目的:应用寡核苷酸基因芯片技术,研究血瘀证患者差异基因表达谱。方法:16例血瘀证患者经冠状动脉造影诊断后,分为冠心病血瘀证组和非冠心病血瘀证组,每组8例;并选年龄和性别相匹配的8名健康人为健康对照组。抽取静脉血,分离白细胞,抽提R...目的:应用寡核苷酸基因芯片技术,研究血瘀证患者差异基因表达谱。方法:16例血瘀证患者经冠状动脉造影诊断后,分为冠心病血瘀证组和非冠心病血瘀证组,每组8例;并选年龄和性别相匹配的8名健康人为健康对照组。抽取静脉血,分离白细胞,抽提RNA,质控芯片(Test3芯片)对样本质量进行检测,然后与Affymetrix U133 Plus 2.0芯片进行杂交,通过扫描和软件分析,比较冠心病血瘀证组、非冠心病血瘀证组与健康对照组的基因表达谱,筛选血瘀证相关差异基因,并进一步进行基因本体(Gene Ontology,GO)和通路分析,使用实时荧光定量逆转录聚合酶链反应法对目标基因进行验证。结果:通过差异基因筛选,与血瘀证相关的差异基因共有48个,其中上调基因26个,下调基因22个;通过GO分析,其中与炎症免疫相关的基因有5个,占10.4%;通路分析结果显示,有意义的10条通路中有5个涉及炎症和免疫反应。经实时荧光定量逆转录聚合酶链反应验证了基因芯片准确可靠。结论:血瘀证基因表达谱研究显示了炎症和免疫相关基因的比例和显著性优势,说明炎症和免疫反应在一定程度上介导了血瘀证的发生发展。展开更多
AIM： To investigate alternative or subordinate pathways involved in colorectal tumorigenesis and tumor growth, possibly determining at-risk populations and predicting responses to treatment. METHODS： Using microarra...AIM： To investigate alternative or subordinate pathways involved in colorectal tumorigenesis and tumor growth, possibly determining at-risk populations and predicting responses to treatment. METHODS： Using microarray gene-expression analysis, we analyzed patterns of gene expression relative to canonical molecular changes and clinicopathological features in 84 sporadic colorectal cancer patients, standardized by tumor location. Subsets of differentially expressed genes were confirmed by real-time reverse-transcript polymerase chain reaction （RT-PCR）. RESULTS： The largest number of genes identified as being differentially expressed was by tumor location, and the next largest number by lymphovascular or neural invasion of tumor cells and by mismatch repair （NMR） defects. Amongst biological processes, the immune response was significantly implicated in entire molecular changes observed during colorectal tumorigenesis （P 〈 0.001）. Amongst 47 differentially expressed genes, seven （PISD, NIBP, BAI2, STOML1, MRPL21, MRPL16, and MKKS） were newly found to correlate with tumorigenesis and tumor growth. Most location-associated molecular changes had distinct effects on gene expression, but the effects of the latter were sometimes contradictory. CONCLUSION： We show that several differentially expressed genes were associated with canonical molecular changes in sporadic colorectal cancers, possibly constituting alternative or subordinate pathways of tumorigenesis. As tumor location was the dominant factor influencing differential gene expression, location-specific analysis may identify location-associated pathways and enhance the accuracy of class prediction.展开更多
目的观察淫羊藿总黄酮(epi medium flavonoids,EF)对皮质酮大鼠肾上腺皮质再生的调控作用。方法采用碘化丙啶染色流式细胞术检测细胞周期分布和细胞凋亡率;用末端转移酶介导的X-dUTP缺口末端标记(TUNEL)法原位显示凋亡细胞;用基因芯片...目的观察淫羊藿总黄酮(epi medium flavonoids,EF)对皮质酮大鼠肾上腺皮质再生的调控作用。方法采用碘化丙啶染色流式细胞术检测细胞周期分布和细胞凋亡率;用末端转移酶介导的X-dUTP缺口末端标记(TUNEL)法原位显示凋亡细胞;用基因芯片技术检测mRNA表达。结果模型组与正常对照组比较,肾上腺细胞G0/G1期阻滞,凋亡率明显升高(P<0·05);EF干预后G0/G1期比例下降,G2/M期比例显著上升(P<0·01),凋亡率下降(P<0·05)。TUNEL法原位细胞凋亡检测显示正常对照组、模型组、治疗组凋亡阳性细胞数分别为(4·67±1·53)、(70·67±9·29)、(18·67±7·64)个(n=3)。皮质酮主要抑制肾上腺的基因表达,其中7个促进细胞周期的基因如V-ras、V-jun均下调。EF治疗后,肾上腺基因表达以上调为主,其中有6个促进细胞周期基因、1个抗凋亡基因,与肾上腺皮质细胞再生密切相关的上游分子IGF-Ⅱ及其受体IGFR,FGF7及其受体FGFR基因,7个参与类固醇生物合成的基因均上调。结论EF通过促进肾上腺皮质细胞增殖、抑制其细胞凋亡、促进类固醇生物合成,从而促进肾上腺皮质再生是EF在激素撤除过程中发挥保护肾上腺皮质功能的机制。展开更多
OBJECTIVE: To investigate genes involved in cancer metastasis, mRNA differential display was used to compare the levels of gene expression in two cell sublines derived from human giant cell carcinoma of lung (PG) whic...OBJECTIVE: To investigate genes involved in cancer metastasis, mRNA differential display was used to compare the levels of gene expression in two cell sublines derived from human giant cell carcinoma of lung (PG) which had different metastatic potentials. METHODS: Using in vivo tumorigenicity and a spontaneous metastasis assay in nude mice, two sublines (BE1, LH7) from human giant cell carcinoma of lung (PG) with different metastatic potentials were isolated and characterized. mRNA differential display was used to compare the levels of gene expression between them and the obtained results were confirmed by Northern hybridization. RESULTS: One differentially expressed band was nearly identical (99% homology) to Ras-GTPase-Activating protein SH3 domain binding protein (G3BP). G3BP displayed a strong expression in LH7 (non-metastatic in recipient nude mice) and a very weak expression in BE1 (100% metastatic frequency). The same different expression level of G3BP was detected in Northern hybridization with another panel of cell sublines with different metastatic potentials (established in our lab) derived from human prostate carcinoma cell line PC-3M. CONCLUSION: Our results indicate that G3BP was implicated in cancer metastasis because of its differential expressions in the two panels of cell sublines with different metastatic potentials.展开更多
目的在整体动物水平研究淫羊藿总黄酮(EF)延缓衰老的效果和分子机制。方法采用果蝇寿命实验,观察EF延长果蝇寿命的效果;采用果蝇全基因组寡核苷酸芯片研究EF延缓衰老的机制。结果空白对照组、V it E组、EF低剂量组及高剂量组平均寿命分...目的在整体动物水平研究淫羊藿总黄酮(EF)延缓衰老的效果和分子机制。方法采用果蝇寿命实验,观察EF延长果蝇寿命的效果;采用果蝇全基因组寡核苷酸芯片研究EF延缓衰老的机制。结果空白对照组、V it E组、EF低剂量组及高剂量组平均寿命分别为(34.90±23.04),(35.25±23.41),(41.48±25.00)和(46.23±26.20)d,高低剂量EF均显著延长果蝇的平均寿命(P<0.01或P<0.05),高剂量EF提高平均寿命32.46%。老年果蝇呼吸链相关酶、蛋白分解酶、促凋亡基因上调2倍以上,而自由基清除酶、抗凋亡基因、内分泌免疫相关基因下调2倍以上。EF能下调老年果蝇呼吸链相关酶而上调自由基清除酶基因,下调蛋白分解酶基因,能上调促进细胞周期、抗凋亡、免疫相关基因。结论老年果蝇存在自由基产生过多而清除减少、凋亡加速、蛋白溶解过度、内分泌免疫功能低下的内在机制,EF通过减少自由基的产生,增加自由基的清除,减少蛋白分解,促进细胞增殖,恢复免疫功能、对抗凋亡而发挥延缓衰老的功效。展开更多
目的：探讨寻常性银屑病患者皮损及外周血miRNA表达谱系，寻找表达一致的miRNA。方法用基因芯片技术筛选17例银屑病皮损和外周血miRNA，通过验证筛选出差异性miRNA，探讨其与银屑病皮损和面积严重度指数（PASI）的相关性。结果利用Agile...目的：探讨寻常性银屑病患者皮损及外周血miRNA表达谱系，寻找表达一致的miRNA。方法用基因芯片技术筛选17例银屑病皮损和外周血miRNA，通过验证筛选出差异性miRNA，探讨其与银屑病皮损和面积严重度指数（PASI）的相关性。结果利用Agilent Human miRNA芯片技术,得出银屑病皮损和外周血中相一致的15个miRNA，经过RT-qPCR技术验证，表达一致的miRNA有7个，其中miR-30e-5p、miR-192-5p、miR-17-3p、miR-1227-5p的表达量与银屑病患者PASI评分呈负相关（P＜0.05）；miR-125b-5p、miR-642a-5p、miR-29a-5p与PASI评分无明显相关性（P＞0.05）。结论寻常性银屑病患者皮损组织和血浆中存在着表达一致的miRNA，这些miRNA有望作为评估银屑病患者病情严重程度的生物标记物之一。展开更多
文摘目的:应用寡核苷酸基因芯片技术,研究血瘀证患者差异基因表达谱。方法:16例血瘀证患者经冠状动脉造影诊断后,分为冠心病血瘀证组和非冠心病血瘀证组,每组8例;并选年龄和性别相匹配的8名健康人为健康对照组。抽取静脉血,分离白细胞,抽提RNA,质控芯片(Test3芯片)对样本质量进行检测,然后与Affymetrix U133 Plus 2.0芯片进行杂交,通过扫描和软件分析,比较冠心病血瘀证组、非冠心病血瘀证组与健康对照组的基因表达谱,筛选血瘀证相关差异基因,并进一步进行基因本体(Gene Ontology,GO)和通路分析,使用实时荧光定量逆转录聚合酶链反应法对目标基因进行验证。结果:通过差异基因筛选,与血瘀证相关的差异基因共有48个,其中上调基因26个,下调基因22个;通过GO分析,其中与炎症免疫相关的基因有5个,占10.4%;通路分析结果显示,有意义的10条通路中有5个涉及炎症和免疫反应。经实时荧光定量逆转录聚合酶链反应验证了基因芯片准确可靠。结论:血瘀证基因表达谱研究显示了炎症和免疫相关基因的比例和显著性优势,说明炎症和免疫反应在一定程度上介导了血瘀证的发生发展。
基金Supported by The Basic Research Program of the Korea Science & Engineering Foundation,No.R01-2006-000-10021-0the Korea Health 21 R&D Project,Ministry of Health & Welfare No.A062254
文摘AIM： To investigate alternative or subordinate pathways involved in colorectal tumorigenesis and tumor growth, possibly determining at-risk populations and predicting responses to treatment. METHODS： Using microarray gene-expression analysis, we analyzed patterns of gene expression relative to canonical molecular changes and clinicopathological features in 84 sporadic colorectal cancer patients, standardized by tumor location. Subsets of differentially expressed genes were confirmed by real-time reverse-transcript polymerase chain reaction （RT-PCR）. RESULTS： The largest number of genes identified as being differentially expressed was by tumor location, and the next largest number by lymphovascular or neural invasion of tumor cells and by mismatch repair （NMR） defects. Amongst biological processes, the immune response was significantly implicated in entire molecular changes observed during colorectal tumorigenesis （P 〈 0.001）. Amongst 47 differentially expressed genes, seven （PISD, NIBP, BAI2, STOML1, MRPL21, MRPL16, and MKKS） were newly found to correlate with tumorigenesis and tumor growth. Most location-associated molecular changes had distinct effects on gene expression, but the effects of the latter were sometimes contradictory. CONCLUSION： We show that several differentially expressed genes were associated with canonical molecular changes in sporadic colorectal cancers, possibly constituting alternative or subordinate pathways of tumorigenesis. As tumor location was the dominant factor influencing differential gene expression, location-specific analysis may identify location-associated pathways and enhance the accuracy of class prediction.
文摘目的观察淫羊藿总黄酮(epi medium flavonoids,EF)对皮质酮大鼠肾上腺皮质再生的调控作用。方法采用碘化丙啶染色流式细胞术检测细胞周期分布和细胞凋亡率;用末端转移酶介导的X-dUTP缺口末端标记(TUNEL)法原位显示凋亡细胞;用基因芯片技术检测mRNA表达。结果模型组与正常对照组比较,肾上腺细胞G0/G1期阻滞,凋亡率明显升高(P<0·05);EF干预后G0/G1期比例下降,G2/M期比例显著上升(P<0·01),凋亡率下降(P<0·05)。TUNEL法原位细胞凋亡检测显示正常对照组、模型组、治疗组凋亡阳性细胞数分别为(4·67±1·53)、(70·67±9·29)、(18·67±7·64)个(n=3)。皮质酮主要抑制肾上腺的基因表达,其中7个促进细胞周期的基因如V-ras、V-jun均下调。EF治疗后,肾上腺基因表达以上调为主,其中有6个促进细胞周期基因、1个抗凋亡基因,与肾上腺皮质细胞再生密切相关的上游分子IGF-Ⅱ及其受体IGFR,FGF7及其受体FGFR基因,7个参与类固醇生物合成的基因均上调。结论EF通过促进肾上腺皮质细胞增殖、抑制其细胞凋亡、促进类固醇生物合成,从而促进肾上腺皮质再生是EF在激素撤除过程中发挥保护肾上腺皮质功能的机制。
基金86 3HighTechnologyProject (No # 10 2 10 0 1 0 9)andSpecialFoundationforPh DTrainingProgramofEducationMinistry (No 19990 0
文摘OBJECTIVE: To investigate genes involved in cancer metastasis, mRNA differential display was used to compare the levels of gene expression in two cell sublines derived from human giant cell carcinoma of lung (PG) which had different metastatic potentials. METHODS: Using in vivo tumorigenicity and a spontaneous metastasis assay in nude mice, two sublines (BE1, LH7) from human giant cell carcinoma of lung (PG) with different metastatic potentials were isolated and characterized. mRNA differential display was used to compare the levels of gene expression between them and the obtained results were confirmed by Northern hybridization. RESULTS: One differentially expressed band was nearly identical (99% homology) to Ras-GTPase-Activating protein SH3 domain binding protein (G3BP). G3BP displayed a strong expression in LH7 (non-metastatic in recipient nude mice) and a very weak expression in BE1 (100% metastatic frequency). The same different expression level of G3BP was detected in Northern hybridization with another panel of cell sublines with different metastatic potentials (established in our lab) derived from human prostate carcinoma cell line PC-3M. CONCLUSION: Our results indicate that G3BP was implicated in cancer metastasis because of its differential expressions in the two panels of cell sublines with different metastatic potentials.
文摘目的在整体动物水平研究淫羊藿总黄酮(EF)延缓衰老的效果和分子机制。方法采用果蝇寿命实验,观察EF延长果蝇寿命的效果;采用果蝇全基因组寡核苷酸芯片研究EF延缓衰老的机制。结果空白对照组、V it E组、EF低剂量组及高剂量组平均寿命分别为(34.90±23.04),(35.25±23.41),(41.48±25.00)和(46.23±26.20)d,高低剂量EF均显著延长果蝇的平均寿命(P<0.01或P<0.05),高剂量EF提高平均寿命32.46%。老年果蝇呼吸链相关酶、蛋白分解酶、促凋亡基因上调2倍以上,而自由基清除酶、抗凋亡基因、内分泌免疫相关基因下调2倍以上。EF能下调老年果蝇呼吸链相关酶而上调自由基清除酶基因,下调蛋白分解酶基因,能上调促进细胞周期、抗凋亡、免疫相关基因。结论老年果蝇存在自由基产生过多而清除减少、凋亡加速、蛋白溶解过度、内分泌免疫功能低下的内在机制,EF通过减少自由基的产生,增加自由基的清除,减少蛋白分解,促进细胞增殖,恢复免疫功能、对抗凋亡而发挥延缓衰老的功效。
文摘目的：探讨寻常性银屑病患者皮损及外周血miRNA表达谱系，寻找表达一致的miRNA。方法用基因芯片技术筛选17例银屑病皮损和外周血miRNA，通过验证筛选出差异性miRNA，探讨其与银屑病皮损和面积严重度指数（PASI）的相关性。结果利用Agilent Human miRNA芯片技术,得出银屑病皮损和外周血中相一致的15个miRNA，经过RT-qPCR技术验证，表达一致的miRNA有7个，其中miR-30e-5p、miR-192-5p、miR-17-3p、miR-1227-5p的表达量与银屑病患者PASI评分呈负相关（P＜0.05）；miR-125b-5p、miR-642a-5p、miR-29a-5p与PASI评分无明显相关性（P＞0.05）。结论寻常性银屑病患者皮损组织和血浆中存在着表达一致的miRNA，这些miRNA有望作为评估银屑病患者病情严重程度的生物标记物之一。
