OBJECTIVE:To investigate pharmacodynamic effects of modified Gexiazhuyu decoction(MGXZYD)and explore the underlying mechanism in the treatment of chronic salpingitis METHODS:Chronic salpingitis model rats were firstly...OBJECTIVE:To investigate pharmacodynamic effects of modified Gexiazhuyu decoction(MGXZYD)and explore the underlying mechanism in the treatment of chronic salpingitis METHODS:Chronic salpingitis model rats were firstly constructed and the blood was collected to detect the whole blood viscosity and plasma viscosity.Rat oviduct were collected to evaluate the macroscopic damage and the pathological injury and fibrosis of oviduct by hematoxylin-eosin(HE)and Masson staining.Elisa assay was to detect the production interleukin-1β(IL-1β)in serum and collagen I(COL-1),matrix metalloprotein 9(MMP-9),tissue inhibitor of metalloproteinases 1(TIMP-1)in oviduct tissue.And immunohistochemical staining with MMP-9 and TIMP-1 in oviduct tissue were examined.Western blot was used to detect the expressions of p38 mitogen-activated protein kinases(p38MAPK),phosphop38MPAK(p-p38MPAK),transforming growth factor-β1(TGF-β1)in oviduct.The expression ofα-smooth muscle actin(α-SMA),p-p38MPAK,in oviduct tissue were detected by immunofluorescence method.The m RNA of p-p38MAPK,α-SMA,COL-1,MMP-9,TIMP-1 was measured by reverse transcription-polymerase chain reaction.RESULTS:Rats administrated with MGXZYD demonstrated decreased the whole blood viscosity and plasma viscosity.MGXZYD obviously improved the tubal wall thickening,swelling and pelvic adhesion.And HE and Masson staining showed MGXZYD improved the pathological injury and fibrosis of oviduct.The results of MTT assay and flow cytometry indicated that MGXZYD could decreased the NIN-3T3 cells viability and improved the apoptosis.Besides,MGXZYD inhibited the protein and/or m RNA of TGF-β1,IL-1β,COL-1,α-SMA,pp38MAPK expressions and increased the production of MMP-9/TIMP-1.CONCLUSION:MGXZYD could prevent the progression of chronic salpingitis by inhibited the fibrocyte and inflammation which via inhibited the p38 MAPK signaling pathway.展开更多
目的:基于网络药理学方法,探讨膈下逐瘀汤治疗炎症性肠病的作用机制。方法:借助中药系统药理学数据库和分析平台、中药系统药理学成分分析平台数据库,检索膈下逐瘀汤中药物的化学成分和作用靶点;通过UniProt、Malacards和PubMed等数据库...目的:基于网络药理学方法,探讨膈下逐瘀汤治疗炎症性肠病的作用机制。方法:借助中药系统药理学数据库和分析平台、中药系统药理学成分分析平台数据库,检索膈下逐瘀汤中药物的化学成分和作用靶点;通过UniProt、Malacards和PubMed等数据库,查询疾病靶点;运用Cytoscape 3.2.1软件构建“成分-靶点”网络,并通过京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)通路富集分析及基因本体(gene ontology,GO)富集分析,探究其作用机制。结果:共筛选出257种药物有效成分,39个生物学作用靶点,其中175个活性成分参与作用靶点的一级交互网络构建。KEGG通路富集分析结果主要为肿瘤坏死因子信号通路、炎症性肠病通路、Toll样受体通路、破骨细胞分化通路及细胞因子-细胞因子受体通路;GO富集分析涉及的生物学过程作用主要为脂多糖应答、菌源性的分子识别、活性氧代谢过程、细胞对生物刺激反应及对肿瘤坏死因子应答反应等过程;分子功能主要为细胞因子受体结合、细胞因子活性、生长因子受体结合、蛋白磷酸酶结合及过氧化物酶活性等;生物学靶点主要富集在细胞间黏附分子-1、白细胞介素1β、肿瘤坏死因子α、转化生长因子β1及白细胞介素6等。结论:膈下逐瘀汤可通过多成分、多靶点、多通路治疗炎症性肠病,其作用的物质基础及机制仍需系统研究。展开更多
基金Supported by the Scientific Research Project of Heilongjiang Administration of Traditional Chinese Medicine:Clinical Study on the Treatment of Tubal Inflammatory Infertility with Gexia Zhuyu Decoction Combined with Hysteroscopic Tubal Fluid Passage(ZHY18-087)Natural Science Foundation of Heilongjiang Province:Study on the Effect of"Activating Blood Circulation and Removing Stasis,Activating Qi and Relieving Pain"on Salpingitis and Infertility Rat Model(H2015026)。
文摘OBJECTIVE:To investigate pharmacodynamic effects of modified Gexiazhuyu decoction(MGXZYD)and explore the underlying mechanism in the treatment of chronic salpingitis METHODS:Chronic salpingitis model rats were firstly constructed and the blood was collected to detect the whole blood viscosity and plasma viscosity.Rat oviduct were collected to evaluate the macroscopic damage and the pathological injury and fibrosis of oviduct by hematoxylin-eosin(HE)and Masson staining.Elisa assay was to detect the production interleukin-1β(IL-1β)in serum and collagen I(COL-1),matrix metalloprotein 9(MMP-9),tissue inhibitor of metalloproteinases 1(TIMP-1)in oviduct tissue.And immunohistochemical staining with MMP-9 and TIMP-1 in oviduct tissue were examined.Western blot was used to detect the expressions of p38 mitogen-activated protein kinases(p38MAPK),phosphop38MPAK(p-p38MPAK),transforming growth factor-β1(TGF-β1)in oviduct.The expression ofα-smooth muscle actin(α-SMA),p-p38MPAK,in oviduct tissue were detected by immunofluorescence method.The m RNA of p-p38MAPK,α-SMA,COL-1,MMP-9,TIMP-1 was measured by reverse transcription-polymerase chain reaction.RESULTS:Rats administrated with MGXZYD demonstrated decreased the whole blood viscosity and plasma viscosity.MGXZYD obviously improved the tubal wall thickening,swelling and pelvic adhesion.And HE and Masson staining showed MGXZYD improved the pathological injury and fibrosis of oviduct.The results of MTT assay and flow cytometry indicated that MGXZYD could decreased the NIN-3T3 cells viability and improved the apoptosis.Besides,MGXZYD inhibited the protein and/or m RNA of TGF-β1,IL-1β,COL-1,α-SMA,pp38MAPK expressions and increased the production of MMP-9/TIMP-1.CONCLUSION:MGXZYD could prevent the progression of chronic salpingitis by inhibited the fibrocyte and inflammation which via inhibited the p38 MAPK signaling pathway.
文摘目的:基于网络药理学方法,探讨膈下逐瘀汤治疗炎症性肠病的作用机制。方法:借助中药系统药理学数据库和分析平台、中药系统药理学成分分析平台数据库,检索膈下逐瘀汤中药物的化学成分和作用靶点;通过UniProt、Malacards和PubMed等数据库,查询疾病靶点;运用Cytoscape 3.2.1软件构建“成分-靶点”网络,并通过京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)通路富集分析及基因本体(gene ontology,GO)富集分析,探究其作用机制。结果:共筛选出257种药物有效成分,39个生物学作用靶点,其中175个活性成分参与作用靶点的一级交互网络构建。KEGG通路富集分析结果主要为肿瘤坏死因子信号通路、炎症性肠病通路、Toll样受体通路、破骨细胞分化通路及细胞因子-细胞因子受体通路;GO富集分析涉及的生物学过程作用主要为脂多糖应答、菌源性的分子识别、活性氧代谢过程、细胞对生物刺激反应及对肿瘤坏死因子应答反应等过程;分子功能主要为细胞因子受体结合、细胞因子活性、生长因子受体结合、蛋白磷酸酶结合及过氧化物酶活性等;生物学靶点主要富集在细胞间黏附分子-1、白细胞介素1β、肿瘤坏死因子α、转化生长因子β1及白细胞介素6等。结论:膈下逐瘀汤可通过多成分、多靶点、多通路治疗炎症性肠病,其作用的物质基础及机制仍需系统研究。
