A multipurpose clone plant species, Hippophae rhamniodes has the capacity for indefinite longevity, although under successive drought stress it may often decline or die across large areas. Field trials were con- ducte...A multipurpose clone plant species, Hippophae rhamniodes has the capacity for indefinite longevity, although under successive drought stress it may often decline or die across large areas. Field trials were con- ducted over 2 years to examine the effects of varied irrigation intensities on modular growth and clonal propagation in a semi-arid area. Irrigation levels included a control, as well as two, four and six times the volume of water that was typically received via local annual average precipitation. Irrigation intensity significantly influenced clonal propagation capacity （number of daughter ramets）, aboveground modular growth （height, base diameter, and crown width）, belowground modular growth included root nodule dry weight, stretching capacity of lateral roots （length of the longest lateral roots, and diameter of first-grade lateral roots）, and branching intensity of lateral roots （number of lateral roots bifurcation grade, number of first- grade lateral roots）. The modular growth and the density of daughter ramets were small under non-irrigation or low irrigation, and became larger with increased irrigation intensity. Beyond a certain threshold, however, further increases in irrigation intensity resulted in a reversion to the development. The optimal irrigation intensities for growth and propagation were 3.48-5.29 times the volume of nominal local annual average precipitation. There were effects of irrigation intensities on the positive significant correlations between aboveground and belowground modular growths, and on clonal propagation capacities. Under various water treatments, H. rhamnoides may adapt to the environment through the regulation of growth and propagation. We concluded that water shortages act to weaken the growth and propagation of H. rhamniodes plantations.展开更多
以建水无籽蜜桔(Citrus reticulate)开花后120天的幼胚为材料。在含BA0.5mg/l(单位以下同)或IBA0.5或BAO.2+2,4—D1.5的MT(Murashige and Tueker)培养基中,可使幼胚发育成丛芽和不同类型的胚状体。IBAO.5—1.0有益于球形胚、子叶胚的增...以建水无籽蜜桔(Citrus reticulate)开花后120天的幼胚为材料。在含BA0.5mg/l(单位以下同)或IBA0.5或BAO.2+2,4—D1.5的MT(Murashige and Tueker)培养基中,可使幼胚发育成丛芽和不同类型的胚状体。IBAO.5—1.0有益于球形胚、子叶胚的增殖,BA0.5有益于子叶胚形成丛芽。小芽段在附加BAO.5的MT培养基中增殖率相对较高,其速度65天为3.2倍,112天为4.2倍,142天为5.2倍。芽苗在1/2MT+1BA(或NAA)0.5—1.0中的平均生根率为90％,接种后置于光下培养比先暗后光处理的生根率要高37.8％;嫩芽苗比老芽苗的生根率高15.4％,且出根早、根数多。移苗的平均成活率为92.2％;移后8个月的苗高达40cm以上。展开更多
基金supported by the National Science Foundation of China(31070551/31570609)
文摘A multipurpose clone plant species, Hippophae rhamniodes has the capacity for indefinite longevity, although under successive drought stress it may often decline or die across large areas. Field trials were con- ducted over 2 years to examine the effects of varied irrigation intensities on modular growth and clonal propagation in a semi-arid area. Irrigation levels included a control, as well as two, four and six times the volume of water that was typically received via local annual average precipitation. Irrigation intensity significantly influenced clonal propagation capacity （number of daughter ramets）, aboveground modular growth （height, base diameter, and crown width）, belowground modular growth included root nodule dry weight, stretching capacity of lateral roots （length of the longest lateral roots, and diameter of first-grade lateral roots）, and branching intensity of lateral roots （number of lateral roots bifurcation grade, number of first- grade lateral roots）. The modular growth and the density of daughter ramets were small under non-irrigation or low irrigation, and became larger with increased irrigation intensity. Beyond a certain threshold, however, further increases in irrigation intensity resulted in a reversion to the development. The optimal irrigation intensities for growth and propagation were 3.48-5.29 times the volume of nominal local annual average precipitation. There were effects of irrigation intensities on the positive significant correlations between aboveground and belowground modular growths, and on clonal propagation capacities. Under various water treatments, H. rhamnoides may adapt to the environment through the regulation of growth and propagation. We concluded that water shortages act to weaken the growth and propagation of H. rhamniodes plantations.
文摘以建水无籽蜜桔(Citrus reticulate)开花后120天的幼胚为材料。在含BA0.5mg/l(单位以下同)或IBA0.5或BAO.2+2,4—D1.5的MT(Murashige and Tueker)培养基中,可使幼胚发育成丛芽和不同类型的胚状体。IBAO.5—1.0有益于球形胚、子叶胚的增殖,BA0.5有益于子叶胚形成丛芽。小芽段在附加BAO.5的MT培养基中增殖率相对较高,其速度65天为3.2倍,112天为4.2倍,142天为5.2倍。芽苗在1/2MT+1BA(或NAA)0.5—1.0中的平均生根率为90％,接种后置于光下培养比先暗后光处理的生根率要高37.8％;嫩芽苗比老芽苗的生根率高15.4％,且出根早、根数多。移苗的平均成活率为92.2％;移后8个月的苗高达40cm以上。
