为了探究KAP1.1基因(keratin-associated protein 1-1)潜在的生物学功能,利用分子生物学软件对绵羊KAP1.1基因编码蛋白进行了生物信息学分析。结果表明,该基因共编码182个氨基酸,为一可溶性不稳定蛋白。分子量为18788.45 u,等电点为6.0...为了探究KAP1.1基因(keratin-associated protein 1-1)潜在的生物学功能,利用分子生物学软件对绵羊KAP1.1基因编码蛋白进行了生物信息学分析。结果表明,该基因共编码182个氨基酸,为一可溶性不稳定蛋白。分子量为18788.45 u,等电点为6.03。另预测得知该蛋白含有6个磷酸化位点,无糖基化位点,不含有跨膜区和信号肽,推测其不是一个分泌型蛋白。亚细胞定位绵羊KAP1.1蛋白主要在线粒体和细胞核中发挥生物学功能,二级、三级结构显示,无规则卷曲是构成该蛋白的主要结构元件。物种间同源性分析显示,绵羊KAP1.1蛋白氨基酸组成与牛的相似度最高(85.9%)。展开更多
Dear Editor,Epstein-Barr virus(EBV,also termed human herpesvirus-4)was the first identified human tumor virus.Since its discovery in 1964,studies have shown that EBV infects over 90%of all people by the time they are ...Dear Editor,Epstein-Barr virus(EBV,also termed human herpesvirus-4)was the first identified human tumor virus.Since its discovery in 1964,studies have shown that EBV infects over 90%of all people by the time they are adults(Williams and Crawford 2006).EBV infection can result in mucocutaneous and systemic diseases,ranging from selflimited illnesses to aggressive malignancies,including B cell Hodgkin lymphoma and nasopharyngeal carcinoma.In vitro,EBV transforms resting B cells into proliferating blast cells(Pope et al.1968).展开更多
Dear Editor,Protein-protein interactions（PPIs）often play important roles in biological processes（Zhang et al.,2016）.The split Renilla luciferase complementation assay（SRLCA）is one of the methods in studying PPIs...Dear Editor,Protein-protein interactions（PPIs）often play important roles in biological processes（Zhang et al.,2016）.The split Renilla luciferase complementation assay（SRLCA）is one of the methods in studying PPIs.SRLCA is based on the complementation of the N-terminal domains of Renilla luciferase（LN）and C-terminal domains of Renilla luciferase （LC） non-functional halves of Renilla luciferase fused to possibly interacting proteins and emit luminescence （Deng et al., 2011; Jiang et al., 2010） （Supplementary Figure S1A）.展开更多
Synthesis of macromolecular systems with precise structural and functional control constitutes a fundamental challenge for materials science and engineering. Development of the ability to construct complex bio-macromo...Synthesis of macromolecular systems with precise structural and functional control constitutes a fundamental challenge for materials science and engineering. Development of the ability to construct complex bio-macromolecular architectures provides a solution to this challenge. The past few years have witnessed the emergence of a new category of peptide-protein chemistry which can covalently stitch together protein]peptide molecules with high specificity under mild physiological conditions. It has thus inspired the concept of genetically encoded click chemistry （GECC）. As a prototype of GECC, SpyTag/ SpyCatcher chemistry has enabled the precise synthesis ofmacromolecules both in vitro and in vivo, exerting precise control over the fundamental properties of these macromolecules including length, sequence, stereochemistry and topology and leading to the creation of diverse biomaterials for a variety of applications. We thus anticipate a potential toolbox of GECC comprising multiple mutually orthogonal, covalent-bond forming peptide-protein reactive pairs with diverse features, which shall bridge synthetic biology and materials science and open up enormous opportunities for biomaterialsin the future.展开更多
文摘为了探究KAP1.1基因(keratin-associated protein 1-1)潜在的生物学功能,利用分子生物学软件对绵羊KAP1.1基因编码蛋白进行了生物信息学分析。结果表明,该基因共编码182个氨基酸,为一可溶性不稳定蛋白。分子量为18788.45 u,等电点为6.03。另预测得知该蛋白含有6个磷酸化位点,无糖基化位点,不含有跨膜区和信号肽,推测其不是一个分泌型蛋白。亚细胞定位绵羊KAP1.1蛋白主要在线粒体和细胞核中发挥生物学功能,二级、三级结构显示,无规则卷曲是构成该蛋白的主要结构元件。物种间同源性分析显示,绵羊KAP1.1蛋白氨基酸组成与牛的相似度最高(85.9%)。
基金supported by the National Natural Science Foundation of China (Grant Numbers: 81402542 and 81772166)the scholarship of Pujiang Talents in Shanghai to Fang Wei (Grant Number: 14PJ1405600)
文摘Dear Editor,Epstein-Barr virus(EBV,also termed human herpesvirus-4)was the first identified human tumor virus.Since its discovery in 1964,studies have shown that EBV infects over 90%of all people by the time they are adults(Williams and Crawford 2006).EBV infection can result in mucocutaneous and systemic diseases,ranging from selflimited illnesses to aggressive malignancies,including B cell Hodgkin lymphoma and nasopharyngeal carcinoma.In vitro,EBV transforms resting B cells into proliferating blast cells(Pope et al.1968).
基金supported by the Initiative Research Program of Wuhan University(No.410100020)the advanced talent independent research program of Wuhan University(No.410100011)the National Natural Science Foundation of China(No.210700228)
文摘Dear Editor,Protein-protein interactions（PPIs）often play important roles in biological processes（Zhang et al.,2016）.The split Renilla luciferase complementation assay（SRLCA）is one of the methods in studying PPIs.SRLCA is based on the complementation of the N-terminal domains of Renilla luciferase（LN）and C-terminal domains of Renilla luciferase （LC） non-functional halves of Renilla luciferase fused to possibly interacting proteins and emit luminescence （Deng et al., 2011; Jiang et al., 2010） （Supplementary Figure S1A）.
基金financial supports from the Research Grants Council of Hong Kong SAR Government to F. Sun (RGC-ECS Nos. #26103915 and Ao E/M-09/12)the 863 Program (No. 2015AA020941)+2 种基金the National Natural Science Foundation of China (Nos. 21474003, 91427304)"1000 Plan (Youth)"the Department of Chemical and Biological Engineering, HKUST for the faculty start-up fund
文摘Synthesis of macromolecular systems with precise structural and functional control constitutes a fundamental challenge for materials science and engineering. Development of the ability to construct complex bio-macromolecular architectures provides a solution to this challenge. The past few years have witnessed the emergence of a new category of peptide-protein chemistry which can covalently stitch together protein]peptide molecules with high specificity under mild physiological conditions. It has thus inspired the concept of genetically encoded click chemistry （GECC）. As a prototype of GECC, SpyTag/ SpyCatcher chemistry has enabled the precise synthesis ofmacromolecules both in vitro and in vivo, exerting precise control over the fundamental properties of these macromolecules including length, sequence, stereochemistry and topology and leading to the creation of diverse biomaterials for a variety of applications. We thus anticipate a potential toolbox of GECC comprising multiple mutually orthogonal, covalent-bond forming peptide-protein reactive pairs with diverse features, which shall bridge synthetic biology and materials science and open up enormous opportunities for biomaterialsin the future.
