热激蛋白70(HSP70,heat shock protein 70)广泛参与胁迫环境的响应,在诱发人体肿瘤细胞凋亡,增强肿瘤的免疫原性中起着重要作用。然而,植物HSP70的生理功能研究起步较晚,最近的研究发现植物HSP70在细胞内主要参与新生肽的折叠与成熟、...热激蛋白70(HSP70,heat shock protein 70)广泛参与胁迫环境的响应,在诱发人体肿瘤细胞凋亡,增强肿瘤的免疫原性中起着重要作用。然而,植物HSP70的生理功能研究起步较晚,最近的研究发现植物HSP70在细胞内主要参与新生肽的折叠与成熟、损伤蛋白的降解和蛋白运输;植物HSP70在非生物胁迫环境的应答、抗病性及植物发育中起着重要作用。本文从分子生物学角度,系统综述了植物HSP70分子作用机理研究的进展,以及在提高植物抗逆性方面的作用,以期为基因工程方法改良作物抗性提供参考。展开更多
热激蛋白90(HSP90,heat shock protein 90)广泛介导了胁迫信号的传递,在控制人体细胞正常生长和促进肿瘤细胞发育中起着重要作用。目前,HSP90已成为细胞免疫、信号转导以及抗肿瘤研究的前沿课题。但植物HSP90的生理功能研究起步较晚,最...热激蛋白90(HSP90,heat shock protein 90)广泛介导了胁迫信号的传递,在控制人体细胞正常生长和促进肿瘤细胞发育中起着重要作用。目前,HSP90已成为细胞免疫、信号转导以及抗肿瘤研究的前沿课题。但植物HSP90的生理功能研究起步较晚,最近的研究发现HSP90在植物发育、胁迫环境的应答以及抗病性中起着重要作用。本文从分子生物学角度,系统综述了植物HSP90分子作用机理研究的最新进展,以及在改良植物抗性上的应用,以期为通过基因工程方法改良作物抗性提供参考。展开更多
In this study, we identified a defense-related major latex protein （MLP） from upland cotton （designated GhMLP28） and investigated its functional mechanism. GhMLP28 transcripts were ubiquitously present in cotton p...In this study, we identified a defense-related major latex protein （MLP） from upland cotton （designated GhMLP28） and investigated its functional mechanism. GhMLP28 transcripts were ubiquitously present in cotton plants, with higher accumulation in the root. Expression of the GhMLP28 gene was induced by Verticillium dahliae inoculation and was responsive to defense signaling molecules, including ethylene, jas- monic acid, and salicylic acid. Knockdown of GhMLP28 expression by virus-induced gene silencing re- sulted in increased susceptibility of cotton plants to V. dahliae infection, while ectopic overexpression of GhMLP28 in tobacco improved the disease tolerance of the transgenic plants. Further analysis revealed that GhMLP28 interacted with cotton ethylene response factor 6 （GhERF6） and facilitated the binding of GhERF6 to GCC-box element. Transient expression assay demonstrated that GhMLP28 enhanced the tran- scription factor activity of GhERF6, which led to the augmented expression of some GCC-box genes. GhMLP28 proteins were located in both the nucleus and cytoplasm and their nuclear distribution was dependent on the presence of GhERF6. Collectively, these results demonstrate that GhMLP28 acts as a positive regulator of GhERF6, and synergetic actions of the two proteins may contribute substantially to protection against V. dahliae infection in cotton plants.展开更多
Due to the meteorological factors, Henan Province had a large-scale outbreak of corn stalk rot in 2014, and the incidence status of 25 varieties was investigated. According to the relevant resistance identification st...Due to the meteorological factors, Henan Province had a large-scale outbreak of corn stalk rot in 2014, and the incidence status of 25 varieties was investigated. According to the relevant resistance identification standards, Xundan.509 was classified as resistance variety; Yudan606, Jinsai38 and Xundan3136 were classified as moderate resistance ones, while the other 21 varieties were highly susceptible varieties. Using the yield loss rate of 5% as the critical value of disease tolerance standard, XundanS09, Yudan606, Jinsai38, Xundan3136, Huaiyu5288, QiaoyuS, XY046, Zhengyu10, Lile66, WeikeT02 and Xundan29 were classified into tolerance varieties of corn stalk rot. The concept between disease resistance and disease tolerance was distinguished. The diseased plant rate was used as the classification basis of disease resistance and the yield loss rate was used as the evaluation standard of disease tolerance. The relationship between both was showed by a Venn diagram.展开更多
利用12个小麦品质、抗病和抗逆等相关的KASP标记,对河北省育成的部分旱碱麦和农家种(54份)进行KASP标记检测。结果表明,在品质方面,有15份材料含有Glu-D1基因的高分子量麦谷蛋白亚基5+10,有32份材料是Glu-A1基因的Ax1 or Ax2*强筋单倍...利用12个小麦品质、抗病和抗逆等相关的KASP标记,对河北省育成的部分旱碱麦和农家种(54份)进行KASP标记检测。结果表明,在品质方面,有15份材料含有Glu-D1基因的高分子量麦谷蛋白亚基5+10,有32份材料是Glu-A1基因的Ax1 or Ax2*强筋单倍型。在抗病性方面,有25份含有抗叶锈病基因Lr34,有27份含有抗赤霉病基因Fhb1。在抗穗发芽方面,有24份材料含TaSdr-B1基因的TaSdr-B1a抗穗发芽单倍型;有47份材料含有TaPHS1基因的Rio Blanco type抗穗发芽单倍型;有9份材料含有TaMoc-A1基因的Hap-H抗穗发芽单倍型。在抗旱性方面,有44份材料含有COMT-3B基因的3Ba抗旱单倍型;有12份材料含有Dreb-B1基因的TaDREB-B1a抗旱单倍型;有49份材料含有TaSST-4A基因的A2a抗旱单倍型。展开更多
Stalk rot outbroke in Henan Province in 2014 on maize,and the disease conditions of 25 varieties were investigated.According to national maize variety disease resistance identification standards,Jundan 509 was a resis...Stalk rot outbroke in Henan Province in 2014 on maize,and the disease conditions of 25 varieties were investigated.According to national maize variety disease resistance identification standards,Jundan 509 was a resistant variety; Yudan 606,Jinsai 38 and Jundan 3136 were moderately-resistant varieties; and other 21 varieties were all highly susceptible.Based on the yield loss rate of 5% determined as the critical value for division of disease tolerance,disease-tolerant varieties included Jundan 509,Yudan 606,Jinsai 38,Jundan 3136,Huaiyu 5288,Qiaoyu 8,XY046,Zhengyu 10,lile 66,weike 702 and Jundan 29.Varietal disease resistance and tolerance were mainly divided according to incidence and yield loss rate,respectively,and the correlation between them was showed by a Venn diagram.With the male parent Jun M9 of Jundan 509 as a backbone material,multiple maize combinations resistant to stalk rot were formed,and Yongyou 618 among them was approved by Henan Provincial Crop Variety Approval Committee.展开更多
猪繁殖与呼吸综合征(porcine reproductive and respiratory syndrome,PRRS)是近期危害养猪业最重要的疾病之一。培育抗病性品种是该病综合防治的一个有效方法。文章围绕该病抗病育种候选基因研究的主要进展进行综述,内容涉及病毒特异...猪繁殖与呼吸综合征(porcine reproductive and respiratory syndrome,PRRS)是近期危害养猪业最重要的疾病之一。培育抗病性品种是该病综合防治的一个有效方法。文章围绕该病抗病育种候选基因研究的主要进展进行综述,内容涉及病毒特异性受体基因、先天性免疫相关基因、抗原递呈信号通路相关基因、胞内miRNA等,并重点就今后PRRSV疾病耐受性和提高适应性免疫应答能力的研究进行展望。展开更多
文摘热激蛋白70(HSP70,heat shock protein 70)广泛参与胁迫环境的响应,在诱发人体肿瘤细胞凋亡,增强肿瘤的免疫原性中起着重要作用。然而,植物HSP70的生理功能研究起步较晚,最近的研究发现植物HSP70在细胞内主要参与新生肽的折叠与成熟、损伤蛋白的降解和蛋白运输;植物HSP70在非生物胁迫环境的应答、抗病性及植物发育中起着重要作用。本文从分子生物学角度,系统综述了植物HSP70分子作用机理研究的进展,以及在提高植物抗逆性方面的作用,以期为基因工程方法改良作物抗性提供参考。
文摘热激蛋白90(HSP90,heat shock protein 90)广泛介导了胁迫信号的传递,在控制人体细胞正常生长和促进肿瘤细胞发育中起着重要作用。目前,HSP90已成为细胞免疫、信号转导以及抗肿瘤研究的前沿课题。但植物HSP90的生理功能研究起步较晚,最近的研究发现HSP90在植物发育、胁迫环境的应答以及抗病性中起着重要作用。本文从分子生物学角度,系统综述了植物HSP90分子作用机理研究的最新进展,以及在改良植物抗性上的应用,以期为通过基因工程方法改良作物抗性提供参考。
文摘In this study, we identified a defense-related major latex protein （MLP） from upland cotton （designated GhMLP28） and investigated its functional mechanism. GhMLP28 transcripts were ubiquitously present in cotton plants, with higher accumulation in the root. Expression of the GhMLP28 gene was induced by Verticillium dahliae inoculation and was responsive to defense signaling molecules, including ethylene, jas- monic acid, and salicylic acid. Knockdown of GhMLP28 expression by virus-induced gene silencing re- sulted in increased susceptibility of cotton plants to V. dahliae infection, while ectopic overexpression of GhMLP28 in tobacco improved the disease tolerance of the transgenic plants. Further analysis revealed that GhMLP28 interacted with cotton ethylene response factor 6 （GhERF6） and facilitated the binding of GhERF6 to GCC-box element. Transient expression assay demonstrated that GhMLP28 enhanced the tran- scription factor activity of GhERF6, which led to the augmented expression of some GCC-box genes. GhMLP28 proteins were located in both the nucleus and cytoplasm and their nuclear distribution was dependent on the presence of GhERF6. Collectively, these results demonstrate that GhMLP28 acts as a positive regulator of GhERF6, and synergetic actions of the two proteins may contribute substantially to protection against V. dahliae infection in cotton plants.
基金Supported by Major Science and Technology Project of Henan Province"Gene Mining of Important Agronomic Traits in Major Crops and Breeding of New Corn Varieties"(161100110500-0104)
文摘Due to the meteorological factors, Henan Province had a large-scale outbreak of corn stalk rot in 2014, and the incidence status of 25 varieties was investigated. According to the relevant resistance identification standards, Xundan.509 was classified as resistance variety; Yudan606, Jinsai38 and Xundan3136 were classified as moderate resistance ones, while the other 21 varieties were highly susceptible varieties. Using the yield loss rate of 5% as the critical value of disease tolerance standard, XundanS09, Yudan606, Jinsai38, Xundan3136, Huaiyu5288, QiaoyuS, XY046, Zhengyu10, Lile66, WeikeT02 and Xundan29 were classified into tolerance varieties of corn stalk rot. The concept between disease resistance and disease tolerance was distinguished. The diseased plant rate was used as the classification basis of disease resistance and the yield loss rate was used as the evaluation standard of disease tolerance. The relationship between both was showed by a Venn diagram.
文摘利用12个小麦品质、抗病和抗逆等相关的KASP标记,对河北省育成的部分旱碱麦和农家种(54份)进行KASP标记检测。结果表明,在品质方面,有15份材料含有Glu-D1基因的高分子量麦谷蛋白亚基5+10,有32份材料是Glu-A1基因的Ax1 or Ax2*强筋单倍型。在抗病性方面,有25份含有抗叶锈病基因Lr34,有27份含有抗赤霉病基因Fhb1。在抗穗发芽方面,有24份材料含TaSdr-B1基因的TaSdr-B1a抗穗发芽单倍型;有47份材料含有TaPHS1基因的Rio Blanco type抗穗发芽单倍型;有9份材料含有TaMoc-A1基因的Hap-H抗穗发芽单倍型。在抗旱性方面,有44份材料含有COMT-3B基因的3Ba抗旱单倍型;有12份材料含有Dreb-B1基因的TaDREB-B1a抗旱单倍型;有49份材料含有TaSST-4A基因的A2a抗旱单倍型。
基金Supported by Open Research Fund of Henan Provincial Key Laboratory of Agrometeorological Ensuring and Applied Technique,CMA(AMF201703)Henan Science and Technology Major Project(161100110500-0104)
文摘Stalk rot outbroke in Henan Province in 2014 on maize,and the disease conditions of 25 varieties were investigated.According to national maize variety disease resistance identification standards,Jundan 509 was a resistant variety; Yudan 606,Jinsai 38 and Jundan 3136 were moderately-resistant varieties; and other 21 varieties were all highly susceptible.Based on the yield loss rate of 5% determined as the critical value for division of disease tolerance,disease-tolerant varieties included Jundan 509,Yudan 606,Jinsai 38,Jundan 3136,Huaiyu 5288,Qiaoyu 8,XY046,Zhengyu 10,lile 66,weike 702 and Jundan 29.Varietal disease resistance and tolerance were mainly divided according to incidence and yield loss rate,respectively,and the correlation between them was showed by a Venn diagram.With the male parent Jun M9 of Jundan 509 as a backbone material,multiple maize combinations resistant to stalk rot were formed,and Yongyou 618 among them was approved by Henan Provincial Crop Variety Approval Committee.
文摘猪繁殖与呼吸综合征(porcine reproductive and respiratory syndrome,PRRS)是近期危害养猪业最重要的疾病之一。培育抗病性品种是该病综合防治的一个有效方法。文章围绕该病抗病育种候选基因研究的主要进展进行综述,内容涉及病毒特异性受体基因、先天性免疫相关基因、抗原递呈信号通路相关基因、胞内miRNA等,并重点就今后PRRSV疾病耐受性和提高适应性免疫应答能力的研究进行展望。
