The changes of protein secondary structures in the extracellular polymeric substances(EPS) extracted from activated sludge by four different methods were studied by analyzing the amide I region(1700–1600 cm-1) of the...The changes of protein secondary structures in the extracellular polymeric substances(EPS) extracted from activated sludge by four different methods were studied by analyzing the amide I region(1700–1600 cm-1) of the Fourier transform infrared spectra and model protein test. The results showed the molecular weight distribution of organic matter extracted by centrifugation, heating and cation exchange resin(CER) was similar, while the EPS extracted by centrifugation(Control) and CER had similar fluorescent organic matter. The protein secondary structures of extracted EPS by the four methods were different. The similarities of protein secondary structures between the EPS extracted by CER with the Control were the highest among the four extracted EPS. Although the EPS yield extracted by formaldehyde + NaOH method were the highest, its protein secondary structures had the lowest similarity with those extracted by the Control. Additionally, the effects of centrifugation and CER extraction on the secondary structures of bovine serum albumin were also lower than that of other extraction processes. CER enables the second maximum extraction of EPS and maximum retention of the original secondary structure of proteins.展开更多
[目的]研究不同提取方法对佛手多糖理化性质及乙醇脱氢酶活性的影响,为改进佛手多糖生产方式和佛手多糖解酒研究提供依据。[方法]分别采用传统水提法、超声辅助法、微波辅助法、超声-微波协同提取法和复合酶法提取佛手多糖,探讨5种提取...[目的]研究不同提取方法对佛手多糖理化性质及乙醇脱氢酶活性的影响,为改进佛手多糖生产方式和佛手多糖解酒研究提供依据。[方法]分别采用传统水提法、超声辅助法、微波辅助法、超声-微波协同提取法和复合酶法提取佛手多糖,探讨5种提取方法对多糖得率、糖醛酸含量、分子量、单糖组成、红外光谱和乙醇脱氢酶活性的影响。[结果]水提法和超声-微波协同提取多糖得率最高,分别为(4.26±0.21)%和(4.15±0.14)%。不同方法提取的佛手多糖分子量大小存在差异,其中复合酶法提取多糖平均分子量为617.85 k D,约是水提多糖分子量的4倍。5种多糖均呈现出典型的吡喃型多糖和α型糖苷键吸收,单糖组成均以阿拉伯糖、半乳糖、葡萄糖为主。超声-微波协同提取多糖对乙醇脱氢酶激活能力最强,可达(46.58±1.76)%,而传统水提多糖抑制乙醇脱氢酶活性。[结论]超声-微波协同提取佛手多糖得率高且所需时间短,对乙醇脱氢酶激活能力强,推测乙醇脱氢酶激活能力与多糖分子量大小、半乳糖醛酸连接方式有关。展开更多
基金supported by the Major Science and Technology Program for Water Pollution Control and Treatment of China (Nos. 2017ZX07106003-002 and 2017ZX07102004-002)
文摘The changes of protein secondary structures in the extracellular polymeric substances(EPS) extracted from activated sludge by four different methods were studied by analyzing the amide I region(1700–1600 cm-1) of the Fourier transform infrared spectra and model protein test. The results showed the molecular weight distribution of organic matter extracted by centrifugation, heating and cation exchange resin(CER) was similar, while the EPS extracted by centrifugation(Control) and CER had similar fluorescent organic matter. The protein secondary structures of extracted EPS by the four methods were different. The similarities of protein secondary structures between the EPS extracted by CER with the Control were the highest among the four extracted EPS. Although the EPS yield extracted by formaldehyde + NaOH method were the highest, its protein secondary structures had the lowest similarity with those extracted by the Control. Additionally, the effects of centrifugation and CER extraction on the secondary structures of bovine serum albumin were also lower than that of other extraction processes. CER enables the second maximum extraction of EPS and maximum retention of the original secondary structure of proteins.
文摘[目的]研究不同提取方法对佛手多糖理化性质及乙醇脱氢酶活性的影响,为改进佛手多糖生产方式和佛手多糖解酒研究提供依据。[方法]分别采用传统水提法、超声辅助法、微波辅助法、超声-微波协同提取法和复合酶法提取佛手多糖,探讨5种提取方法对多糖得率、糖醛酸含量、分子量、单糖组成、红外光谱和乙醇脱氢酶活性的影响。[结果]水提法和超声-微波协同提取多糖得率最高,分别为(4.26±0.21)%和(4.15±0.14)%。不同方法提取的佛手多糖分子量大小存在差异,其中复合酶法提取多糖平均分子量为617.85 k D,约是水提多糖分子量的4倍。5种多糖均呈现出典型的吡喃型多糖和α型糖苷键吸收,单糖组成均以阿拉伯糖、半乳糖、葡萄糖为主。超声-微波协同提取多糖对乙醇脱氢酶激活能力最强,可达(46.58±1.76)%,而传统水提多糖抑制乙醇脱氢酶活性。[结论]超声-微波协同提取佛手多糖得率高且所需时间短,对乙醇脱氢酶激活能力强,推测乙醇脱氢酶激活能力与多糖分子量大小、半乳糖醛酸连接方式有关。
