摘要
本试验以曼陀罗茎段为外植体,在附加不同植物激素组合的培养基中对愈伤组织的诱导和植株再生进行研究。结果表明:采用修改的MS培养基(除去甘氨酸,维生素B1含量增加至0.5mg/L,pH5.5)附加2mg/L2,4-D可由曼陀罗茎段诱导大量胚性愈伤组织;愈伤组织继代选用0.5mg/L2,4-D为宜;不定芽的诱导采用MS培养基(20g蔗糖,8g琼脂,0.1g水解干酪素)+6-BA(0.5mg/L);幼苗进一步转接至1/2MS+IBA(0.2mg/L)生根培养基中,可完成曼陀罗茎段愈伤组织诱导和再生植株的组织培养过程。
The callus induction and plantlet regeneration were researched on the different plant hormone combination media with Datura metel L.stem as explants.The results showed as below：Datura metel L.callus were induced on the modified MS containing vitamin B1 0.5mg/L and 2,4-D 2mg/L without glycin,pH5.5.The Datura metel L.callus were subcultured on the modified MS containing 2,4-D 0.5mg/L.The plantlet regeneration were performed on the MS medium containing sucrose 20g,hydrolyze casein 0.1g and 0.5mg/L 6-BA.1/2MS medium containing 0.2mg/L IBA was suitable for root growth.
出处
《生物技术通报》
CAS
CSCD
2007年第5期179-183,共5页
Biotechnology Bulletin
关键词
曼陀罗
愈伤组织
诱导
组织培养
Datura metel L. Callus induction Plantlet Regeneration
