目的:观察枳实对功能性消化不良(FD)大鼠胃Cajal间质细胞(ICCs)及其SCF/c-Kit信号通路的影响。方法:48只健康SD大鼠随机分为正常组,模型组,枳实低、中、高剂量组,多潘立酮组,每组8只。除正常组外,其余5组均采用改良夹尾刺激法制造FD大...目的:观察枳实对功能性消化不良(FD)大鼠胃Cajal间质细胞(ICCs)及其SCF/c-Kit信号通路的影响。方法:48只健康SD大鼠随机分为正常组,模型组,枳实低、中、高剂量组,多潘立酮组,每组8只。除正常组外,其余5组均采用改良夹尾刺激法制造FD大鼠模型,于造模同一天分别使用枳实低、中、高剂量水煎剂(0.5、1.0、2.0g/m L),多潘立酮(0.30mg/m L)进行灌胃,4周后测定大鼠胃排空率,ELISA法检测血清SCF含量,RT-PCR法检测胃窦组织c-Kit m RNA、SCF m RNA的表达,Western Blot法检测胃窦组织c-Kit蛋白、SCF蛋白的表达水平。结果:与正常组比较,模型组大鼠胃排空率、血清SCF含量、胃窦组织c-Kit m RNA、SCF m RNA表达及胃窦组织c-Kit蛋白、SCF蛋白表达均显著下降(P<0.05);与模型组比较,枳实低、中、高剂量组及多潘立酮组胃排空率、血清SCF含量、胃窦组织c-Kit m RNA、SCF m RNA表达及胃窦组织c-Kit蛋白、SCF蛋白的表达均显著升高(P<0.05);与多潘立酮组比较,枳实低剂量组胃排空率、血清SCF含量、胃窦组织c-Kit m RNA表达、c-Kit蛋白、SCF蛋白的表达均显著降低(P<0.05),枳实中剂量组胃排空率显著降低(P<0.05),胃窦组织SCF m RNA表达及胃窦组织c-Kit蛋白表达均显著升高(P<0.05),枳实高剂量组血清SCF含量、胃窦组织c-Kit m RNA、SCF m RNA表达及胃窦组织c-Kit蛋白、SCF蛋白均显著升高(P<0.05);与枳实低剂量组比较,枳实中、高剂量组胃排空率、血清SCF含量、胃窦组织c-Kit m RNA表达及胃窦组织c-Kit蛋白、SCF蛋白表达均显著升高(P<0.05),枳实中、高剂量组SCF m RNA表达均显著升高(P<0.05);与枳实中剂量组比较,枳实高剂量组胃排空率、血清SCF含量及胃窦组织c-Kit m RNA、SCF m RNA及SCF蛋白表达均显著升高(P<0.05)。结论:枳实可通过上调c-Kit和SCF的表达,启动SCF/c-Kit信号通路,促进ICCs的增殖,从而促胃动力。展开更多
Anxiety is a very common mental disorder among neurological diseases. Some herbs have soothing effects and play an important role in reducing anxiety. The purpose of this study is to investigate the effect of Citrus a...Anxiety is a very common mental disorder among neurological diseases. Some herbs have soothing effects and play an important role in reducing anxiety. The purpose of this study is to investigate the effect of Citrus aurantium L. essential oil on anxiety and its interference with serotonergic pathway. Sixty male mice were assigned into control, sham (saline and olive oil), and experimental groups. Intraperitoneal injection of Citrus aurantium L. essential oil was applied at doses of 0.5, 2.5, and 5 percent for 5 days. In another set of experiments, after intraperitoneal injection of Citrus aurantium L. essential oil at doses of 0.5, 2.5, and 5 percent for 5 days, on the 5th day, 30 minutes before applying essential oil, fluoxetine (2 mg/kg) was injected. Then, the anxiety-related behavior was assessed using elevated plus maze test. The results revealed that injection of essential oil of Citrus aurantium L. alone or along with fluoxetine led to increasing the number of entries into the open arms and the time spent in open arms that was significantly different compared with control and sham groups (P?< 0.001). Besides, further effects revealed when fluoxetine added to essential oils, however no more effects obtained when compared to fluoxetine alone. It is concluded that Citrus aurantium L. essential oil can reduce the anxiety in male mice and due to fluoxetin potentiation and maximum response observed, the herb may express its anxiolytic effects in part, via serotonergic system.展开更多
文摘目的:观察枳实对功能性消化不良(FD)大鼠胃Cajal间质细胞(ICCs)及其SCF/c-Kit信号通路的影响。方法:48只健康SD大鼠随机分为正常组,模型组,枳实低、中、高剂量组,多潘立酮组,每组8只。除正常组外,其余5组均采用改良夹尾刺激法制造FD大鼠模型,于造模同一天分别使用枳实低、中、高剂量水煎剂(0.5、1.0、2.0g/m L),多潘立酮(0.30mg/m L)进行灌胃,4周后测定大鼠胃排空率,ELISA法检测血清SCF含量,RT-PCR法检测胃窦组织c-Kit m RNA、SCF m RNA的表达,Western Blot法检测胃窦组织c-Kit蛋白、SCF蛋白的表达水平。结果:与正常组比较,模型组大鼠胃排空率、血清SCF含量、胃窦组织c-Kit m RNA、SCF m RNA表达及胃窦组织c-Kit蛋白、SCF蛋白表达均显著下降(P<0.05);与模型组比较,枳实低、中、高剂量组及多潘立酮组胃排空率、血清SCF含量、胃窦组织c-Kit m RNA、SCF m RNA表达及胃窦组织c-Kit蛋白、SCF蛋白的表达均显著升高(P<0.05);与多潘立酮组比较,枳实低剂量组胃排空率、血清SCF含量、胃窦组织c-Kit m RNA表达、c-Kit蛋白、SCF蛋白的表达均显著降低(P<0.05),枳实中剂量组胃排空率显著降低(P<0.05),胃窦组织SCF m RNA表达及胃窦组织c-Kit蛋白表达均显著升高(P<0.05),枳实高剂量组血清SCF含量、胃窦组织c-Kit m RNA、SCF m RNA表达及胃窦组织c-Kit蛋白、SCF蛋白均显著升高(P<0.05);与枳实低剂量组比较,枳实中、高剂量组胃排空率、血清SCF含量、胃窦组织c-Kit m RNA表达及胃窦组织c-Kit蛋白、SCF蛋白表达均显著升高(P<0.05),枳实中、高剂量组SCF m RNA表达均显著升高(P<0.05);与枳实中剂量组比较,枳实高剂量组胃排空率、血清SCF含量及胃窦组织c-Kit m RNA、SCF m RNA及SCF蛋白表达均显著升高(P<0.05)。结论:枳实可通过上调c-Kit和SCF的表达,启动SCF/c-Kit信号通路,促进ICCs的增殖,从而促胃动力。
文摘Anxiety is a very common mental disorder among neurological diseases. Some herbs have soothing effects and play an important role in reducing anxiety. The purpose of this study is to investigate the effect of Citrus aurantium L. essential oil on anxiety and its interference with serotonergic pathway. Sixty male mice were assigned into control, sham (saline and olive oil), and experimental groups. Intraperitoneal injection of Citrus aurantium L. essential oil was applied at doses of 0.5, 2.5, and 5 percent for 5 days. In another set of experiments, after intraperitoneal injection of Citrus aurantium L. essential oil at doses of 0.5, 2.5, and 5 percent for 5 days, on the 5th day, 30 minutes before applying essential oil, fluoxetine (2 mg/kg) was injected. Then, the anxiety-related behavior was assessed using elevated plus maze test. The results revealed that injection of essential oil of Citrus aurantium L. alone or along with fluoxetine led to increasing the number of entries into the open arms and the time spent in open arms that was significantly different compared with control and sham groups (P?< 0.001). Besides, further effects revealed when fluoxetine added to essential oils, however no more effects obtained when compared to fluoxetine alone. It is concluded that Citrus aurantium L. essential oil can reduce the anxiety in male mice and due to fluoxetin potentiation and maximum response observed, the herb may express its anxiolytic effects in part, via serotonergic system.
