A new bibenzyl derivative, 3,4-dihydroxy-4,5-dimethoxy bibenzyl, was isolated from a orchid Dendrobium moniliforme. The structure elucidation and 1H,13C NMR assignments were achieved by spectroscopic method.
A new bibenzyl glycoside has been isolated from the stems of Dendrobium moniliforme (L.) Sw. (Orchidaceae). Its structure has been identified on the basis of spectroscopic and chemical methods.
[ Objective] The study aimed to investigate the effects of silicon on physiology and biochemistry of Dendrobium moniliforme plantlets under low tempera- ture stress. [ Method ] By using Dendrobium monilforme as the ex...[ Objective] The study aimed to investigate the effects of silicon on physiology and biochemistry of Dendrobium moniliforme plantlets under low tempera- ture stress. [ Method ] By using Dendrobium monilforme as the experimental material, different concentrations of Na2SiO3 （0, O. 2, 0.4, 0.6, 0.8, 1.0, 5.0 and 10.0 mmol/L） was added to the basic medium[ 1/2MS ＋ 6-BA （0.1mg/L） ＋ NAA （ 1 mg/L） ＋ agar （7.2 g/L） ＋ sucrose （30 g/L） ] for tissue culture; af- ter hardening and transplanting, Dendrobium moniliforme plantlets were treated under low temperature stress at 4 ~C for 0, 24 and 48 h, in order to investigate the physiological response of Dendrobium ranniliforme leaves to different concentrations of Na2SiO3. [ Result] Under low temperature stress at 4℃, Dendrob/um mon//i- fortns leaves have certain osmotic regulation ability, and the three osmotic regulation substances show different variation trends at different stages. Appropriate con- centration of NshSiO3 can increase the contents of free proline, soluble sugar and soluble protein to varying degrees, reduce MDA content and further improve the cold resistance of Dendrobium moniliforme plantlets. The order of the effects of Na2SiO~ on various physiological indicators is ： free proline 〉 MDA 〉 soluble sugar （or soluble protein）. According to the correlation analysis among various physiological indicators, free proline, soluble sugar, soluble protein and MDA contents can all be used as reference indicators to identify the cold resistance of Dendrobium moniliforme. [ Conclusion] The addition of Na2SiO3 （0.4 retool/L） can moder- ately decrease the thermal energy for normal growth of Dendrobium moniliforme, which is conducive to reducing the cost of cultivation. Key words Na2SiO3 ;Dendrobium monlifforme;Low temperature stress;Physiological and biochemical characteristics展开更多
探讨采用高内涵筛选技术检测巨噬细胞(RAW264.7)吞噬荧光标记大肠杆菌(GFP-Escherichia coli)方法的灵敏性、稳定性及可靠性,建立中药促进巨噬细胞吞噬活性的高内涵评价方法。对比考察不同的感染复数(multiplicity of infection,MOI)即...探讨采用高内涵筛选技术检测巨噬细胞(RAW264.7)吞噬荧光标记大肠杆菌(GFP-Escherichia coli)方法的灵敏性、稳定性及可靠性,建立中药促进巨噬细胞吞噬活性的高内涵评价方法。对比考察不同的感染复数(multiplicity of infection,MOI)即细菌和细胞比值,以及不同孵育时间对巨噬细胞吞噬细菌能力的影响(吞噬指数=被吞噬的细菌荧光强度/巨噬细胞数),优化高内涵筛选方法和实验参数,并用于测定铜皮石斛对巨噬细胞吞噬作用的影响。结果表明,吞噬指数与MOI值呈正相关,孵育时间在1.5 h吞噬指数最高,优化后的条件:细胞数1×10~4个/孔,孵育时间1.5 h,MOI值为50(即细菌数为5×10~5个/孔),该条件下检测结果的RSD<10%。采用所建立的方法检测铜皮石斛调节巨噬细胞吞噬作用实验的结果显示,在0.31~2.50 g·L^(-1)内铜皮石斛水提液对巨噬细胞吞噬具有剂量依赖的促进作用。本文建立了巨噬细胞吞噬作用评价的高内涵筛选方法,并进一步证明了铜皮石斛水提物促进巨噬细胞的吞噬作用。展开更多
文摘A new bibenzyl derivative, 3,4-dihydroxy-4,5-dimethoxy bibenzyl, was isolated from a orchid Dendrobium moniliforme. The structure elucidation and 1H,13C NMR assignments were achieved by spectroscopic method.
文摘A new bibenzyl glycoside has been isolated from the stems of Dendrobium moniliforme (L.) Sw. (Orchidaceae). Its structure has been identified on the basis of spectroscopic and chemical methods.
基金Supported by College-level Fund of Sichuan Agricultural University(64070113)
文摘[ Objective] The study aimed to investigate the effects of silicon on physiology and biochemistry of Dendrobium moniliforme plantlets under low tempera- ture stress. [ Method ] By using Dendrobium monilforme as the experimental material, different concentrations of Na2SiO3 （0, O. 2, 0.4, 0.6, 0.8, 1.0, 5.0 and 10.0 mmol/L） was added to the basic medium[ 1/2MS ＋ 6-BA （0.1mg/L） ＋ NAA （ 1 mg/L） ＋ agar （7.2 g/L） ＋ sucrose （30 g/L） ] for tissue culture; af- ter hardening and transplanting, Dendrobium moniliforme plantlets were treated under low temperature stress at 4 ~C for 0, 24 and 48 h, in order to investigate the physiological response of Dendrobium ranniliforme leaves to different concentrations of Na2SiO3. [ Result] Under low temperature stress at 4℃, Dendrob/um mon//i- fortns leaves have certain osmotic regulation ability, and the three osmotic regulation substances show different variation trends at different stages. Appropriate con- centration of NshSiO3 can increase the contents of free proline, soluble sugar and soluble protein to varying degrees, reduce MDA content and further improve the cold resistance of Dendrobium moniliforme plantlets. The order of the effects of Na2SiO~ on various physiological indicators is ： free proline 〉 MDA 〉 soluble sugar （or soluble protein）. According to the correlation analysis among various physiological indicators, free proline, soluble sugar, soluble protein and MDA contents can all be used as reference indicators to identify the cold resistance of Dendrobium moniliforme. [ Conclusion] The addition of Na2SiO3 （0.4 retool/L） can moder- ately decrease the thermal energy for normal growth of Dendrobium moniliforme, which is conducive to reducing the cost of cultivation. Key words Na2SiO3 ;Dendrobium monlifforme;Low temperature stress;Physiological and biochemical characteristics
