This research reports on an efficient shoot proliferation and callus regeneration system for bamboo.Young, semi-lignified branches with one lateral bud from Drepanostachyum luodianense(Yi et R. S. Wang) Keng f.were us...This research reports on an efficient shoot proliferation and callus regeneration system for bamboo.Young, semi-lignified branches with one lateral bud from Drepanostachyum luodianense(Yi et R. S. Wang) Keng f.were used as explants. Disinfection with 0.1% HgCl2 for 8 min was the optimum treatment and the best medium for bud initiation was Murashige and Skoog(MS) medium containing 3.0 mg L-16-benzyladenine(BA). Multiple shoots were induced from nodal shoot segments on MS medium containing 5.0 mg L-1 BA, 0.5 mg L-1 kinetin(Kin), and 1.0 mg L-1 naphthaleneacetic acid(NAA). The highest frequency of callus formation(65.6%) was on MS medium containing 4.0 mg L-12,4-dichlorophenoxyacetic acid(2, 4-D), 0.5 mg L-1 NAA, and 0.1 mg L-1 thidiazuron(TDZ). The optimum medium for callus proliferation was MS medium with 4 mg L-12,4-D, 0.5 mg L-1 TDZ and 0.5 mg L-1 NAA, and the optimum hormone combination was 4 mg L-1 BA ? 0.5 mg L-1 NAA for callus redifferentiation(up to 85.6%). A 100% rooting was achieved on MS medium supplemented with 2.0 mg L-1 NAA and 0.5 mg L-13-indole butyric acid(IBA). Rooted plantlets were acclimatized in a greenhouse in humus soil ? perlite(1:1) substrate. These micropropagated callus induction and regeneration systems for bamboo will be useful for genetic engineering and multiplication.展开更多
基金supported by the National Key Research and Development Program of China under Grant2016YFD0600901Jiangsu Province ‘‘SANXIN’’ Support Project under Grant LYSX [2016]04+1 种基金National Natural Science Foundation for Young Scholars of China under Grant 31000294Priority Academic Program Development of Jiangsu Higher Education Institutions
文摘This research reports on an efficient shoot proliferation and callus regeneration system for bamboo.Young, semi-lignified branches with one lateral bud from Drepanostachyum luodianense(Yi et R. S. Wang) Keng f.were used as explants. Disinfection with 0.1% HgCl2 for 8 min was the optimum treatment and the best medium for bud initiation was Murashige and Skoog(MS) medium containing 3.0 mg L-16-benzyladenine(BA). Multiple shoots were induced from nodal shoot segments on MS medium containing 5.0 mg L-1 BA, 0.5 mg L-1 kinetin(Kin), and 1.0 mg L-1 naphthaleneacetic acid(NAA). The highest frequency of callus formation(65.6%) was on MS medium containing 4.0 mg L-12,4-dichlorophenoxyacetic acid(2, 4-D), 0.5 mg L-1 NAA, and 0.1 mg L-1 thidiazuron(TDZ). The optimum medium for callus proliferation was MS medium with 4 mg L-12,4-D, 0.5 mg L-1 TDZ and 0.5 mg L-1 NAA, and the optimum hormone combination was 4 mg L-1 BA ? 0.5 mg L-1 NAA for callus redifferentiation(up to 85.6%). A 100% rooting was achieved on MS medium supplemented with 2.0 mg L-1 NAA and 0.5 mg L-13-indole butyric acid(IBA). Rooted plantlets were acclimatized in a greenhouse in humus soil ? perlite(1:1) substrate. These micropropagated callus induction and regeneration systems for bamboo will be useful for genetic engineering and multiplication.
