目的了解2021年桂林市腹泻病源沙门菌菌株表型特征及分子分型特征,建立相关数据库,为桂林市控制沙门菌引起的腹泻病传播和疫情溯源提供实验室有效数据。方法通过对菌株进行血清学鉴定、脉冲场凝胶电泳(pulsed field gel electrophoresis...目的了解2021年桂林市腹泻病源沙门菌菌株表型特征及分子分型特征,建立相关数据库,为桂林市控制沙门菌引起的腹泻病传播和疫情溯源提供实验室有效数据。方法通过对菌株进行血清学鉴定、脉冲场凝胶电泳(pulsed field gel electrophoresis,PFGE)试验和药物敏感试验3类项目检测,采用国家致病菌识别网信息化平台系统对其结果进行关联研究,确定菌株间的亲缘性。结果2021年来自桂林市3家医院患者的42株沙门菌经生化鉴定及血清凝集试验,得到了11种血清型,其中优势菌有鼠伤寒沙门菌(17株)、伦敦沙门菌(7株)和肠炎沙门菌(5株);药物敏感试验结果显示(16种抗生素):氨苄西林(ampicillin,AMP)(61.90%)耐药率最高,其次是复方新诺明(sulfamethoxazole,SXT)(59.52%)。多粘菌素E(colistin E,ECT)、美罗培南(meropenem,MEM)、厄他培南(ertapenem,ETP)、替加环素(tigecycline,TIG)、阿米卡星(amikacin,AMI)5种为敏感药物,敏感率均为100.00%。多重耐药菌株有28株(66.67%),最多见是4耐药物,耐药谱为氯霉素(chloramphenicol,CHL)-复方新诺明(SXT)-四环素(TET)-氨苄西林(AMP)(7株);耐药种类最高的是8耐,耐药谱为氯霉素(CHL)-复方新诺明(SXT)-头孢噻肟(cefotaxime,CTX)-四环素(TET)-环丙沙星(ciprofloxacin,CIP)-萘啶酸(nalidixic acid,NAL)-氨苄西林(AMP)-氨苄西林/舒巴坦(ampi⁃cillin/sulbactam,AMS);42株沙门菌经过聚类分析可分为38种带型图谱,显示出较大的遗传多样性。结论2021年桂林市腹泻病源沙门菌的血清型主要以鼠伤寒沙门菌、伦敦沙门菌和肠炎沙门菌为主,耐药状况和多重耐药率不容乐观,PFGE带型图谱呈遗传多样性,血清型、耐药谱与PFGE分型无明显关联。展开更多
[ Objective] This paper aimed to understand drug resistance of Escherichia coli isolated from pets in Guangzhou. I Method ] Broth microdilution method was used to determine the minimal inhibitory concentration （MIC）...[ Objective] This paper aimed to understand drug resistance of Escherichia coli isolated from pets in Guangzhou. I Method ] Broth microdilution method was used to determine the minimal inhibitory concentration （MIC） of ceftiofur, enrofloxacin and other 11 kinds of antibiotics for antimicrobial agent susceptibility testing of 120 strains of clinical E. coli and the experimental data were processed by WHONET 5.4 software. Based on the results of drug resistance pattern analysis, it helped to analyze and study the resistance mechanism. EResultl Clinical E. coli isolated from pets showed a different drug resistance to the 13 kinds of veterinary clinical antibiotics, and the different sources of E. coli also showed a different drug resistance pattern.[Cenclmion] The study provided a theoretical basis for the clinical use of drugs and druq resistance surveillance.展开更多
文摘目的了解2021年桂林市腹泻病源沙门菌菌株表型特征及分子分型特征,建立相关数据库,为桂林市控制沙门菌引起的腹泻病传播和疫情溯源提供实验室有效数据。方法通过对菌株进行血清学鉴定、脉冲场凝胶电泳(pulsed field gel electrophoresis,PFGE)试验和药物敏感试验3类项目检测,采用国家致病菌识别网信息化平台系统对其结果进行关联研究,确定菌株间的亲缘性。结果2021年来自桂林市3家医院患者的42株沙门菌经生化鉴定及血清凝集试验,得到了11种血清型,其中优势菌有鼠伤寒沙门菌(17株)、伦敦沙门菌(7株)和肠炎沙门菌(5株);药物敏感试验结果显示(16种抗生素):氨苄西林(ampicillin,AMP)(61.90%)耐药率最高,其次是复方新诺明(sulfamethoxazole,SXT)(59.52%)。多粘菌素E(colistin E,ECT)、美罗培南(meropenem,MEM)、厄他培南(ertapenem,ETP)、替加环素(tigecycline,TIG)、阿米卡星(amikacin,AMI)5种为敏感药物,敏感率均为100.00%。多重耐药菌株有28株(66.67%),最多见是4耐药物,耐药谱为氯霉素(chloramphenicol,CHL)-复方新诺明(SXT)-四环素(TET)-氨苄西林(AMP)(7株);耐药种类最高的是8耐,耐药谱为氯霉素(CHL)-复方新诺明(SXT)-头孢噻肟(cefotaxime,CTX)-四环素(TET)-环丙沙星(ciprofloxacin,CIP)-萘啶酸(nalidixic acid,NAL)-氨苄西林(AMP)-氨苄西林/舒巴坦(ampi⁃cillin/sulbactam,AMS);42株沙门菌经过聚类分析可分为38种带型图谱,显示出较大的遗传多样性。结论2021年桂林市腹泻病源沙门菌的血清型主要以鼠伤寒沙门菌、伦敦沙门菌和肠炎沙门菌为主,耐药状况和多重耐药率不容乐观,PFGE带型图谱呈遗传多样性,血清型、耐药谱与PFGE分型无明显关联。
基金supported by the Special Fund Project for the Institute Science and Technology Development and Demostration Extention of Henan Provincial Academy of Argricultural Sciences(Research of Zoonotic Small Animal Diseases)the Project for the 2012 Annual"University Students'Innovation Training Program",Southwest University for Nationalities(201210656017)the Special Fund Project for the Central University Basic Scientific Research Fund,Southwest University for Nationalities(11NZYTD02)
文摘[ Objective] This paper aimed to understand drug resistance of Escherichia coli isolated from pets in Guangzhou. I Method ] Broth microdilution method was used to determine the minimal inhibitory concentration （MIC） of ceftiofur, enrofloxacin and other 11 kinds of antibiotics for antimicrobial agent susceptibility testing of 120 strains of clinical E. coli and the experimental data were processed by WHONET 5.4 software. Based on the results of drug resistance pattern analysis, it helped to analyze and study the resistance mechanism. EResultl Clinical E. coli isolated from pets showed a different drug resistance to the 13 kinds of veterinary clinical antibiotics, and the different sources of E. coli also showed a different drug resistance pattern.[Cenclmion] The study provided a theoretical basis for the clinical use of drugs and druq resistance surveillance.
