Harringtonine (HT), a kind of anticancer drug isolated from Chinese herb-Cephalotaxus hainanensis Li, can induce apoptosis in promyelocytic leukemia HL-60 cells. With both two-photon laser scanning microscopy and conf...Harringtonine (HT), a kind of anticancer drug isolated from Chinese herb-Cephalotaxus hainanensis Li, can induce apoptosis in promyelocytic leukemia HL-60 cells. With both two-photon laser scanning microscopy and confocal laser scanning microscopy in combination with the fluores-cent probe Hoechst 33342, tetramethyrhodamine ethyl ester (TMRE) and Fluo 3-AM, we simulta-neously observed HT-induced changes in nuclear morphology, mitochondrial membrane potential and intracellular calcium concentration ([Ca2+]i) in HL-60 cells, and developed a real-time, sensitive and invasive method for simultaneous multi-parameter observation of drug- treating living cells at the level of single cell.展开更多
The effect of Ca 2+ on HT induced apoptosis in HL 60 cells was examined. Characteristics of apoptosis induced by harringtonine (HT) or campothecin (CAM) could not be abolished by extracellular calcium chelator EGTA; h...The effect of Ca 2+ on HT induced apoptosis in HL 60 cells was examined. Characteristics of apoptosis induced by harringtonine (HT) or campothecin (CAM) could not be abolished by extracellular calcium chelator EGTA; however, an intracellular Ca 2+ chelator BAPTA AM could block HT or CAM induced HL 60 cell apoptosis. Requirement of intracellular calcium for HT\|induced apoptosis is further supported by the fact that intracellular Ca 2+ depleted HL 60 cells could not undergo HT induced apoptosis. No significant increase of intracellular Ca 2+ was found after HT treatment. By using video enhancement contrast microscopy (VEC), the dynamic changes of intracellular calcium distribution over the whole period of apoptosis in the same individual cell were detected. The results demonstrated the movement of Ca 2+ from cytosol to nucleus after initiation of apoptosis by treatment with HT.展开更多
基金the Tsinghua University Foundation for Basic Research and the Chinese Postdoctoral Foundation.
文摘Harringtonine (HT), a kind of anticancer drug isolated from Chinese herb-Cephalotaxus hainanensis Li, can induce apoptosis in promyelocytic leukemia HL-60 cells. With both two-photon laser scanning microscopy and confocal laser scanning microscopy in combination with the fluores-cent probe Hoechst 33342, tetramethyrhodamine ethyl ester (TMRE) and Fluo 3-AM, we simulta-neously observed HT-induced changes in nuclear morphology, mitochondrial membrane potential and intracellular calcium concentration ([Ca2+]i) in HL-60 cells, and developed a real-time, sensitive and invasive method for simultaneous multi-parameter observation of drug- treating living cells at the level of single cell.
文摘The effect of Ca 2+ on HT induced apoptosis in HL 60 cells was examined. Characteristics of apoptosis induced by harringtonine (HT) or campothecin (CAM) could not be abolished by extracellular calcium chelator EGTA; however, an intracellular Ca 2+ chelator BAPTA AM could block HT or CAM induced HL 60 cell apoptosis. Requirement of intracellular calcium for HT\|induced apoptosis is further supported by the fact that intracellular Ca 2+ depleted HL 60 cells could not undergo HT induced apoptosis. No significant increase of intracellular Ca 2+ was found after HT treatment. By using video enhancement contrast microscopy (VEC), the dynamic changes of intracellular calcium distribution over the whole period of apoptosis in the same individual cell were detected. The results demonstrated the movement of Ca 2+ from cytosol to nucleus after initiation of apoptosis by treatment with HT.
