New fluorescent silica nanoparticles for sensors simultaneous determination of double targets were synthesized via a reverse microemulsion method based on the fluorescence resonance energy transfer(FRET).Nanoparticle ...New fluorescent silica nanoparticles for sensors simultaneous determination of double targets were synthesized via a reverse microemulsion method based on the fluorescence resonance energy transfer(FRET).Nanoparticle A was doped with a dye complex which consisted of two dye molecules,fluorescein and tetramethylrhodamine.These dye molecules were first linked with avidin and biotin,respectively.They then formed a stable dye complex through avidin-biotin bridge within the distance of FRET.Nanoparticle B was doped with fluorescein only.As a result of this combination,the nanoparticles emit double wavelengths when a single excitation wavelength is used.The results show that the fluorescent nanoparticles are promising fluorescent labeling reagents for the sensitive detection of double targets.展开更多
采用蒙特卡罗方法建立地海交界分区域复合粗糙面模型,陆地区域采用高斯谱模拟,近海海面区域采用基于变浅系数的北海海浪工程(joint North Sea wave project,JONSWAP)谱模拟;提出了基于分形理论的边界赋形方法,并运用多种反正切权函数处...采用蒙特卡罗方法建立地海交界分区域复合粗糙面模型,陆地区域采用高斯谱模拟,近海海面区域采用基于变浅系数的北海海浪工程(joint North Sea wave project,JONSWAP)谱模拟;提出了基于分形理论的边界赋形方法,并运用多种反正切权函数处理实现了线型、月牙型、峡谷型和分形型边界的地海交界环境;基于矩量法和基尔霍夫近似的混合算法研究了地海交界环境与其上方双目标的复合电磁散射特性,对比了双目标分别位于两种典型环境和地海交界环境上方时的复合散射系数曲线,研究了复合散射系数随入射角、陆地区域参数、海面参数、边界形状、双目标参数及双目标类型的变化情况,并做了详细分析与讨论。结果表明该混合算法的高效性和准确性,数值仿真结果对地海交界环境上方目标的探测、遥感及成像具有借鉴意义。展开更多
文摘New fluorescent silica nanoparticles for sensors simultaneous determination of double targets were synthesized via a reverse microemulsion method based on the fluorescence resonance energy transfer(FRET).Nanoparticle A was doped with a dye complex which consisted of two dye molecules,fluorescein and tetramethylrhodamine.These dye molecules were first linked with avidin and biotin,respectively.They then formed a stable dye complex through avidin-biotin bridge within the distance of FRET.Nanoparticle B was doped with fluorescein only.As a result of this combination,the nanoparticles emit double wavelengths when a single excitation wavelength is used.The results show that the fluorescent nanoparticles are promising fluorescent labeling reagents for the sensitive detection of double targets.
文摘采用蒙特卡罗方法建立地海交界分区域复合粗糙面模型,陆地区域采用高斯谱模拟,近海海面区域采用基于变浅系数的北海海浪工程(joint North Sea wave project,JONSWAP)谱模拟;提出了基于分形理论的边界赋形方法,并运用多种反正切权函数处理实现了线型、月牙型、峡谷型和分形型边界的地海交界环境;基于矩量法和基尔霍夫近似的混合算法研究了地海交界环境与其上方双目标的复合电磁散射特性,对比了双目标分别位于两种典型环境和地海交界环境上方时的复合散射系数曲线,研究了复合散射系数随入射角、陆地区域参数、海面参数、边界形状、双目标参数及双目标类型的变化情况,并做了详细分析与讨论。结果表明该混合算法的高效性和准确性,数值仿真结果对地海交界环境上方目标的探测、遥感及成像具有借鉴意义。
