摘要
Dear Editor,Protein-protein interactions（PPIs）often play important roles in biological processes（Zhang et al.,2016）.The split Renilla luciferase complementation assay（SRLCA）is one of the methods in studying PPIs.SRLCA is based on the complementation of the N-terminal domains of Renilla luciferase（LN）and C-terminal domains of Renilla luciferase （LC） non-functional halves of Renilla luciferase fused to possibly interacting proteins and emit luminescence （Deng et al., 2011; Jiang et al., 2010） （Supplementary Figure S1A）.
Dear Editor,Protein-protein interactions(PPIs)often play important roles in biological processes(Zhang et al.,2016).The split Renilla luciferase complementation assay(SRLCA)is one of the methods in studying PPIs.SRLCA is based on the complementation of the N-terminal domains of Renilla luciferase(LN)and C-terminal domains
作者
Chao Wang
Jingchao Wang
Wei Guo
Cong Long
Huan Wang
Xiaoping Sun
Chao Wang Jingchao Wang Wei Guo Cong Long Huan Wang Xiaoping Sun(Stat Key Laboratory of Virology, Department of Pathogen Biology, School of Basic Medical Sciences, Wuhan University;Department of Pathology and Physiology, School of Basic Medical Sciences, Wuhan University;State Key laboratory of Virology, Wuhan University)
基金
supported by the Initiative Research Program of Wuhan University(No.410100020)
the advanced talent independent research program of Wuhan University(No.410100011)
the National Natural Science Foundation of China(No.210700228)
