摘要
为探究和比较分心木、赶黄草、青蒿和代代花4种植物的抗氧化活性,以4种植物的提取物为试验材料,采用福林酚法和三氯化铝比色法测定总黄酮和总酚含量。以DPPH自由基清除率、ABTS+自由基清除率和总抗氧化能力为指标,进行抗氧化活性评价。研究结果表明,4种植物提取物的总黄酮和总酚的含量、抗氧化活性差异显著。其中,分心木提取物的总黄酮含量最高,可达(21.87±0.55)mg RT/g,且DPPH自由基清除能力、总抗氧化能力最强;赶黄草提取物的总酚含量最高,可达(14.78±0.95)mg GAE/g,且ABTS+自由基清除能力最强。4种植物提取物均具有一定程度的抗氧化能力,赶黄草和分心木提取物的抗氧化活性明显高于青蒿和代代花提取物,抗氧化能力与其总黄酮含量呈正相关。
The aim of this study was to explore and compare the antioxidative activities of four plants,namely Penthorum chinense Pursh,Diaphragma juglandis Fructus,Artemisia annua L.,and Citrus aurantium L.,the extracts of which were used as test materials.The total polyphenol and flavonoid contents were determined using the Folin-Ciocaileu reagent method and aluminum trichloride colorimetry,respectively.The antioxidant activity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl(DPPH)free radical scavenging rate,2,2’-azinobis-(3-ethylbenzothiazoline-6-sulphonate)(ABTS+)free radical scavenging rate,and total antioxidant capacity.The results showed that there were significant differences in the total flavonoid and phenol contents and antioxidant activities of the four plant species.The total flavonoid content of D.juglandis Fructus extract was the highest(21.87±0.55)mg RT/g and the DPPH free radical rate and total antioxidation were the strongest,whereas the total phenol content of P.chinense Pursh extract was the highest(14.78±0.95)mg GAE/g and the ABTS+free radical scavenging rate was the strongest.The extracts of each of the four plant species exhibited a specific antioxidative capacities,and those of P.chinense Pursh and D.juglandis Fructus were significantly higher than those of A.annua L.and C.aurantium L.The antioxidant capacity was also positively correlated with the total flavonoid content.
作者
徐韶棠
杨轶浠
王艺瑾
郭育涛
刘雅宁
张佳敏
覃小颖
张珏
XU Shao-tang;YANG Yi-xi;WANG Yi-jin;GUO Yu-tao;LIU Ya-ning;ZHANG Jia-min;QIN Xiao-ying;ZHANG Jue(College of Food and Biological Engineering,Chengdu University,Chengdu 610106,Sichuan,China;Key Laboratory of Meat Processing in Sichuan Province,Chengdu University,Chengdu 610106,Sichuan,China;Sichuan Liangyuan Food Limited Company,Bazhong 636000,Sichuan,China)
出处
《食品研究与开发》
CAS
北大核心
2022年第24期66-72,共7页
Food Research and Development
基金
西藏自治区科技计划项目重点研发计划(XZ202201ZY0031G)
四川省科技厅“三区”人才项目
成都市科技局技术创新研发项目(2021-YF05-00358-SN)
川菜发展研究中心规划项目(CC21Z03)
四川省肉类加工重点实验室开放基金项目(16R-10)。
关键词
抗氧化活性
赶黄草
分心木
青蒿
代代花
总黄酮
总酚
antioxidative activity
Penthorum chinense Pursh
Diaphragma juglandis Fructus
Artemisia annua L.
Citrus aurantium L.
total flavonoids
total phenol
