81441031, and 81570894）, Beijing Natural Science Foundation （No. 7131006）, Beijing Municipal Administration of Hospitals Clinical Medicine Development of Special Funding Support （No. ZYLX201310）, the Capital Health Research and Development of Special （No. 2011-1017-06）, Specialized Research Fund for the Doctoral Program of Higher Education of China （No. 20111107120004）, the Special Fund of Sanitation Elite Reconstruction of Beijing （No. 2009-2-007）, Beijing Municipal Administration of Hospitals＇ Mission Plan （No. SML20150203）, and Beijing Health Bureau Program for High Level Talents （No. 2009-2-007, 2011-3-039, 2011-3-043, and 2014-3-018）.
文摘Background： Group 2 innate lymphoid cells （ILC2s） are regarded as a novel population of lineage-negative cells that induce innate Type 2 responses by producing the critical Th2-type cytokines interleukin （IL）-5 and IL-13. ILC2s as key players in the development of allergic rhinitis （AR） have been proved, however, the effect of subcutaneous immunotherapy （SCIT） with dermatophagoides pteronyssinus extract （Der p-SCIT） on ILC2s in AR patients is not clear. This study aimed to investigate the response of ILC2s of peripheral blood in house dust mites （HDM）-sensitized Chinese patients with AR who received SCIT with Der P extract. Methods： Seven healthy controls without symptoms of AR who had negative reactions to any of the allergens from skin-prick testing, nine patients diagnosed with persistent AR according to the Allergic Rhinitis and its Impact on Asthma （ARIA） guidelines, and 24 AR patients who received Der p-SCIT for 1.0-3.5 years were recruited for the study. ILC2s in the peripheral blood were evaluated using flow cytometry. The severity of their symptoms of all participants was rated based on the Total 5 symptom score. Results： Among 40 participants, 9 AR patients were assigned to the untreated group, 24 AR patients receiving Der p-SCIT were assigned to the immunotherapy group, and 7 healthy controls without symptoms of AR were assigned to healthy control group. The mean Total 5 symptom score of immunotherapy group was significantly lower than that of untreated group （4.3 ± 1.4 vs. 10.1 ± 2.5, P 〈 0.001 ）. Similarly, the levels of ILC2s in the peripheral blood ofimmunotherapy group were significantly reduced compared with that in untreated group （P 〈 0.001 ）, but were not significantly different from healthy controls （P = 0.775）. Further subgroup analysis based on the duration of SCIT therapy （ 1.0-2.0 years [SCIT1-2], 2.0-3.0 years [SCIT2_3], and 3.0-3.5 years [SCIT3_3.5]） showed that the percentage of ILC2s was not significantly different between SCIT1-
文摘目的 探讨花粉症患者在发作期和非发作期外周血Th17/调节性T细胞（Treg）平衡变化及其临床意义.方法 纳入13例花粉症患者、10例尘螨过敏哮喘患者以及10例健康儿童,分别在发作期和非发作期采用流式细胞术检测外周血Th17/Treg平衡变化,ELISA检测血清中IL-17、IL-10和TGF-β水平.结果 （1）在发作期内,花粉症组外周血Th17细胞[（3.4±2.4）%]显著高于尘螨过敏哮喘组[（2.1±1.6）%]、健康对照组[（0.5±0.3）%]（P均〈0.05）;花粉症组[（2.1±1.3）%]、尘螨过敏哮喘组[（3.6±1.9）%]外周血Treg细胞水平均显著低于健康对照组[（5.5±2.8）%],差异具有统计学意义（P均〈0.05）.在非发作期,花粉症组患者外周血Th17细胞[（3.0±1.9）% vs （3.4±2.4）%,P〈0.05]、Th17/Treg比例[（1.4±1.0）% vs （1.7±1.5）%, P〈0.05]均降低,而Treg水平升高[（2.4±1.6）% vs （2.1±1.3）%,P〈0.05].未观察到尘螨过敏哮喘组患者在非发作期外周血Th17细胞、Treg以及Th17/Treg比例发生变化. （2）相关性分析：发作期内花粉症组外周血Th17/Treg比例与呼出气一氧化氮（FeNO）水平（r=0.321,P〈0.05）、外周血嗜酸性粒细胞计数存在正相关关系（r=0.198,P〈0.05）.结论 花粉症患者在发作期内存在Th17/Treg免疫失衡的病理状态,这可能是导致其临床症状急性发作的重要机制之一.
